Difference between revisions of "Team:ETH Zurich/Composite Part"

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<h2>Composite Parts</h2></div>
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<div>
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<h1>COMPOSITE PARTS</h1>
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</div>
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<div class="sec white">
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<div>
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<table>
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<th>Part Name</th>
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<th>Biobrick</th>
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<th>Comments</th>
  
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<p>
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</tr>
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List of composite parts:
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<tr>
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<ul>
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<td>AHL regulated sfgfp expression</td>
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116024">Dual fluorescence reporter for bxb1 integrase</a></li>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116070">BBa_K2116070</a></td>
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116028">bxb1 integrate under the control of nitric oxide and AHL responsive AND gate</a></li>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_J23118">BBa_J23118</a> promoter with a downstream <b>esabox</b>,
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116031">PnorV_gfp (p6)</a></li>
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in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>. Expression induced in
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116032">j23118_esar (p9)</a></li>
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presence of the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116055">CAT-UPRT Fusion with sfGFP</a></li>
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</tr>
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116056">J23102_esaR</a></li>
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            <tr>
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116057">bxb1 pTet</a></li>
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<td>AHLregulated sfgfp expression</td>
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116058">tp901 pTet</a></li>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116052">BBa_K2116052</a></td>
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116059">and gate_bxb1(medium tag)</a></li>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_J23118">BBa_J23118</a> promoter with <b>two downstream esaboxes 8bp apart</b>,
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116060">AND gate bxb1 (fast tag)</a></li>
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in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>. Expression induced in
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116061">Ptet bxb1 (med tag)</a></li>
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presence of the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116062">Ptet tp901(med tag)</a></li>
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</tr>
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116063">Ptet bxb1 (no tag)</a></li>
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            <tr>
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116064">Ptet tp901(no tag)</a></li>
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<td>AHL regulated sfgfp expression</td>
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<li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116065">phiC31 reporter p114</a></li>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116071">BBa_K2116071</a></td>
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</ul>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_J23118">BBa_J23118</a> promoter with <b>three downstream esaboxes 8bp apart</b>,
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</p>
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in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>. Expression induced in
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presence of the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
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</tr>
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            <tr>
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<td>AHL regulated sfgfp expression</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116072">BBa_K2116072</a></td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_J23118">BBa_J23118</a> promoter with <b>two downstream esaboxes 15bp apart</b>,
 +
in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>. Expression induced in
 +
presence of the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
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</tr>
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<tr>
 +
<td>AHL regulated sfgfp expression</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116073">BBa_K2116073</a></td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_J23118">BBa_J23118</a> promoter with <b>three downstream esaboxes 15bp apart</b>,
 +
in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>. Expression induced in
 +
presence of the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
 +
</tr>
 +
            <tr>
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<td>Nitric Oxide <b>and</b> AHL regulated sfgfp expression</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116074">BBa_K2116074</a></td>
 +
<td>Nitric oxide responsive <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116002">PnorV</a>  promoter <b>with</b> its native spacer and <b>one downstream esabox</b>, in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>.
 +
Expression induced in presence of both nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
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</tr>
 +
            <tr>
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<td>Nitric Oxide <b>and</b> AHL regulated sfgfp expression</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116075">BBa_K2116075</a></td>
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<td>Nitric oxide responsive <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116002">PnorV</a> promoter <b>with</b> its native spacer and <b>two downstream esaboxes 8bp apart</b>, in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>.
 +
Expression induced in presence of both nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
 +
</tr>
 +
                <tr>
 +
<td>Nitric Oxide <b>and</b> AHL regulated sfgfp expression</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116076">BBa_K2116076</a></td>
 +
<td>Nitric oxide responsive <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116002">PnorV</a>  promoter <b>with</b> its native spacer and <b>three downstream esaboxes 8bp apart</b>, in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>.
 +
Expression induced in presence of both nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
 +
</tr>
 +
            <tr>
 +
<td>Nitric Oxide <b>and</b> AHL regulated sfgfp expression</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116077">BBa_K2116077</a></td>
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<td>Nitric oxide responsive <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116002">PnorV</a> promoter <b>without</b> its native spacer and <b>one downstream esabox</b>, in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>.
 +
Expression induced in presence of both nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
 +
</tr>
 +
              <tr>
 +
<td>Nitric Oxide <b>and</b> AHL regulated sfgfp expression</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116078">BBa_K2116078</a></td>
 +
<td>Nitric oxide responsive <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116002">PnorV</a>  promoter <b>without</b> its native spacer and <b>two downstream esaboxes 8bp apart</b>, in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>.
 +
Expression induced in presence of both nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
 +
</tr>
 +
              <tr>
 +
<td>Nitric Oxide <b>and</b> AHL regulated sfgfp expression</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116079">BBa_K2116079</a></td>
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<td>Nitric oxide responsive <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116002">PnorV</a> promoter <b>without</b> its native spacer and <b>three downstream esaboxes 8bp apart</b>, in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>.
 +
Expression induced in presence of both nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
 +
</tr>
 +
            <tr>
 +
<td>Nitric Oxide <b>and</b> AHL regulated sfgfp expression</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116080">BBa_K2116080</a></td>
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<td>Nitric oxide responsive <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116002">PnorV</a> promoter <b>with</b> its native spacer and <b>two downstream esaboxes 15bp apart</b>, in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>.
 +
Expression induced in presence of both nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
 +
</tr>
 +
            <tr>
 +
<td>Nitric Oxide <b>and</b> AHL regulated sfgfp expression</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116081">BBa_K2116081</a></td>
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<td>Nitric oxide responsive <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116002">PnorV</a> promoter <b>with</b> its native spacer and <b>three downstream esaboxes 15bp apart</b>, in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>.
 +
Expression induced in presence of both nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
 +
</tr>
 +
            <tr>
 +
<td>Nitric Oxide <b>and</b> AHL regulated sfgfp expression</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116082">BBa_K2116082</a></td>
 +
<td>Nitric oxide responsive <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116002">PnorV</a>  promoter <b>without</b> its native spacer and <b>two downstream esaboxes 15bp apart</b>, in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>.
 +
Expression induced in presence of both nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
 +
</tr>
 +
            <tr>
 +
<td>Nitric Oxide <b>and</b> AHL regulated sfgfp expression</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116083">BBa_K2116083</a></td>
 +
<td>Nitric oxide responsive <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116002">PnorV</a> promoter <b>without</b> its native spacer and <b>three downstream esaboxes 15bp apart</b>, in front of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">sfgfp</a>.
 +
Expression induced in presence of both nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL. </td>
 +
</tr>
 +
              <tr>
 +
<td>bxb1 integrase under Ptet promoter</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116056">BBa_K2116056</a></td>
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<td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K21160009">bxb1 integrase</a> with a fast ssrA degradation tag, under the control of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_R0040">Ptet</a>.
 +
</tr>
 +
</table>
 +
</div>
 
</div>
 
</div>
  

Revision as of 09:40, 1 December 2016

PAVLOV'S COLI

COMPOSITE PARTS

Part Name Biobrick Comments
AHL regulated sfgfp expression BBa_K2116070 BBa_J23118 promoter with a downstream esabox, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
AHLregulated sfgfp expression BBa_K2116052 BBa_J23118 promoter with two downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
AHL regulated sfgfp expression BBa_K2116071 BBa_J23118 promoter with three downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
AHL regulated sfgfp expression BBa_K2116072 BBa_J23118 promoter with two downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
AHL regulated sfgfp expression BBa_K2116073 BBa_J23118 promoter with three downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116074 Nitric oxide responsive PnorV promoter with its native spacer and one downstream esabox, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116075 Nitric oxide responsive PnorV promoter with its native spacer and two downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116076 Nitric oxide responsive PnorV promoter with its native spacer and three downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116077 Nitric oxide responsive PnorV promoter without its native spacer and one downstream esabox, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116078 Nitric oxide responsive PnorV promoter without its native spacer and two downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116079 Nitric oxide responsive PnorV promoter without its native spacer and three downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116080 Nitric oxide responsive PnorV promoter with its native spacer and two downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116081 Nitric oxide responsive PnorV promoter with its native spacer and three downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116082 Nitric oxide responsive PnorV promoter without its native spacer and two downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression BBa_K2116083 Nitric oxide responsive PnorV promoter without its native spacer and three downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
bxb1 integrase under Ptet promoter BBa_K2116056 bxb1 integrase with a fast ssrA degradation tag, under the control of Ptet.

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