Team:Macquarie Australia/ligation

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Ligation



  1. Firstly, 2µl of digested Plasmid Backbone an equimolar amount of digested Part A (< 3µl) and an equimolar amount of digested Part B (< 3µl) were added to 1µl T4 DNA ligase buffer and 0.5µl of T4 DNA ligase.
  2. Water was added to bring the total to 10µl.
  3. Ligation was performed for 30 minutes at 16℃ followed by a further period of 20 minutes at 80℃.