Difference between revisions of "Team:CSU Fort Collins/NoteBook/July"

 
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<h1 align = "center">July</h1>
 
<h1 align = "center">July</h1>
  
 +
<div class = 'Days'><h5>7/2/16:</h5></div>
 +
<div class = 'Info'><ul><li>Ran PCR products from inter lab study colony PCR on a 1% gel</li>
 +
<li>No products</li>
 +
</ul>
 +
</div>
  
 
<div class = 'Days'><h5>7/6/16:</h5></div>
 
<div class = 'Days'><h5>7/6/16:</h5></div>
 
<div class = 'Info'><ul><li>PCRed out Lrta from <i>Synechocystis PCC 6003</i></li>
 
<div class = 'Info'><ul><li>PCRed out Lrta from <i>Synechocystis PCC 6003</i></li>
−
 
+
<li>Re ran gel from inter lab study colony PCR on 1% gel</li>
−
<img src ="https://static.igem.org/mediawiki/2016/2/2a/CSU_Slr01685116.png">
+
<li>No products</li>
 
</ul>
 
</ul>
 
</div>
 
</div>
Line 41: Line 58:
 
</div>
 
</div>
  
 +
<div class = 'Days'><h5>7/8/16:</h5></div>
 +
<div class = 'Info'><ul><li>Performed ligase cycling reaction on slr0168 and pSB1C3</li>
 +
<li>Ran all PCR products on 1%gel</li>
 +
<img src ="https://static.igem.org/mediawiki/2016/f/fe/CSU_SLR01687716.png">
 +
<li>Digested Lrta(SpeI EcorI)</li>
 +
<li>Ligated Lrta and EYFP-LVA</li>
 +
<li>Transformed Lrta+EYFP-LVA ligation mixture</li>
 +
</ul>
 +
</div>
  
 +
<div class = 'Days'><h5>7/12/16:</h5></div>
 +
<div class = 'Info'><ul><li>Dpn1 Digested slr0168  and pSB1C3</li>
 +
<li>Phosphorylated slr0168 and pSB1C3</li>
 +
<li>Performed ligase cycling reaction on phosphorylated mixture</li>
 +
<li>Transformed ligase cycling reaction products</li>
 +
<li>Digested EYFP-LVA(PstI)</li>
 +
<li>Ligated EYFP-LVA + LrtA</li>
 +
<li>Transformed EYFP-LVA+LrtA ligation mixture</li>
 +
<li>Performed colony PCR on 13 colonies with the Q1 block</li>
 +
<li>Ran PCR results on a 1.5% gel</li>
 +
<img src="https://static.igem.org/mediawiki/2016/e/e8/CSU_Q171216.png">
 +
</ul>
 +
</div>
  
 +
<div class = 'Days'><h5>7/13/16:</h5></div>
 +
<div class = 'Info'><ul><li>Colony PCR performed on 10 EYFP-LVA+LrtA transformed colonies</li>
 +
<li>PCR amplified Q1 and Q2 for infusion cloning</li>
 +
</ul>
 
</div>
 
</div>
 +
 +
<div class = 'Days'><h5>7/14/16:</h5></div>
 +
<div class = 'Info'><ul><li>Ran Q1 and Q2 blocks in a 1.5% gel</li>
 +
<li>Gel had no bands</li>
 +
<li>PCR amplified for infusion cloning</li>
 +
</ul>
 +
</div>
 +
 +
<div class = 'Days'><h5>7/15/16:</h5></div>
 +
<div class = 'Info'><ul><li>Ran Q1 and Q2 blocks in a 1.5% gel</li>
 +
<img src="https://static.igem.org/mediawiki/2016/d/d1/CSU_Q1Q271516.png">
 +
<li>Infusion cloning on Quorum and PSB1C3</li>
 +
<li>Transformed infusion mixture</li>
 +
</ul>
 +
</div>
 +
 +
<div class = 'Days'><h5>7/16/16:</h5></div>
 +
<div class = 'Info'><ul><li>PCRed PSB1C3 for infusion cloning</li>
 +
<li>Ran PCR products on a 1.5% gel</li>
 +
<img src="https://static.igem.org/mediawiki/2016/0/02/CSU_Psb1c71416.png">
 +
<li>Infusion performed on Quorum gBlocks and PSB1C3</li>
 +
<li>Transformed infusion product</li>
 +
</ul>
 +
</div>
 +
 +
<div class = 'Days'><h5>7/19/16:</h5></div>
 +
<div class = 'Info'><ul><li>2-step PCRed Qblocks together</li>
 +
<li>Ran PCR products on a 1.5% gel</li>
 +
<img src="https://static.igem.org/mediawiki/2016/2/28/CSU_Qblock71916.png">
 +
</ul>
 +
</div>
 +
 +
<div class = 'Days'><h5>7/20/16:</h5></div>
 +
<div class = 'Info'><ul><li>Redid Qblock 2-step PCR</li>
 +
<li>Ran PCR products on a 1.5% gel</li>
 +
<img src="https://static.igem.org/mediawiki/2016/7/7d/CSU_Q72016.png">
 +
</ul>
 +
</div>
 +
 +
<div class = 'Days'><h5>7/27/16:</h5></div>
 +
<div class = 'Info'><ul><li>Q1,Q2,PSB1C3 were all PCRed for ligase cycling</li>
 +
</ul>
 +
</div>
 +
 +
<div class = 'Days'><h5>7/27/16:</h5></div>
 +
<div class = 'Info'><ul> <li>Ran PCR products on a 1.5% gel</li>
 +
<img src="https://static.igem.org/mediawiki/2016/c/c4/CSU_PSQ1Q272716.png">
 +
</ul>
 +
</div>
 +
 +
<div class = 'Days'><h5>7/28/16:</h5></div>
 +
<div class = 'Info'><ul> <li>Digested Luc(ECORI XbaI) and Slr0118(ECORI XbaI)</li>
 +
<li>Ligated Slr0118 and Luc</li>
 +
<li> Transformed ligated mixture</li>
 +
</ul>
 +
</div>
 +
 +
<div class = 'Days'><h5>7/29/16:</h5></div>
 +
<div class = 'Info'><ul> <li>Dpn1 DIGESTED Q1 and Q2 PCR products</li>
 +
</ul>
 +
</div>
 +
 +
<div class = 'Days'><h5>7/30/16:</h5></div>
 +
<div class = 'Info'><ul> <li>Phosphorylated PSB1C3,Q1,and Q2 for ligase cycling</li>
 +
<li>Ligase cycling performed on phosphorylated mixture</li>
 +
<li>Transformed ligase cycling mixture</li>
 +
</ul>
 +
</div>
 +
 +
 +
<h2 class="left"><a href = "https://2016.igem.org/Team:CSU_Fort_Collins/NoteBook/June"> << June</a>
 +
 +
<h2 class="right"><a href = "https://2016.igem.org/Team:CSU_Fort_Collins/NoteBook/Aug">August >></a>
 +
 +
 +
</html>

Latest revision as of 16:43, 19 October 2016

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CSU iGEM



July

7/2/16:
  • Ran PCR products from inter lab study colony PCR on a 1% gel
  • No products
7/6/16:
  • PCRed out Lrta from Synechocystis PCC 6003
  • Re ran gel from inter lab study colony PCR on 1% gel
  • No products
7/7/16:
  • Ran Lrta PCR products on a 1% gel
7/8/16:
  • Performed ligase cycling reaction on slr0168 and pSB1C3
  • Ran all PCR products on 1%gel
  • Digested Lrta(SpeI EcorI)
  • Ligated Lrta and EYFP-LVA
  • Transformed Lrta+EYFP-LVA ligation mixture
7/12/16:
  • Dpn1 Digested slr0168 and pSB1C3
  • Phosphorylated slr0168 and pSB1C3
  • Performed ligase cycling reaction on phosphorylated mixture
  • Transformed ligase cycling reaction products
  • Digested EYFP-LVA(PstI)
  • Ligated EYFP-LVA + LrtA
  • Transformed EYFP-LVA+LrtA ligation mixture
  • Performed colony PCR on 13 colonies with the Q1 block
  • Ran PCR results on a 1.5% gel
7/13/16:
  • Colony PCR performed on 10 EYFP-LVA+LrtA transformed colonies
  • PCR amplified Q1 and Q2 for infusion cloning
7/14/16:
  • Ran Q1 and Q2 blocks in a 1.5% gel
  • Gel had no bands
  • PCR amplified for infusion cloning
7/15/16:
  • Ran Q1 and Q2 blocks in a 1.5% gel
  • Infusion cloning on Quorum and PSB1C3
  • Transformed infusion mixture
7/16/16:
  • PCRed PSB1C3 for infusion cloning
  • Ran PCR products on a 1.5% gel
  • Infusion performed on Quorum gBlocks and PSB1C3
  • Transformed infusion product
7/19/16:
  • 2-step PCRed Qblocks together
  • Ran PCR products on a 1.5% gel
7/20/16:
  • Redid Qblock 2-step PCR
  • Ran PCR products on a 1.5% gel
7/27/16:
  • Q1,Q2,PSB1C3 were all PCRed for ligase cycling
7/27/16:
  • Ran PCR products on a 1.5% gel
7/28/16:
  • Digested Luc(ECORI XbaI) and Slr0118(ECORI XbaI)
  • Ligated Slr0118 and Luc
  • Transformed ligated mixture
7/29/16:
  • Dpn1 DIGESTED Q1 and Q2 PCR products
7/30/16:
  • Phosphorylated PSB1C3,Q1,and Q2 for ligase cycling
  • Ligase cycling performed on phosphorylated mixture
  • Transformed ligase cycling mixture

<< June

August >>