Difference between revisions of "Team:Edinburgh UG/Plate Reader"

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                     <p>The protocol supplied in the iGEM 2015 InterLab Protocol  form was followed with a few minor changes.
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                     <p>text 1
 
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                     The aim of the 2016 iGEM InterLab was to quantify the expression of GFP under different promoters and Ribosomal Binding Regions (RBRs). The use of GFP to indirectly measure the expression of a specific protein in cells is a key approach in biology. It enables us to monitor expression levels without killing the cells. The ability measure expression levels per cell is important to be able to quantify strength of promotors. In order to achieve this, population size is indirectly measured using OD600. Expression per cell is then obtained by Fluorescence/OD600.</p>
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                     Demo 1</p>
  
 
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Revision as of 09:26, 1 September 2016

Team:Edinburgh/InterLab - 2015.igem.org

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Team:Edinburgh/InterLab

InterLab Study

Introduction

The term InterLab stands for Inter-Laboratory studies. The iGEM InterLab studies conducted duringthe last two years have been of huge importance to establish a standard for reproducibility offluorescent measurements. The measurement of fluorescence is an essential tool in synthetic biology.However, the absence of absolute units and standard protocols makes it harder to interpret data and share biological parts between laboratories. The 2016 iGEM InterLab consists of two protocols formeasuring GFP that will lead to the creation of absolute units. This collection of measurements can then be used to establish the accuracy with which GFP is measured around the world and what needs to be improved when following a standard protocol. As a team, we decided that the iGEM InterLab was a great opportunity to improve our laboratory skills and take part into an international study.



More detailed information regarding the cloning process can be found below and on our InterLab lab notebook page.

Plate Reader

All other protocols used in the process of the study can be found on the protocols page.

Introduction

text 1
Demo 1

Demo

This is a demo.
This is a demo.

Demo

Demo.

Demo

Demo

Results

Demo

Demo

Demo

1.) 1278.712
2.) 1216.887
3.) 1110.834

Demo

1.) 3185.188
2.) 3119.913
3.) 2396.115
Mean = 2900.405
Standard Deviation = 437.946

Demo - Something else

Demo

2nd Demo
Demo