Difference between revisions of "Team:Cambridge-JIC/Attributions"
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<li><b>Dr. Nicola Patron</b>, Earlham Institute, for helping us with define our Cas9 strategy, and practical advice on the using CRISPR-Cas9 safely | <li><b>Dr. Nicola Patron</b>, Earlham Institute, for helping us with define our Cas9 strategy, and practical advice on the using CRISPR-Cas9 safely | ||
<li><b>Dr. Sarah Richardson</b>, Ignition Genomics, for discussing our project with us. | <li><b>Dr. Sarah Richardson</b>, Ignition Genomics, for discussing our project with us. | ||
| − | <li><b>Dr. Jim Ajioka</b>, University of Cambridge Department of Pathology, for discussing our project with us. | + | <li><b>Dr. Jim Ajioka</b>, University of Cambridge, Department of Pathology, for discussing our project with us. |
<li><b>Prof. Tom Knight</b>, Ginkgo Bioworks, for discussing our project with us. | <li><b>Prof. Tom Knight</b>, Ginkgo Bioworks, for discussing our project with us. | ||
</ul> | </ul> | ||
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</div> | </div> | ||
<div class="container"> | <div class="container"> | ||
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<hr> | <hr> | ||
| + | <h2 style="font-family:Montserrat ; text-align: center">WETLAB</h2> | ||
| + | <h4 style="font-family:Roboto; font-weight:bold; text-align: center"><em>University of Cambridge, Department of Plant Sciences</em></h4> | ||
| + | <ul style="text-align:left; float:left; font-size:120%"> | ||
| + | <li><b>Dr. Moritz Meyer</b>, for teaching us how to make TAP growth medium, culture Chlamydomonas reinhardtii, amplify DNA plasmids in bacteria and extract them, biolistics and for providing some consumables, especially for biolistics. | ||
| + | <li><b>Dr Payam Mehrshahi</b> and <b>Mr. Aleix Gorchs-Rivera</b>, for all the help and advice on the molecular cloning strategies and analysis of results. | ||
| + | <li><b>Dr. Farhat Nazir</b>, for laboratory supervision in the second half of our project, explaining how to use devices and what to be cautious about in lab procedures | ||
| + | <li><b>Ms. Barbara Landamore</b>, for giving a safety training, providing standard laboratory equipment and help with technical issues and organisation in the lab | ||
| + | <li><b>Ms. Linda Silvestri</b>, for helping us manage our lab consumables and hardware part orders, funds and donating her stocks of competent cells | ||
| + | <li><b>Ms. Gabriela Doria</b>, for helping us to run a few experiments | ||
| + | <li><b>Dr. Katrin Geisler</b>, helped us with the biolistics procedure | ||
| + | </ul> | ||
</div> | </div> | ||
</section> | </section> | ||
Revision as of 21:43, 16 October 2016
ATTRIBUTIONS
Our project could not have existed without the following people, and we are eternally grateful for their contributions.
“TOOLBOX” FORMULATION
University of Cambridge, Department of Plant Sciences
- Prof. Jim Haseloff, for giving us our one week introductory course to iGEM and synthetic biology, and continuous general advice
- Dr. Susana Sauret-Gueto, for guidance in our experimental design and great advice on how to manage and arrange a lab for maximum efficiency. Also for regularly checking on our work and giving very useful feedback.
- Dr. Moritz Meyer, for his help and support in the lab, not just with practical advice but with his constant sense of humour and unlimited patience.
- Mr. Aleix Gorchs-Rovira, for always being able to answer our endless questions on PCR and cloning, as well as help with the golden gate design (another example of endless patience)
- Dr. Payam Mehrshahi, for his always kind words, interest in the project development and help with protocols and analysis of results.
- Dr. Doris Gangl, for advice on strain selection with regards to biological containment, and working with Chlamydomonas.
- Dr. Francisco Navarro, for giving us insight on the measurement of fluorescence in Chlamydomonas reinhardtii.
Other sources of help
- Prof. Saul Purton, University College London, for providing us a series of chloroplast transformation vectors as well as the TN72 Chlamydomonas reinhardtii strain, also for the help in wetlab with protocols and analysis of molecular cloning with Chlamydomonas.
- Mr. Marco Larrea Alvarez, University College London, group member of Paul Purton’s lab for advice on working with Chlamydomonas as well as inviting us to UCL to troubleshoot some difficulties with the system and give us feedback of what parts would be useful to the research community.
- Dr. Nicola Patron, Earlham Institute, for helping us with define our Cas9 strategy, and practical advice on the using CRISPR-Cas9 safely
- Dr. Sarah Richardson, Ignition Genomics, for discussing our project with us.
- Dr. Jim Ajioka, University of Cambridge, Department of Pathology, for discussing our project with us.
- Prof. Tom Knight, Ginkgo Bioworks, for discussing our project with us.
WETLAB
University of Cambridge, Department of Plant Sciences
- Dr. Moritz Meyer, for teaching us how to make TAP growth medium, culture Chlamydomonas reinhardtii, amplify DNA plasmids in bacteria and extract them, biolistics and for providing some consumables, especially for biolistics.
- Dr Payam Mehrshahi and Mr. Aleix Gorchs-Rivera, for all the help and advice on the molecular cloning strategies and analysis of results.
- Dr. Farhat Nazir, for laboratory supervision in the second half of our project, explaining how to use devices and what to be cautious about in lab procedures
- Ms. Barbara Landamore, for giving a safety training, providing standard laboratory equipment and help with technical issues and organisation in the lab
- Ms. Linda Silvestri, for helping us manage our lab consumables and hardware part orders, funds and donating her stocks of competent cells
- Ms. Gabriela Doria, for helping us to run a few experiments
- Dr. Katrin Geisler, helped us with the biolistics procedure
