Difference between revisions of "Team:TJUSLS China/Safety"

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                <li onmouseover="getHover(0)"><a href="https://2016.igem.org/Team:TJUSLS_China">TJUSLS</a></li>
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                <li onmouseover="getHover(1)" class=""><a href="" class="root">project</a>
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                    <ul>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Description">Description</a></li>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Design">Design</a></li>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Experiments">Experiments</a></li>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Proof">Proof of Concept</a></li>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Demonstrate">Demonstrate</a></li>
  
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Notebook">Notebook</a></li>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/PROJECT/PROTOCOLS">Protocols</a></li>
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                    </ul>
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                </li>
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                <li onmouseover="getHover(2)" class=""><a href="https://2016.igem.org/Team:TJUSLS_China/Parts">PARTS</a>
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                    <ul>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Basic_Part">Basic Parts</a></li>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Composite_Part">Composite Parts</a></li>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Part_Collection">Part Collection</a></li>
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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                    </ul>
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                </li>
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                <li class="active" onmouseover="getHover(3)"><a class="active" href="https://2016.igem.org/Team:TJUSLS_China/Safety">SAFETY</a></li>
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                <li onmouseover="getHover(4)" class=""><a href="https://2016.igem.org/Team:TJUSLS_China/Attributions">ATTRIBUTIONS</a>
  
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</div>
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                </li>
  
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                <li onmouseover="getHover(5)" class=""><a href="https://2016.igem.org/Team:TJUSLS_China/Human_Practices">HUMAN PRACTICES</a>
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                    <ul>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/HP/Silver">Silver</a></li>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/HP/Gold">Gold</a></li>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Integrated_Practices">Integrated Practices</a></li>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Engagement">Engagement</a></li>
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                    </ul>
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                </li>
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                <li onmouseover="getHover(6)" class=""><a href="https://2016.igem.org/Team:TJUSLS_China/Modeling">MODELING</a>
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                    <ul>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Modeling">Modeling</a></li>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Software">Software</a></li>
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                    </ul>
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                </li>
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                <li onmouseover="getHover(7)" class=""><a href="https://2016.igem.org/Team:TJUSLS_China/Team">TEAM</a>
  
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                    <ul>
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<h5>Safe Project Design</h5>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Team">Team</a></li>
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                        <li><a href="https://2016.igem.org/Team:TJUSLS_China/Collaborations">Collaborations</a></li>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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                    </ul>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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    <div class="sec-menu">
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<img class="sec-menu-close" src="https://static.igem.org/mediawiki/2016/b/b5/T--TJUSLS_China--sec-menu-close.png"/>
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        <ul>
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            <li><a href="#part1">Project Design</a></li>
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            <li><a href="#part2">Lab Work</a></li>
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            <li><a href="#part3">Shipment</a></li>
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<h5>Safe Lab Work</h5>
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    Safety
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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        <img src="https://static.igem.org/mediawiki/2016/8/86/T--TJUSLS_China--second-logo.png"/>
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<h5>Safe Shipment</h5>
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        <h3 id="part1">Project Design</h3>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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        <div class="sec-wenzi-list">1.  PETase is nontoxic</div>
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        <div class="sec-wenzi-content"> Our project is on the basis of PETase and its respective mutants.PETase is isolated from a novel bacterium,Ideonella sakaiensis 201-F6, that is able to use PET as its major energy and carbon source. Thus, we have good reasons to believe there isn't any safety problem about PETase.</div>
 +
        <div class="sec-wenzi-list">2. Our new applications are more environment-friendly</div>
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        <div class="sec-wenzi-content">About our Trainning Protein project, there is no doubt that it is environment-friendly. It not only helps to solve the plastic pollution problem, using the ezymolysis way not just landfill, incineration and chemical decomposition, but also enhances its effect.</div>
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        <div class="clear"></div>
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    <div class="sec-list-wenzi">
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        <h3 id="part2">Lab Work</h3>
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        <div class="sec-wenzi-content">In order to avoid danger and train our team member, everyone accepted the biological safety test even some of our members in charge of wiki. Of course, they all qualified. What’s more, when we formally conducted experiments, every member will be supervised by our instructors and each other. If we found who conducted work nonstandardly, we will punish him/her by being on duty at that day. And all our waste are discarded safely, strictly followed stipulations of our school.</div>
 +
        <div class="sec-wenzi-content">We chose BL21 (DE3) and Pichia Pastoris as our expressing strain, and we first constructed expression vectors in E. coli DH5alpha. In the process, use kits which are safe to extract, construct and transform our plasmids. ALL THE ORGANISM we use in our lab are safety level 1 organisms. We conducted our operations on open clean benches and all the waste contains microorganisms are sterilized before further dispose to ensure safety. According to the situation, we will choose to wear protective equipment.</div>
 +
        <div class="sec-wenzi-ul">
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            <div class="wenzi-dt">And the following is our regulations of lab:</div>
 +
            <div class="wenzi-li">Be sure that you have worn protective equipment if necessary.</div>
 +
            <div class="wenzi-li">Be sure that you have informed the instructor of the next step of the experiment plan and gotten the permission of him.</div>
 +
            <div class="wenzi-li">Keep the super clean bench clean and operate correctly.</div>
 +
            <div class="wenzi-li">Properly handle the waste generated in the experiment.</div>
 +
            <div class="wenzi-li">Clean the table after the experiment.</div>
 +
            <div class="wenzi-li">Make the experiment record and finish experiment results in time.</div>
 +
        </div>
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    <div class="sec-list-wenzi">
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        <h3 id="part3">Shipment</h3>
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        <div class="sec-wenzi-content">To achieve safe shipment, we followed the iGEM official advice for Teams in China to send the samples to GenScript in Nanjin,China which is quick and convenient.</div>
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Latest revision as of 05:09, 19 October 2016

Menu
Safety

Project Design

1. PETase is nontoxic
Our project is on the basis of PETase and its respective mutants.PETase is isolated from a novel bacterium,Ideonella sakaiensis 201-F6, that is able to use PET as its major energy and carbon source. Thus, we have good reasons to believe there isn't any safety problem about PETase.
2. Our new applications are more environment-friendly
About our Trainning Protein project, there is no doubt that it is environment-friendly. It not only helps to solve the plastic pollution problem, using the ezymolysis way not just landfill, incineration and chemical decomposition, but also enhances its effect.

Lab Work

In order to avoid danger and train our team member, everyone accepted the biological safety test even some of our members in charge of wiki. Of course, they all qualified. What’s more, when we formally conducted experiments, every member will be supervised by our instructors and each other. If we found who conducted work nonstandardly, we will punish him/her by being on duty at that day. And all our waste are discarded safely, strictly followed stipulations of our school.
We chose BL21 (DE3) and Pichia Pastoris as our expressing strain, and we first constructed expression vectors in E. coli DH5alpha. In the process, use kits which are safe to extract, construct and transform our plasmids. ALL THE ORGANISM we use in our lab are safety level 1 organisms. We conducted our operations on open clean benches and all the waste contains microorganisms are sterilized before further dispose to ensure safety. According to the situation, we will choose to wear protective equipment.
And the following is our regulations of lab:
Be sure that you have worn protective equipment if necessary.
Be sure that you have informed the instructor of the next step of the experiment plan and gotten the permission of him.
Keep the super clean bench clean and operate correctly.
Properly handle the waste generated in the experiment.
Clean the table after the experiment.
Make the experiment record and finish experiment results in time.

Shipment

To achieve safe shipment, we followed the iGEM official advice for Teams in China to send the samples to GenScript in Nanjin,China which is quick and convenient.

Copyright 2013 TJUSLS. Template by  W3Layouts