Difference between revisions of "Team:CSU Fort Collins/NoteBook/Aug"

 
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<h1 align = "center">August</h1>
 
<h1 align = "center">August</h1>
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 +
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<div class = 'Days'><h5>8/5/16:</h5></div>
 +
<div class = 'Info'><ul><li>Phosphorylated Qblocks  and pSB1C3</li>
 +
<li>Performed ligase cycling on phosphorylated mixture, but instead used an annealing temperature of 55C</li>
 +
<li>Gel had no bands</li>
 +
</ul>
 +
</div>
  
  
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<li>Ligated I712013 and Luc</li>
 
<li>Ligated I712013 and Luc</li>
 
<li>Transformed ligation mixture </li>
 
<li>Transformed ligation mixture </li>
 +
</ul>
 +
</div>
 +
 +
<div class = 'Days'><h5>8/9/16:</h5></div>
 +
<div class = 'Info'><ul><li>PCRed out T7 polymerase N and C from T7 polymerase</li>
 +
</ul>
 +
</div>
 +
 +
<div class = 'Days'><h5>8/10/16:</h5></div>
 +
<div class = 'Info'><ul><li>Transformed new DNA samples for the inter lab study</li>
 
</ul>
 
</ul>
 
</div>
 
</div>
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<div class = 'Info'><ul><li>Colony PCRed transformed 21 I7+Luc colonies</li>
 
<div class = 'Info'><ul><li>Colony PCRed transformed 21 I7+Luc colonies</li>
 
<img src="https://static.igem.org/mediawiki/2016/d/da/CSU_LuCSlr0168Aug11.png">
 
<img src="https://static.igem.org/mediawiki/2016/d/da/CSU_LuCSlr0168Aug11.png">
 +
<li>Performed colony PCR on inter lab transformed colonies</li>
 +
<li>Ran results on a 1% gel </li>
 +
<li>No Products</li>
 
</ul>
 
</ul>
 
</div>
 
</div>
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<div class = 'Days'><h5>8/12/16:</h5></div>
 
<div class = 'Days'><h5>8/12/16:</h5></div>
 
<div class = 'Info'><ul><li>Phosphorylated Q1 and Q2 for ligase cycling</li>
 
<div class = 'Info'><ul><li>Phosphorylated Q1 and Q2 for ligase cycling</li>
 +
</ul>
 +
</div>
 +
 +
<div class = 'Days'><h5>8/14/16:</h5></div>
 +
<div class = 'Info'><ul><li>Redid colony PCR on inter lab study colonies</li>
 +
<li>Ran results on a 1% gel</li>
 +
<img src="https://static.igem.org/mediawiki/2016/c/c0/CSU_Interlab.png">
 
</ul>
 
</ul>
 
</div>
 
</div>
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</ul>
 
</ul>
 
</div>
 
</div>
 +
  
 
<div class = 'Days'><h5>8/23/16:</h5></div>
 
<div class = 'Days'><h5>8/23/16:</h5></div>
−
<div class = 'Info'><ul><li>Colony PCRed S</li>
+
<div class = 'Info'><ul><li>Performed colony PCR on Slr0168+Luc and Quorum  block</li>
−
<img src="https://static.igem.org/mediawiki/2016/7/73/CSU_CyanoAug19.jpg">
+
<img src="https://static.igem.org/mediawiki/2016/5/5f/CSU_HRQb82316.jpg">
 +
<li>Transformed Slr0168+Luc ligation mixture</li>
 +
<li>Performed 2-step PCR on Q1 and Q2</li>
 +
<li>RePCRed Q1,Q2,and pSB1C3 for ligase cycling</li>
 
</ul>
 
</ul>
 
</div>
 
</div>
  
 +
<div class = 'Days'><h5>8/25/16:</h5></div>
 +
<div class = 'Info'><ul><li>Digested Slr0168(ECORI PstI) and Luc(ECORI PstI)</li>
 +
<li>Ligated Slr0168 and Luc overnight at 16C</li>
 +
</ul>
 +
</div>
 +
 +
 +
<div class = 'Days'><h5>8/26/16:</h5></div>
 +
<div class = 'Info'><ul><li>Ran PCR products from the 23rd on a 1.5% gel</li>
 +
<img src="https://static.igem.org/mediawiki/2016/9/9e/CSU_Q1Q282616.png">
 +
 +
</ul>
 +
</div>
 +
 +
<div class = 'Days'><h5>8/30/16:</h5></div>
 +
<div class = 'Info'><ul><li>Slr0168+Luc and Qblock transformed colonies were colony PCRed with Q5 master mix</li>
 +
<img src="https://static.igem.org/mediawiki/2016/6/61/CSU_Luc%2BHrQb83016.jpg">
 +
<li>Slr0168+Luc colonies apear to have worked</li>
 +
 +
</ul>
 +
</div>
 +
 +
 +
<h2 class="left"><a href = "https://2016.igem.org/Team:CSU_Fort_Collins/NoteBook/July"> << July</a>
  
 +
<h2 class="right"><a href = "https://2016.igem.org/Team:CSU_Fort_Collins/NoteBook/Sep">September >></a>
  
 
</html>
 
</html>

Latest revision as of 16:57, 19 October 2016

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CSU iGEM



August

8/5/16:
  • Phosphorylated Qblocks and pSB1C3
  • Performed ligase cycling on phosphorylated mixture, but instead used an annealing temperature of 55C
  • Gel had no bands
8/6/16:
  • Performed Colony PCR on Slr0118+Luc transformed colonies
  • Ran PCR results on a 1% gel
  • Gel had no bands
8/8/16:
  • Digested I712013 and Luc
  • Ligated I712013 and Luc
  • Transformed ligation mixture
8/9/16:
  • PCRed out T7 polymerase N and C from T7 polymerase
8/10/16:
  • Transformed new DNA samples for the inter lab study
8/11/16:
  • Colony PCRed transformed 21 I7+Luc colonies
  • Performed colony PCR on inter lab transformed colonies
  • Ran results on a 1% gel
  • No Products
8/12/16:
  • Phosphorylated Q1 and Q2 for ligase cycling
8/14/16:
  • Redid colony PCR on inter lab study colonies
  • Ran results on a 1% gel
8/15/16:
  • Performed ligase cycling reaction on phosphorylated Q block mixture
8/19/16:
  • Performed colony PCR on Slr0168+Luc
8/23/16:
  • Performed colony PCR on Slr0168+Luc and Quorum block
  • Transformed Slr0168+Luc ligation mixture
  • Performed 2-step PCR on Q1 and Q2
  • RePCRed Q1,Q2,and pSB1C3 for ligase cycling
8/25/16:
  • Digested Slr0168(ECORI PstI) and Luc(ECORI PstI)
  • Ligated Slr0168 and Luc overnight at 16C
8/26/16:
  • Ran PCR products from the 23rd on a 1.5% gel
8/30/16:
  • Slr0168+Luc and Qblock transformed colonies were colony PCRed with Q5 master mix
  • Slr0168+Luc colonies apear to have worked

<< July

September >>