Difference between revisions of "Team:Stony Brook/Notebook"

 
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<p> Document the dates you worked on your project.</p>
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<h1> Lab Notebook </h1>
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<h5>What should this page have?</h5>
 
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<ul>
 
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<li>Chronological notes of what your team is doing.</li>
 
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<li> Brief descriptions of daily important events.</li>
 
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<li>Pictures of your progress. </li>
 
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<li>Mention who participated in what task.</li>
 
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</ul>
 
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<h5>Inspiration</h5>
 
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<p>You can see what others teams have done to organize their notes:</p>
 
  
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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<h1> <a id="stuff" href="https://2016.igem.org/Team:Stony_Brook/Notebook/Cancer-W1">Week 1: 6/28 - 7/1 </a> </h1>
  
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<h2> Week 1 (6/27 - 7/1) </h2>
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<h1> <a id="stuff" href="https://2016.igem.org/Team:Stony_Brook/Notebook/Cancer-W2">Week 2: 7/4 - 7/8 </a> </h1>
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<h4> 6/27 </h4>
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<h5> Cancer Group </h5>
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<h1> <a id="stuff" href="https://2016.igem.org/Team:Stony_Brook/Notebook/Cancer-W3">Week 3: 7/11 - 7/17 </a> </h1>
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<ul>
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<li> PCR of 3xHA-TDGF1 construct </li>
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<li> Gel of PCR Product → PCR was a failure </li>
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<li> Second PCR of 3xHA-TDGF1 construct → left overnight </li>
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</ul>
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<h5> Interlab Study </h5>
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<h1> <a id="stuff" href="https://2016.igem.org/Team:Stony_Brook/Notebook/Cancer-W4">Week 4: 7/18 - 7/24</a></h1>
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<li> Measured LUDOX and water for plate reader </li>
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<li> Transformation of positive and negative controls, test devices 1-3 </li>
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<h4> 6/28</h4>
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<h1> <a id="stuff" href="https://2016.igem.org/Team:Stony_Brook/Notebook/Cancer-W5">Week 5: 7/25 - 7/31</a> </h1>
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<h5> Cancer Group </h5>
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<h1> <a id="stuff" href="https://2016.igem.org/Team:Stony_Brook/Notebook/Cancer-W6">Week 6: 8/1 - 8/7</a></h1>
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<ul>
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<li> Ran gel on PCR of yesterday's construct → Gel worked </li>
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<li> Gel purification of PCR Product → low concentration of DNA found during nanodrop</li>
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<li> PCR the construct using a revised method </li>
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<li> 2:54pm → ran the gel on the PCR product </li>
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</ul>
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<h5> Interab Study </h5>
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<h1> <a id="stuff" href="https://2016.igem.org/Team:Stony_Brook/Notebook/Cancer-W7">Week 7: 8/8 - 8/14</a></h1>
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<ul>
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<li> Heat and "SB" streaking seems not to have worked</li>
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<li> No visible red colonies on plate </li>
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<h4> 6/29 </h4>
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<h1> <a id="stuff"href="https://2016.igem.org/Team:Stony_Brook/Notebook/Cancer-W8">Week 8: 8/15 - 8/21</a> </h1>
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<h5> Cancer Group </h5>
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<h1> <a id="stuff" href="https://2016.igem.org/Team:Stony_Brook/Notebook/Cancer-W9">Week 9: 8/22 - 8/28</a></h1>
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<ul>
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<li> PCR Not working well → keep trying new methods with different annealing temperatures </li>
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<li> Tried 65 and 67 degrees C</li>
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<h5> Interlab Study </h5>
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<h1> <a id="stuff" href="https://2016.igem.org/Team:Stony_Brook/Notebook/Cancer-W10"> Week 10: 8/29 - 9/4 </a> </h1>
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<ul>
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<li> FITC started </li>
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<li> Cutting re-inoculated </li>
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<li> Ordered new LUDOX </li>
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<h5> Other </h5>
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<h1> <a id="stuff" href="https://2016.igem.org/Team:Stony_Brook/Notebook/Cancer-W11"> Week 11: 9/5 - 9/11 </a> </h1>  
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<ul>
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<li> College of Arts and Sciences Pre College Summer Institute students stopped by lab </li>
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<li> Spoke to them about information on synthetic biology and iGEM </li>  
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<h4> 6/30 </h4>
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<h1> <a id="stuff" href="https://2016.igem.org/Team:Stony_Brook/Notebook/Cancer-W14"> Week 12: 9/26 - 10/2 </a> </h1>
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<h5> Cancer Group </h5>
 
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<li> PCR worked → Test 5 → changed annealing temperature to 66 degrees C + increased the denaturing time </li>
 
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<li> Nanodropped DNA</li>
 
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<h4> 7/1 </h4>
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<h5> Cancer Group </h5>
 
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<li> Digestion of TDGF-1 construct and YFP352GAP vector </li>
 
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<p> TDGF1 Construct </p>
 
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Latest revision as of 07:53, 19 October 2016

Lab Notebook