Line 58: | Line 58: | ||
<section style="background-color:#3d3d3d; text-align: center; padding: 15% 0%"> | <section style="background-color:#3d3d3d; text-align: center; padding: 15% 0%"> | ||
<div class="container" style="color:#fff"> | <div class="container" style="color:#fff"> | ||
− | <p class="darkBlue" style="font-family:Open Sans; font-size: | + | <p class="darkBlue" style="font-family:Open Sans; font-size:180%; text-align:center">I. Microalgal chlroplasts as an alternative expression system</p> |
</div> | </div> | ||
</section> | </section> | ||
Line 100: | Line 100: | ||
<section style="background-color:#fff; text-align: center; padding: 15% 0%"> | <section style="background-color:#fff; text-align: center; padding: 15% 0%"> | ||
<div class="container" style="color:#000"> | <div class="container" style="color:#000"> | ||
− | <p style="font-family:Open Sans; font-size: | + | <p style="font-family:Open Sans; font-size:180%; text-align:center">II. Microalgae chloroplasts as a model for higher plants</p> |
</div> | </div> | ||
</section> | </section> |
Revision as of 17:10, 18 October 2016
![](http://images.clipartpanda.com/gear-clipart-9T4eppGbc.png)
OUR MOTIVATION...
I. Microalgal chlroplasts as an alternative expression system
Chloroplasts hold a massive potential as an alternative protein expression system, due to their outstanding expression yields, diversity of post-translational modifications and auto/mixotrophic lifestyles of plants and microalgae.
![](https://static.igem.org/mediawiki/2016/8/8c/T--Cambridge-JIC--motivation--table1.jpg)
Various proteins have already been successfully expressed in chloroplasts, including:
- monoclonal antibodies
- antigens
- anti-toxins
- growth factors
Transgene expression in microalgae can total up to 30-50% of a cell’s dry biomass, as unlike in higher plants and mammals, metabolic energy in microalgae is not directed towards maintaining complex differentiated structures.
II. Microalgae chloroplasts as a model for higher plants
I. Microalgal chlroplasts as an alternative expression system