Team:CSU Fort Collins/NoteBook/Oct
CSU iGEM
October
10/4/16:
- PCR amplified T7N, T7C, and Slr0168+Luc+T7Promoter+rbs for LCR
- Ran PCR results on a 1% gel
10/5/16:
- PCR amplified Tic 2 op and Siga for LCR
- Ran PCR results on a 1% gel
- PCR amplified Slr0168+Luc+T7Promoter+rbs
- Ran on a 1% gel
10/6/16:
- DPN1 digested T7N,T7C,Slr0168+Luc+T7Promoter+rbs,Tic 2 op,Siga
- Phosphorylated DPN1 digestion products for LCR
- Performed LCR on Phosphorylated mixture
- Transformed LCR products
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