Difference between revisions of "Team:CSU Fort Collins/NoteBook/JanThroughApr"

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<p>Ran PCR results on 1% gel</p>
 
<p>Ran PCR results on 1% gel</p>
 
<img src = "https://static.igem.org/mediawiki/2016/c/c0/CSU_gJan16Slr0435.png">
 
<img src = "https://static.igem.org/mediawiki/2016/c/c0/CSU_gJan16Slr0435.png">
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<div class = 'Days'><h5>2/6/16:</h5></div>
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<div class = 'Info'><p>PCRed out BBA_J23106 out of glycerol stock 19 using Q5 master mix 19rxns</p>
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</div>
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<div class = 'Days'><h5>2/9/16:</h5></div>
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<div class = 'Info'><p>Transformed Bba_K325909 from parts kit into DH5a cels</p>
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</div>
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<div class = 'Days'><h5>2/11/16:</h5></div>
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<div class = 'Info'><p>To confirm that Bba_K325909 are functioning we tried to induce expression using L-arabinose</p>
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<p>No luminescence was produced</p>
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</div>
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<div class = 'Days'><h5>3/24/16:</h5></div>
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<div class = 'Info'><p>Set up O/N cultures of K1033929, K592009 and E1010 in 5 mL of LB with 5 uL of Cm34 antibiotic </p>
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</div>
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<div class = 'Days'><h5>3/29/16:</h5></div>
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<div class = 'Info'><p>Ran promoters Sll027, and LrtA in a 1% gel</p>
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<h6>Sll0027</h6>
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<img src = "https://static.igem.org/mediawiki/2016/0/0f/CSU_gSll02632416.png">
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<h6>LrtA</h6>
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<img src = "https://static.igem.org/mediawiki/2016/3/33/CSU_gLrta32416.png">
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Revision as of 21:36, 16 October 2016

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CSU iGEM



January Through April

1/11/16:

slr0435 Excision from Synechocystis sp. PCC6803 with PCR using Phusion(size 276 bp).

Ran PCR results on a 2% gel

1/12/16:

Digested slr0435 and resuspended pSB1C3 with EcoRI and PstI

Ligated pSB1C3 and slr0435

Transformed ligation mixture

1/13/16:

Colony PCR performed on slr0435+pSB1C3 transformed colonies

Ran PCR results on 1% gel

2/6/16:

PCRed out BBA_J23106 out of glycerol stock 19 using Q5 master mix 19rxns

2/9/16:

Transformed Bba_K325909 from parts kit into DH5a cels

2/11/16:

To confirm that Bba_K325909 are functioning we tried to induce expression using L-arabinose

No luminescence was produced

3/24/16:

Set up O/N cultures of K1033929, K592009 and E1010 in 5 mL of LB with 5 uL of Cm34 antibiotic

3/29/16:

Ran promoters Sll027, and LrtA in a 1% gel

Sll0027
LrtA