Difference between revisions of "Team:RHIT/Description"
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<h3> General Overview </h3> | <h3> General Overview </h3> | ||
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<h3>Results (We Hope)</h3> | <h3>Results (We Hope)</h3> | ||
<p>After testing and verifying these parts, we will compare the ethanol production in wild-type yeast and yeast with the light-sensitive switch. Wild-type strains face certain limitations in their ethanol production; they tend to immediately ferment large quantities of fermentable carbohydrates, limiting their colony size and therefore output. They also metabolize the ethanol they create, lowering yield. By forcing our yeast to respire aerobically at the beginning, we should be able to grow a larger colony. Then we will force the larger-than-wild-type colony to ferment their food source into ethanol, and ensure they cannot metabolize that ethanol. The end result, we expect, will be more ethanol in less time.</p> | <p>After testing and verifying these parts, we will compare the ethanol production in wild-type yeast and yeast with the light-sensitive switch. Wild-type strains face certain limitations in their ethanol production; they tend to immediately ferment large quantities of fermentable carbohydrates, limiting their colony size and therefore output. They also metabolize the ethanol they create, lowering yield. By forcing our yeast to respire aerobically at the beginning, we should be able to grow a larger colony. Then we will force the larger-than-wild-type colony to ferment their food source into ethanol, and ensure they cannot metabolize that ethanol. The end result, we expect, will be more ethanol in less time.</p> | ||
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Revision as of 19:03, 30 June 2016
Mito-Switch Project Description
General Overview
As an extension of Team RHIT’s 2015 iGEM project, the end goal of our project is to control aerobic and anaerobic respiration in yeast in order to optimize ethanol production. To this effect, we intend to manipulate the expression of a ribosomal protein coded for by the gene mRPS12 in the mitochondria of the yeast species Saccharomyces cerevisiae. While there may be more direct means of regulation (e.g., by enzymes ADH1 and ADH2), this project method will build a foundation for the potential optimization of production of other secondary metabolites.
Basic Trajectory and Parts
As an extension of 2015 RHIT iGEM project, the first portion of the project this year is to verify the function of the two parts submitted last year. These parts are the mRPS12 translational unit and the mRPS12 mitochondrial localization signal. We will start things of by creating yeast vectors that are compatible with the BioBrick standard prefix and suffix. These modified standard vectors will allow an easier clone of parts into yeast cells in our project, and other iGEM teams will be able to use them for similar practices in the future as well. The function of mls will be verified by linking it to GFP. The function of mRPS12 will be verified by transforming mRPS12 knock out yeast and testing for restored mitochondrial function.
The second portion of this project consists of incorporating a red light sensitive switch into S. cerevisiae to enable yeast respiration control. We will verify LexA light switch system consisting of parts BBa_K801039, BBa_K801041, and a LexA promoter.
Results (We Hope)
After testing and verifying these parts, we will compare the ethanol production in wild-type yeast and yeast with the light-sensitive switch. Wild-type strains face certain limitations in their ethanol production; they tend to immediately ferment large quantities of fermentable carbohydrates, limiting their colony size and therefore output. They also metabolize the ethanol they create, lowering yield. By forcing our yeast to respire aerobically at the beginning, we should be able to grow a larger colony. Then we will force the larger-than-wild-type colony to ferment their food source into ethanol, and ensure they cannot metabolize that ethanol. The end result, we expect, will be more ethanol in less time.
