Team:Macquarie Australia/SDSpage

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SDS-PAGE



  1. Re-suspend pelleted bacterial cells in 200µL of Milli-Q-water
  2. Transfer 50 µL of suspension into new Eppendorf tubes and combine with 50µL of 2xTruSep sample buffer.
  3. Shear the cells using a Hamilton syringe.
  4. Centrifuge the preparation for 3 minutes at 13,000 rpm.
  5. Load 20 µL of the supernatant into gel.
  6. Conduct electrophoresis at a constant voltage (200V) for 1 hour.
  7. Coommassie Stain for ~30 minutes.