Difference between revisions of "Team:SCSU-New Haven/Experiments"

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<h1>Experiments</h1>
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You have the parts. You do the tests to get the numbers. Y
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<h2>Detector Assay</h2>
  
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<p>Describe the experiments, research and protocols you used in your iGEM project.</p>
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<p class="header_intro"> The Detector assay will be done by placing GFP downstream of Psal and exposing the cells to 3-Methylsalicylate. The luminescence response will be measured. We would like to see a response from 0.1uM and less of 3-Methylsalicylate as this was the amount required to produce a response in the paper we obtained the sequence from.
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<img src="https://static.igem.org/mediawiki/2016/9/9b/SCSU-New_HavenDetectorAssay.jpeg">  
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<h5>What should this page contain?</h5>
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</div><h2>Feedback Assay</h2>
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<li> Protocols </li>
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<h5>Inspiration</h5>
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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<p class="header_intro"> The Assay for the Feedback part will be similar to the Detector Assay. The Feedback part will be place downstream of Psal and upstream of GFP and luminescence will be measured. If the part is working as intended, we would see a rise in luminescence to a steady state. This means the feedback loop is turning the Psal promoter on and keeping it on as intended.
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                                  </div><h2>Color Reporter Assays</h2>
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<p class="header_intro"> The Assay for color reporter will be as the above assays substituting the GFP for the Color Reporter part. The production of Indole will be measured using a spectrophotometer. We should expect to see production of Indole comparable to luminescence.  The Degrader would then be added and measurements of Indigo Dye would be taken. Comparing these results to the production of indole would tell us how well the Degrader enzymes convert indole to indigo dye in the system.
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Revision as of 02:17, 20 October 2016

SCSU IGEM SCSU iGEM

Experiments

You have the parts. You do the tests to get the numbers. Y

Detector Assay

The Detector assay will be done by placing GFP downstream of Psal and exposing the cells to 3-Methylsalicylate. The luminescence response will be measured. We would like to see a response from 0.1uM and less of 3-Methylsalicylate as this was the amount required to produce a response in the paper we obtained the sequence from.

Feedback Assay

The Assay for the Feedback part will be similar to the Detector Assay. The Feedback part will be place downstream of Psal and upstream of GFP and luminescence will be measured. If the part is working as intended, we would see a rise in luminescence to a steady state. This means the feedback loop is turning the Psal promoter on and keeping it on as intended.

Color Reporter Assays

The Assay for color reporter will be as the above assays substituting the GFP for the Color Reporter part. The production of Indole will be measured using a spectrophotometer. We should expect to see production of Indole comparable to luminescence. The Degrader would then be added and measurements of Indigo Dye would be taken. Comparing these results to the production of indole would tell us how well the Degrader enzymes convert indole to indigo dye in the system.