It has been a summer of a lot of work, many mistakes and many corrections. Below you will find a bullet points of the broad overview of the successes and less successful aspects of our wetlab project. We then elaborate in text our experience and where we put our effort in. We have included “failures” and “mistakes” as we consider these to be useful to future iGEM teams who brave the challenge of working with plastids!

What we achieved this summer

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We assisted by providing Imperial iGEM with growth data from Chlamydomonas, for their computer model A.L.I.C.E. This computer model is aimed at producing a platform with the growth conditions for various different species, and ultimately for identifying possible co-cultures. Find out more here

We hope that the data we provide is especially useful for Imperial iGEM, as chlamydomonas takes significantly longer to grow that bacterial species.

Linköping University

The collaborations with Linköping University has been very helpful for both parties as we exchanged weekly reports through emails and Skype meetings. In depth discussions on the chasis we were working on, Chlamydomonas reinhardtii, were carried out. In addition, we exchanged views on Gibson assembly and other higher level assembly methods' protocols to help each other make an informed decision on the best approach for our project.