Difference between revisions of "Team:Tongji Shanghai/Description"

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            <a class="navbar-brand" href="https://2016.igem.org/Team:Tongji_Shanghai"><img alt="" src="images/margo.png"></a>
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            <ul class="nav navbar-nav navbar-right">
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              <li>
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                <a href="#">Achievement</a>
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                <ul class="dropdown">
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                  <li><a href="contact.html">Judging Criteria</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Parts">Parts</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Model">Modeling</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Notebook">Notebook</a></li>
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                </ul>
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            </li> 
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              <li>
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                <a href="#">Project</a>
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                <ul class="dropdown">
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Description">Descrpition</a></li>
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                  <li><a href="#">Topic 1</a></li>
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                  <li><a href="#">Topic 2</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Experiments">Experiments</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Results">Results</a></li>
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                </ul>
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              </li>
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              <li>
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                <a href="#">Outreach</a>
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                <ul class="dropdown">
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Human_Practices">Human Practice</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Collaborations">Collaboration</a></li>
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                </ul>
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              </li>               
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              <li>
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                <a href="#">Lab</a>
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                <ul class="dropdown">
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Team">Team</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Attributions">Attribution</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/About">About</a></li>
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                </ul>
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              </li> 
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              <li>
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                <a href="https://2016.igem.org/Team:Tongji_Shanghai/Safety">Safety</a>
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                <ul class="dropdown">
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Safety">Lab Safety</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Safety">Bio Safety</a></li>
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                </ul>
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        <ul class="wpb-mobile-menu">
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          <li>
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                <a href="#">Achievement</a>
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                <ul class="dropdown">
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                  <li><a href="contact.html">Judging Criteria</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Parts">Parts</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Model">Modeling</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Notebook">Notebook</a></li>
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                </ul>
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          </li>
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          <li>
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                <a href="#">Project</a>
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                <ul class="dropdown">
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Description">Descrpition</a></li>
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                  <li><a href="#">Topic 1</a></li>
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                  <li><a href="#">Topic 2</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Experiments">Experiments</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Results">Results</a></li>
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                </ul>
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          </li>
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          <li>
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                <a href="#">Outreach</a>
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                <ul class="dropdown">
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Human_Practices">Human Practice</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Collaborations">Collaboration</a></li>
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                </ul>
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          </li>
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          <li>
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                <a href="#">Lab</a>
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                <ul class="dropdown">
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Team">Team</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Attributions">Attribution</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/About">About</a></li>
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                </ul>
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          </li>
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          <li>
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                <a href="https://2016.igem.org/Team:Tongji_Shanghai/Safety">Safety</a>
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                <ul class="dropdown">
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Safety">Lab Safety</a></li>
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                  <li><a href="https://2016.igem.org/Team:Tongji_Shanghai/Safety">Bio Safety</a></li>
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                </ul>           
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          </li>
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          <li>
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            <a href="contact.html">Contact</a>
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             <div class="widget widget-categories" style="display: block;position: fixed;">
 
               <h4>Description <span class="head-line"></span></h4>
 
               <h4>Description <span class="head-line"></span></h4>
 
               <ul>
 
               <ul>
 
                 <li>
 
                 <li>
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                   <a href="#">Topic 1</a>
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                   <a href="#">Materials Synthesis</a>
 
                 </li>
 
                 </li>
 
                 <li>
 
                 <li>
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                   <a href="#">Topic 2</a>
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                   <a href="#">Plasmid Construction</a>
 
                 </li>
 
                 </li>
 
                 <li>
 
                 <li>
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                   <a href="#">Topic 3</a>
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                   <a href="#">Cell Experiments</a>
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                </li>
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                <li>
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                  <a href="#">Mice Experiments</a>
 
                 </li>
 
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              <h3 class="classic-title" style="margin-top:0px"><span><a  href="https://2015.igem.org/Team:Peking/Design/PC_Reporter">Paired dCas9 reporter</a></span></h3>
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                <p> It is well known that CRISPR/dCas9 has a unique ability to be programmed to bind any sequence with the assistance of sgRNA; it was conventionally used for DNA editing or genome study. In our project, however, we integrate split reporters into CRISPR/Cas9 by translationally fusing two fragments of a split reporter to dCas9, respectively, to convert the sequence-specific information of pathogenic bacteria's genome (in our case, <i>M. tuberculosis</i>) into easily readable signal including bioluminescence or pigment. We demonstrated that the PC reporter is highly compatible with NAD-based diagnosis using isolated genomic DNA of MTB.</p>
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              <h3 class="classic-title" style="margin-top:0px"><span><a  href="https://2015.igem.org/Team:Peking/Design/PC_Reporter">Paired dCas9 reporter</a></span></h3>
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        <!-- Materials Synthesis -->  
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      <h3 class="classic-title" style="margin-top:0px"><span>Materials Synthesis</span></h3>
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                <p> It is well known that CRISPR/dCas9 has a unique ability to be programmed to bind any sequence with the assistance of sgRNA; it was conventionally used for DNA editing or genome study. In our project, however, we integrate split reporters into CRISPR/Cas9 by translationally fusing two fragments of a split reporter to dCas9, respectively, to convert the sequence-specific information of pathogenic bacteria's genome (in our case, <i>M. tuberculosis</i>) into easily readable signal including bioluminescence or pigment. We demonstrated that the PC reporter is highly compatible with NAD-based diagnosis using isolated genomic DNA of MTB.</p>
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            <ul style="margin-left:0px; font-size:16px">We have synthesized the AuNRs (gold nanorods) and detoxified them for photothermal therapy, also known as optical hyperthermia or photothermal ablation, which is an emerging strategy for treating solid tumors. Gold nanoparticles are capable of confining resonant photons, further inducing coherent surface plasmon oscillation of their conduction band electrons.<br><br>
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            For non-invasive therapy, near infrared (NIR) radiation is chosen because it penetrates tissue more deeply. And we find that AuNRs with a strong SPR(surface plasmon resonance) in the NIR region can show intense absorption of light in the NIR region, also biocompatibility. Even more, it can accumulate in tumor tissue via passive targeting phenomena. PEG can be used to increase biocompatibility, suppress immunogenic responses and decrease adsorption. </ul>
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        <!-- Cell Experiments-->
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            <h3 class="classic-title" style="margin-top:20px"><span>Cell Experiments</span></h3> 
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          <!-- Some Text -->
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            <ul style="margin-left:0px; font-size:16px">In order to test the toxicity and killing efficiency of our system, we have designed in vitro cytotoxicity test and in vitro photo thermal effect test. Cell viability is tested with “cell Titer-Glo luminescent cell viability assay”, which reflects the ATP concentration of cells, and also “cell apoptosis kit”, which reflects percentage of living cells.
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                  <img style="width:100%;" alt="" src="https://static.igem.org/mediawiki/2016/d/d1/T--Tongji_Shanghai--TEAM_GROUP_1.jpg">
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                  <div class="item">
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                  <img style="width:100%;" alt="" src="https://static.igem.org/mediawiki/2016/b/bd/T--Tongji_Shanghai--TEAM_GROUP_3.jpg">
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                    <span class="glyphicon" aria-hidden="true"><img style="margin-top:700%" src="https://static.igem.org/mediawiki/2015/d/d1/Peking-left.png"></span>
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                    <span class="sr-only">Previous</span>
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            <ul style="margin-left:0px; font-size:16px;">We have synthesized the AuNRs (gold nanorods) and detoxified them for photothermal therapy, also known as optical hyperthermia or photothermal ablation, which is an emerging strategy for treating solid tumors. Gold nanoparticles are capable of confining resonant photons, further inducing coherent surface plasmon oscillation of their conduction band electrons.<br><br>
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            For non-invasive therapy, near infrared (NIR) radiation is chosen because it penetrates tissue more deeply. And we find that AuNRs with a strong SPR(surface plasmon resonance) in the NIR region can show intense absorption of light in the NIR region, also biocompatibility. Even more, it can accumulate in tumor tissue via passive targeting phenomena. PEG can be used to increase biocompatibility, suppress immunogenic responses and decrease adsorption. </ul>
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Revision as of 11:11, 17 October 2016

Tongji_Shanghai-2016.igem.org Tongji Shanghai

Project

Our project is our story.

Description

Materials Synthesis

    We have synthesized the AuNRs (gold nanorods) and detoxified them for photothermal therapy, also known as optical hyperthermia or photothermal ablation, which is an emerging strategy for treating solid tumors. Gold nanoparticles are capable of confining resonant photons, further inducing coherent surface plasmon oscillation of their conduction band electrons.

    For non-invasive therapy, near infrared (NIR) radiation is chosen because it penetrates tissue more deeply. And we find that AuNRs with a strong SPR(surface plasmon resonance) in the NIR region can show intense absorption of light in the NIR region, also biocompatibility. Even more, it can accumulate in tumor tissue via passive targeting phenomena. PEG can be used to increase biocompatibility, suppress immunogenic responses and decrease adsorption.


Cell Experiments

    In order to test the toxicity and killing efficiency of our system, we have designed in vitro cytotoxicity test and in vitro photo thermal effect test. Cell viability is tested with “cell Titer-Glo luminescent cell viability assay”, which reflects the ATP concentration of cells, and also “cell apoptosis kit”, which reflects percentage of living cells.


Materials Synthesis

    We have synthesized the AuNRs (gold nanorods) and detoxified them for photothermal therapy, also known as optical hyperthermia or photothermal ablation, which is an emerging strategy for treating solid tumors. Gold nanoparticles are capable of confining resonant photons, further inducing coherent surface plasmon oscillation of their conduction band electrons.

    For non-invasive therapy, near infrared (NIR) radiation is chosen because it penetrates tissue more deeply. And we find that AuNRs with a strong SPR(surface plasmon resonance) in the NIR region can show intense absorption of light in the NIR region, also biocompatibility. Even more, it can accumulate in tumor tissue via passive targeting phenomena. PEG can be used to increase biocompatibility, suppress immunogenic responses and decrease adsorption.


Mice Experiments

    Previous work has shown that golden nanorods is bio-safe to cells under 100μg/ml, and perform well when exposed to 820nm NIR. In mice experiments, we use lentivirus to transport plasmid into tumor. The feasibility of in vivo near-infrared OPTT is demonstrated after infecting lentivirus in tumor-bearing mice by direct injection. A portable wave diode laser is applied to cooperate with this therapy.