Difference between revisions of "Team:Valencia UPV/Description"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the<a href="https://2016.igem.org/Judging/Medals"> improve a previous part or project gold medal criterion</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
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<h1>Project Description</h1>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<h5>Advice on writing your Project Description</h5>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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<h5>Inspiration</h5>
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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<section><div class="container-fluid"><div class="row"><div class="col-md-2 col-sm-3"><div class="side-nav margin-bottom-60 margin-top-30"><div class="side-nav-head"><button class="fa fa-bars"></button><h4>Index</h4></div><ul class="list-group list-group-bordered list-group-noicon uppercase"><li class="list-group-item"><a href="NOTAREALURL#HYPE-ITdescription_id"><span class="size-11 text-muted pull-right"></span>HYPE-IT description</a></li><li class="list-group-item"><a href="NOTAREALURL#HYPE-ITWorkflow_id"><span class="size-11 text-muted pull-right"></span>HYPE-IT Workflow</a></li></ul></div></div><div class="col-md-10 col-sm-9"><div class="blog-post-item" id="HYPE-ITdescription_id"><h3>HYPE-IT description</h3><p><br>HYPE-IT (Hack Your Plants Editing with Innovative Technologies) main goal is to develop an affordable technology for improving the accessibility of genome editing in plants with CRISPR/Cas9. Our strategy is based on a split Cas9 variant with viral vectors with the purpose to make gene knock-outs, leading in new enhanced plant varieties. A gene Database has been created, gathering identified referenced genes, in addition to more necessary information. There, we have related gene sequences with an specific phenotypic trait. Affordable laboratory equipment, such as thermocycler or centrifuge, as well as all the necessary biological parts, has been designed. All these tools and reagents have been gathered under a Lab-case. It has all the necessary things to make plant genome editing as an affordable, simple and accesible product, decreasing current technological barriers for plant breeders.<br><br>One of the key elements in our project is the split-Cas9 system, designed to allow the delivery of Cas9 in plants with efficient viral vectors. To allow the reconstitution of the divided Cas9, he have used the inteins of 2014 Heidelberg Team (BBa_K1362400 and BBa_K1362401. We have improved its characterization by demonstrating its functionality in plants (read more about Split-Cas9).<br><br><br></p></div><div class="blog-post-item" id="HYPE-ITWorkflow_id"><h3>HYPE-IT Workflow</h3><p>We have designed a Workflow, where we illustrate how a plant breeder could obtain his desired improve variety by HYPE-IT system:<br></p><ol><li>The plant breeder access to HYPE-IT Database to choose the genes to knock-out. The only thing he has to decide is what phenotypic trait he wants to obtain, as well as the species that he is going to work with. </li><li>HYPE-IT Software give him predicted gRNAs for the targeted gene. </li><li>These gRNAs are ordered by a scoring system, from the optimal one to the less accurate. Then, he order the gRNA synthesis. </li><li>If he wants to be sure the gRNA is properly designed, a luciferase based gRNA testing system has been designed to check if this gRNA will work before its integration in the targeted plant. </li><li>After that, split Cas9 infiltration is delivered by viral system. </li><li>He waits until the plant grows enough. </li><li>Then, he regenerates new plants from modified plants. </li><li>These plants are regenerated from modified cells, so the whole plant is edited.</li><li>The plant breeder harvest modified fruits. </li></ol><p><br></p><div style="text-align:center;"><img class="img-responsive" style="width:600px" src="https://static.igem.org/mediawiki/2016/3/37/T--Valencia_UPV--HYPE-IT-workflow.jpg"></div><p><br></p></div></div></div></section>
  
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Latest revision as of 03:01, 20 October 2016

Project Description

Index

HYPE-IT description


HYPE-IT (Hack Your Plants Editing with Innovative Technologies) main goal is to develop an affordable technology for improving the accessibility of genome editing in plants with CRISPR/Cas9. Our strategy is based on a split Cas9 variant with viral vectors with the purpose to make gene knock-outs, leading in new enhanced plant varieties. A gene Database has been created, gathering identified referenced genes, in addition to more necessary information. There, we have related gene sequences with an specific phenotypic trait. Affordable laboratory equipment, such as thermocycler or centrifuge, as well as all the necessary biological parts, has been designed. All these tools and reagents have been gathered under a Lab-case. It has all the necessary things to make plant genome editing as an affordable, simple and accesible product, decreasing current technological barriers for plant breeders.

One of the key elements in our project is the split-Cas9 system, designed to allow the delivery of Cas9 in plants with efficient viral vectors. To allow the reconstitution of the divided Cas9, he have used the inteins of 2014 Heidelberg Team (BBa_K1362400 and BBa_K1362401. We have improved its characterization by demonstrating its functionality in plants (read more about Split-Cas9).


HYPE-IT Workflow

We have designed a Workflow, where we illustrate how a plant breeder could obtain his desired improve variety by HYPE-IT system:

  1. The plant breeder access to HYPE-IT Database to choose the genes to knock-out. The only thing he has to decide is what phenotypic trait he wants to obtain, as well as the species that he is going to work with.
  2. HYPE-IT Software give him predicted gRNAs for the targeted gene.
  3. These gRNAs are ordered by a scoring system, from the optimal one to the less accurate. Then, he order the gRNA synthesis.
  4. If he wants to be sure the gRNA is properly designed, a luciferase based gRNA testing system has been designed to check if this gRNA will work before its integration in the targeted plant.
  5. After that, split Cas9 infiltration is delivered by viral system.
  6. He waits until the plant grows enough.
  7. Then, he regenerates new plants from modified plants.
  8. These plants are regenerated from modified cells, so the whole plant is edited.
  9. The plant breeder harvest modified fruits.



Sponsors