Difference between revisions of "Team:Tongji Shanghai/Results"

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<head>
  
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<div class="column full_size" >
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  <!-- Basic -->
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  <title>Margo | Blog</title>
  
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<p>Here you can describe the results of your project and your future plans. </p>
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<h5>What should this page contain?</h5>
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<ul>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project </li>
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<li> Considerations for replicating the experiments </li>
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</ul>
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<div class="column half_size" >
 
  
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  <link rel="stylesheet" href="asset/css/bootstrap.min.css" type="text/css" media="screen">
  
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<h5> Project Achievements </h5>
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  <link rel="stylesheet" href="css/font-awesome.min.css" type="text/css" media="screen">
  
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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  <link rel="stylesheet" type="text/css" href="css/slicknav.css" media="screen">
  
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<ul>
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<li>A list of linked bullet points of the successful results during your project</li>
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  <link rel="stylesheet" type="text/css" href="css/style.css" media="screen">
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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</ul>
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  <!-- Responsive CSS Styles  -->
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  <link rel="stylesheet" type="text/css" href="css/colors/blue.css" title="blue" media="screen" />
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  <link rel="stylesheet" type="text/css" href="css/colors/beige.css" title="beige" media="screen" />
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  <link rel="stylesheet" type="text/css" href="css/colors/cyan.css" title="cyan" media="screen" />
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  <link rel="stylesheet" type="text/css" href="css/colors/green.css" title="green" media="screen" />
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  <link rel="stylesheet" type="text/css" href="css/colors/orange.css" title="orange" media="screen" />
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  <link rel="stylesheet" type="text/css" href="css/colors/peach.css" title="peach" media="screen" />
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  <link rel="stylesheet" type="text/css" href="css/colors/pink.css" title="pink" media="screen" />
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  <link rel="stylesheet" type="text/css" href="css/colors/purple.css" title="purple" media="screen" />
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  <link rel="stylesheet" type="text/css" href="css/colors/sky-blue.css" title="sky-blue" media="screen" />
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  <link rel="stylesheet" type="text/css" href="css/colors/yellow.css" title="yellow" media="screen" />
  
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<div class="column half_size" >
 
  
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<h5>Inspiration</h5>
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<p>See how other teams presented their results.</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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  <script type="text/javascript" src="js/modernizrr.js"></script>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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  <script type="text/javascript" src="asset/js/bootstrap.min.js"></script>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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  <script type="text/javascript" src="js/jquery.fitvids.js"></script>
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</ul>
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            <h2>Achievment</h2>
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            <p>Why we deserve a medal.</p>
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              <li>Achievement</li>
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              <h4>Judging<span class="head-line"></span></h4>
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                  <a href="#">Topic 1</a>
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                </li>
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                <li>
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                  <a href="#">Topic 2</a>
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                  <a href="#">Topic 3</a>
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              <h3 class="classic-title" style="margin-top:0px"><span><a  href="https://2015.igem.org/Team:Peking/Design/PC_Reporter">Paired dCas9 reporter</a></span></h3>
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              <div style="margin-top:0px; margin-bottom:0">
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                <div class="row">
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                    <img style="max-with:400px;" src="https://static.igem.org/mediawiki/2015/4/4e/Peking-Project-Overview1.png">
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                  </div>
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                  <div class="col-md-6">
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                <p> It is well known that CRISPR/dCas9 has a unique ability to be programmed to bind any sequence with the assistance of sgRNA; it was conventionally used for DNA editing or genome study. In our project, however, we integrate split reporters into CRISPR/Cas9 by translationally fusing two fragments of a split reporter to dCas9, respectively, to convert the sequence-specific information of pathogenic bacteria's genome (in our case, <i>M. tuberculosis</i>) into easily readable signal including bioluminescence or pigment. We demonstrated that the PC reporter is highly compatible with NAD-based diagnosis using isolated genomic DNA of MTB.</p>
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                </div>
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              </div>
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            </div>
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              <h3 class="classic-title" style="margin-top:0px"><span><a href="https://2015.igem.org/Team:Peking/Design/PC_Reporter">Paired dCas9 reporter</a></span></h3>
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              <div style="margin-top:0px; margin-bottom:0">
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                <div class="row">
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                  <div class="col-md-6">
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                    <img style="max-with:400px;" src="https://static.igem.org/mediawiki/2015/4/4e/Peking-Project-Overview1.png">
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                  </div>
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                  <div class="col-md-6">
 +
                <p> It is well known that CRISPR/dCas9 has a unique ability to be programmed to bind any sequence with the assistance of sgRNA; it was conventionally used for DNA editing or genome study. In our project, however, we integrate split reporters into CRISPR/Cas9 by translationally fusing two fragments of a split reporter to dCas9, respectively, to convert the sequence-specific information of pathogenic bacteria's genome (in our case, <i>M. tuberculosis</i>) into easily readable signal including bioluminescence or pigment. We demonstrated that the PC reporter is highly compatible with NAD-based diagnosis using isolated genomic DNA of MTB.</p>
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                </div>
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              </div>
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            </div>           
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          </div>
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        </div>
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</div>
 
</div>
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</div>             
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        <a onClick="setActiveStyleSheet('blue'); return false;" title="Blue" class="blue"></a>
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        <a onClick="setActiveStyleSheet('sky-blue'); return false;" title="Sky Blue" class="sky-blue"></a>
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        <a onClick="setActiveStyleSheet('cyan'); return false;" title="Cyan" class="cyan"></a>
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        <a onClick="setActiveStyleSheet('jade'); return false;" title="Jade" class="jade"></a>
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        <a onClick="setActiveStyleSheet('green'); return false;" title="Green" class="green"></a>
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        <a onClick="setActiveStyleSheet('purple'); return false;" title="Purple" class="purple"></a>
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        <a onClick="setActiveStyleSheet('pink'); return false;" title="Pink" class="pink"></a>
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        <a onClick="setActiveStyleSheet('red'); return false;" title="Red" class="red"></a>
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        <a onClick="setActiveStyleSheet('orange'); return false;" title="Orange" class="orange"></a>
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        <a onClick="setActiveStyleSheet('yellow'); return false;" title="Yellow" class="yellow"></a>
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        <a onClick="setActiveStyleSheet('peach'); return false;" title="Peach" class="peach"></a>
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        <a onClick="setActiveStyleSheet('beige'); return false;" title="Biege" class="beige"></a>
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    <span>Top Bar Color</span>
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    <select id="topbar-style" class="topbar-style">
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      <option value="1">Light (Default)</option>
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      <option value="2">Dark</option>
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      <option value="3">Color</option>
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    <span>Layout Style</span>
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    <select id="layout-style" class="layout-style">
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      <option value="1">Wide</option>
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      <option value="2">Boxed</option>
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    <span>Patterns for Boxed Version</span>
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    <ul class="bg-list">
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        <a href="#" class="bg1"></a>
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        <a href="#" class="bg7"></a>
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        <a href="#" class="bg8"></a>
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        <a href="#" class="bg9"></a>
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        <a href="#" class="bg10"></a>
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{{Team:Tongji_Shanghai/footer}}

Revision as of 03:26, 16 October 2016

Tongji_Shanghai-2016.igem.org Tongji Shanghai

Margo | Blog

Achievment

Why we deserve a medal.

Paired dCas9 reporter

It is well known that CRISPR/dCas9 has a unique ability to be programmed to bind any sequence with the assistance of sgRNA; it was conventionally used for DNA editing or genome study. In our project, however, we integrate split reporters into CRISPR/Cas9 by translationally fusing two fragments of a split reporter to dCas9, respectively, to convert the sequence-specific information of pathogenic bacteria's genome (in our case, M. tuberculosis) into easily readable signal including bioluminescence or pigment. We demonstrated that the PC reporter is highly compatible with NAD-based diagnosis using isolated genomic DNA of MTB.

Paired dCas9 reporter

It is well known that CRISPR/dCas9 has a unique ability to be programmed to bind any sequence with the assistance of sgRNA; it was conventionally used for DNA editing or genome study. In our project, however, we integrate split reporters into CRISPR/Cas9 by translationally fusing two fragments of a split reporter to dCas9, respectively, to convert the sequence-specific information of pathogenic bacteria's genome (in our case, M. tuberculosis) into easily readable signal including bioluminescence or pigment. We demonstrated that the PC reporter is highly compatible with NAD-based diagnosis using isolated genomic DNA of MTB.

Style Switcher

12 Predefined Color Skins
Top Bar Color Layout Style Patterns for Boxed Version