Team:CSU Fort Collins/NoteBook/Sep

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CSU iGEM



September

9/6/16:
  • Extracted Sll0208 and Sll0209 from Synechocystis pcc 6803
  • Ran PCR products from Qblock on 1% gel
9/10/16:
  • PCRed out SLr0168+Luc out of SLr0168+Luc+pSB1C3
  • Phosphorylated Slr0168+Luc
  • Performed ligase cycling on phosphorylated mixture to add T7 promoter and ribosome binding site
  • Transformed ligase cycling mixture
9/14/16:
  • PCRed out SLr0168+Luc out of SLr0168+Luc+pSB1C3
  • Checked PCR products on 1% gel
  • DPN1 digested PCR products
  • Phosphorylated Slr0168+Luc
  • Performed Ligase cycling reaction on phosphorylated mixture to add T7 promoter and rbs
  • Transformed ligase cycling product
9/16/16:
  • Colony PCR on Slr0168+Luc+T7 Promoter+ RBS transformed colonies with Q5 master mix
  • Checked PCR products on 1% gel
9/21/16:
  • DPN1 digested Sll0517 and Sll1633
9/22/16:
  • Phosphorylated parts for two and gates
  • Mix1: Slr0168+Luc+T7 Promoter+ RBS, T7-N ,T7-C,Siga,Lac,Psll0517
  • Mix2: Slr0168+Luc+T7 Promoter+ RBS, T7-N ,T7-C,Siga,Lac,Psll1633
  • Ligase Cycling was performed on the phosphorylated mixtures
  • Transformed ligase cycling mixture

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