Our team divide the project into 4 parts as below.
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Plasmids are losing, and the production efficiency is decreasing. Let’s equip the bacteria with a plasmid-sensing logically adjustable cell killer (P-SLACKiller) to kill slackers with less plasmids among bacteria!
In what situation can the bacteria be judged as slackers? There must have a threshold concerning plasmid numbers. Let’s find it out and adjust it accordingly.
For the slackers, they should be able to turn on the self-killing switch. We tried toxin proteins to produce the lethal effect.
The executor killer should be stably maintained, so we integrated killer device into the genome. Moreover, we mutated promoters to adjust the threshold.