1. PETase is nontoxic
Our project is on the basis of PETase and its respective mutants.PETase is isolated from a novel bacterium,Ideonella sakaiensis 201-F6, that is able to use PET as its major energy and carbon source. Thus, we have good reasons to believe there isn't any safety problem about PETase.
2. Our new applications are more environment-friendly
About our Trainning Protein project, there is no doubt that it is environment-friendly. It not only helps to solve the plastic pollution problem, using the ezymolysis way not just landfill, incineration and chemical decomposition, but also enhances its effect.
In order to avoid danger and train our team member, everyone accepted the biological safety test even some of our members in charge of wiki. Of course, they all qualified. What’s more, when we formally conducted experiments, every member will be supervised by our instructors and each other. If we found who conducted work nonstandardly, we will punish him/her by being on duty at that day. And all our waste are discarded safely, strictly followed stipulations of our school.
We chose BL21 (DE3) and Pichia Pastoris as our expressing strain, and we first constructed expression vectors in E. coli DH5alpha. In the process, use kits which are safe to extract, construct and transform our plasmids. ALL THE ORGANISM we use in our lab are safety level 1 organisms. We conducted our operations on open clean benches and all the waste contains microorganisms are sterilized before further dispose to ensure safety. According to the situation, we will choose to wear protective equipment.
And the following is our regulations of lab:
Be sure that you have worn protective equipment if necessary.
Be sure that you have informed the instructor of the next step of the experiment plan and gotten the permission of him.
Keep the super clean bench clean and operate correctly.
Properly handle the waste generated in the experiment.
Clean the table after the experiment.
Make the experiment record and finish experiment results in time.
To achieve safe shipment, we followed the iGEM official advice for Teams in China to send the samples to GenScript in Nanjin,China which is quick and convenient.