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So, the equation (1) can be modified to: | So, the equation (1) can be modified to: | ||
$$[suvCas9::sgRNA::PAMmer(nucleic)] / {c}_{0} = \frac{{K}_{0}}{{K}_{2}{c}_{R}(1 - t)\alpha + {K}_{2}{c}_{R}t + {K}_{0}}......(2)$$ | $$[suvCas9::sgRNA::PAMmer(nucleic)] / {c}_{0} = \frac{{K}_{0}}{{K}_{2}{c}_{R}(1 - t)\alpha + {K}_{2}{c}_{R}t + {K}_{0}}......(2)$$ | ||
− | The equation (2) is plotted below: | + | The equation (2) is plotted below:<br> |
− | <img src="https:// | + | <br> |
+ | <img src="https://static.igem.org/mediawiki/2016/d/d3/T--Tsinghua--model1.jpeg" style="width:50%;height:50%;margin-left:200px"> | ||
+ | <br> | ||
+ | <br> | ||
The relation between suvCas9::sgRNA::PAMmer(nucleic) and thymidine kinase(TK) expression is linear in the working condition: | The relation between suvCas9::sgRNA::PAMmer(nucleic) and thymidine kinase(TK) expression is linear in the working condition: | ||
$$[TK] = {k}_{0}[suvCas9::sgRNA::PAMmer(nucleic)]......(3)$$ | $$[TK] = {k}_{0}[suvCas9::sgRNA::PAMmer(nucleic)]......(3)$$ | ||
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${c}_{0}$: By changing the suvCas9 promotor and terminator. | ${c}_{0}$: By changing the suvCas9 promotor and terminator. | ||
${k}_{0}$: By changing the inducible promotor of the TK gene | ${k}_{0}$: By changing the inducible promotor of the TK gene | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
</div> | </div> | ||
Latest revision as of 23:43, 19 October 2016