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Team:Stockholm/Protocols

Protocols

Below are the protocols we used in this project.

3A Assembly (Digestion and Ligation)

Biofilm assay

Buffer Preparation, Media, Agar, Agarose, TAE buffer

Collecting BioBrick DNA

Colony Picking

Gel Electrophoresis

Gel Purification

Gibson Assembly

Glycerol Stocks

IMAC Purification

Kirby-Bauer test

PCR Amplification using Phusion Polymerase

PCR Amplification using Taq Polymerase

PCR Amplification using Q5 Polymerase

PCR Purification

Plasmid Extraction

Protein Expression

SDS-PAGE

Sonication

Sortase A Conjugation

Testing Competence of Cells

Thermolysin digestion

Transformation


iGEM Stockholm is sponsored by
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