Difference between revisions of "Team:WashU StLouis/Notebook"

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<div class = "full_size">
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<div class = "column full_size">
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 +
<div class = "title2"></div>
 +
<!--<div class="action"><img  src="https://static.igem.org/mediawiki/2016/d/d9/T--WashU_StLouis--Action.png" style="width:100%;"></div>-->
 +
<div class = "speech"><img  src="https://static.igem.org/mediawiki/2016/0/05/T--WashU_StLouis--Speech.png" style="width:100%;"></div>
 +
 
 +
<h1 class="actionwords">Notebook</h1>
 +
<h2 class="speechwords">Reading about labwork is <i> almost </i> as fun as doing labwork</h2>
 +
 
 +
 
 
<div class = "calendar-wrapper">
 
<div class = "calendar-wrapper">
 
<div class="month-calendar june" style = "display:block;">
 
<div class="month-calendar june" style = "display:block;">
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<div class="slideshow-container">
 
<div class="slideshow-container">
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
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    <div class="numbertext">1 / 30</div>
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     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 1 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">June 1st</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
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    <div class="numbertext">2 / 30</div>
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     <div style="height: 200px; width: 100%;">
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     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 2 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">June 2nd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">3 / 30</div>
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     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 3 </p>
+
<ul>
 +
<li>First day in lab!</li>
 +
<li>Made 1L of LB Media</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 3rd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">4 / 30</div>
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     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 4 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">June 4th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">5 / 30</div>
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     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 5 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">June 5th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">6 / 30</div>
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     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Extracted Genomic DNA from MG1655 and  6803 (See Protocol)</p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">June 6th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">7 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 7 </p>
+
<ul>
 +
<li>Performed gDNA Extraction on E. Coli MG1655 and Synechocystis 6803</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 7th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">8 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 8 </p>
+
<ul>
 +
<li>Designed PCR primers for <i>pgk</i>, <i>pck</i>, <i>fldA</i>, <i>petF</i>, ATP Synthase 1, and ATP Synthase 2</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 8th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">9 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 9 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">June 9th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">10 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Ran PCR for petF gene, fldA gene, and pdCas9 plasmid </p>
+
<ul>
−
    <p>Made gel and ran PCR product</p>
+
<li>Primers Arrived</li>
−
    <p>Extracted DNA from gel</p>
+
<li>Ran PCR on ATP Synthase 2 , <i><i>pgk</i></i>, <i><i>fldA</i></i>, <i><i>petF</i></i>, and pdCas9 plasmid</li>
−
    <p>Re-ran pdCas9 PCR</p>
+
<li>Ran all PCR products on gel and recovered ATP Synthase 2, <i><i>fldA</i></i>, and <i><i>petF</i></i></li>
 +
<li>Ran gradient PCR on pdCas9 plasmid</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 10th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">11 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 11 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">June 11th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">12 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 12 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">June 12th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">13 / 30</div>
+
 
−
     <div style="height: 200px; width: 400px;">
+
     <div style="height: 200px; width: 100%;">
 +
 
 
     </br></br>
 
     </br></br>
−
    <p> Made and ran gel for pdCas9</p>
+
<ul>
−
<p>Extracted DNA</p>
+
<li> Made and ran gel for pdCas9</li>
−
<p>Made agar plates</p>
+
<li>Ran PCR on ATP Synthase 1, <i><i>pgk</i></i>, and  <i><i>pck</i></i></li>
−
<p>Ran golden gate one pot assembly for both genes</p>
+
<li>Performed gel extraction on yesterday’s ATP Synthase 2</li>
 +
<li>Ran  <i><i>pck</i></i>, <i><i>pgk</i></i>, and yesterday’s gradient pdCas9 PCR products on gel and recovered all genes</li>
 +
<li>Performed gel extraction on pdCas9, <i>fldA</i>, <i>petF</i></li>
 +
<li>Ran PCR on psc101 and psc102 plasmid</li>
 +
<li>Ran psc101 and psc102 PCR products on gel - were recovered</li>
 +
<li>Ran gradient PCR on ATP Synthase 1</li>
 +
<li>Made agar plates (forgot the antibiotic)</li>
 +
<li>Ran golden gate one pot assembly for <i>fldA</i> <i>petF</i> genes and the pdCas9 plasmid</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 13th</div>
+
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">14 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 14 </p>
+
<ul>
 +
<li>Ran ATP Synthase 1 PCR products on gel - no bands seen</li>
 +
<li>Gel Extraction for <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid</li>
 +
<li>Ran PCR on ATP Synthase 1 (3rd times the charm)</li>
 +
<li>Ran PCR products on gel - WE SAW BANDS! ATP SYNTHASE 1 RECOVERED</li>
 +
<li>Reran PCR on <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid because we did not get high enough concentration during gel Extraction</li>
 +
<li>Made new agar plates (with the chloramphenicol)</li>
 +
<li>Transformed the <i>petF</i>, <i>fldA</i> plasmids into competent cells</li>
 +
<li>Plated colonies on agar plates and set in incubator</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 14th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">15 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 15 </p>
+
<ul>
 +
<li>Checked for colonies (both genes were successful)</li>
 +
<li>Picked colonies and incubated overnight</li>
 +
<li>Ran yesterday’s PCR products on gel - bands were light but all were recovered</li>
 +
<li>Gel Extraction for <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid</li>
 +
<li>Reran PCR on <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid because we did not get high enough concentration during gel extraction</li>
 +
<li>Ran PCR products on gel - all were recovered</li>
 +
<li>Gel Extraction for <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 15th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">16 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 16 </p>
+
<ul>
 +
<li>Miniprepped plasmids for <i>fldA</i>, <i>petF</i></li>
 +
<li>Ran sequence PCR on both plasmids</li>
 +
<li>Ran PCR product on gel to confirm</li>
 +
<li>Clean and Concentrated PCR product</li>
 +
<li>Reran PCR on <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid because we did not get high enough concentration during gel extraction (for the fourth time)</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 16th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">17 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 17 </p>
+
<ul>
 +
<li>Prepared DNA for sequencing</li>
 +
<li>Delivered DNA to WashU Med School</li>
 +
<li>Added DPnI to psc101 and psc102 gel extract products, incubated for an hour, did Clean and Concentrate, measured conc</li>
 +
<li>Ran Golden Gate assembly on <i>pck</i>, <i>pgk</i>, and ATP Synthase</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 17th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">18 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 18 </p>
+
<ul>
 +
<li>Electroporation with golden gate products</li>
 +
<li>Redid golden gate for <i>pck</i>, <i>pgk</i>, ATP synthase (did not run properly first time)</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 18th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">19 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 19 </p>
+
<ul>
 +
<li>Electroporation with better golden gate products</li>
 +
<li>Picked colonies for worse golden gate for <i>pck</i>, <i>pgk</i>, ATP synthase</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 19th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">20 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 20 </p>
+
<ul>
 +
<li>Miniprep worse ATP, <i>pck</i>, <i>pgk</i> golden gate</li>
 +
<li>Seq PCR worse ATP, <i>pck</i>, <i>pgk</i> golden gate</li>
 +
<li>Picked colonies for better golden gate for <i>pck</i>, <i>pgk</i>, ATP synthase</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 20th</div>
 
 
   </div>
 
   </div>
  
 
<div class="mySlides fade">
 
<div class="mySlides fade">
−
    <div class="numbertext">21 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 21 </p>
+
<ul>
 +
<li>Made frozen stock of better golden gate <i>pck</i>, <i>pgk</i>, ATP synthase</li>
 +
<li>Miniprepped better golden gate <i>pck</i>, <i>pgk</i>, ATP synthase (low concentration)</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 21st</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">22 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 22 </p>
+
<ul>
−
    </div>
+
<li>Ran sequencing PCR for <i>pgk</i>, <i>pck</i> (4 colonies each)</li>
−
    <div class="text">June 22nd</div>
+
<li>Gel Extraction for <i>pgk</i>, <i>pck</i> PCR product (no bands)</li>
 +
</ul>   </div>
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">23 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 23 </p>
+
<ul>
 +
<li>Ran sequencing PCR for <i>pck</i> and <i>pgk</i> on a gradient</li>
 +
<li>Gel Extraction for <i>pck</i> PCR product was successful, not <i>pgk</i></li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 23rd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">24 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 24 </p>
+
<ul>
 +
<li>Ran sequencing PCR for <i>pgk</i> on a different gradient</li>
 +
<li>Gel Extraction for <i>pgk</i> PCR product was not successful</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 24th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">25 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 25 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">June 25th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">26 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 26 </p>
+
<ul>
 +
<li>Picked four new colonies of <i>pgk</i></li>
 +
<li>Started 4 new cultures from frozen stock</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 26th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">27 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 27 </p>
+
<ul>
 +
<li>Made frozen stock of the 4 new colonies</li>
 +
<li>Miniprepped all 8 <i>pgk</i> cultures and measured concentrations</li>
 +
<li>Ran gradient PCR on all 8 <i>pgk</i> plasmids</li>
 +
<li>Prepped and sent ATP synthase and <i>pck</i> in for sequencing</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 27th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">28 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 28 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">June 28th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">29 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 29 </p>
+
<ul>
 +
<li>Mixed new <i>pgk</i> sequencing primers</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 29th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">30 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 30 </p>
+
<ul>
 +
<li>Ran gradient PCR on all 8 <i>pgk</i> plasmids</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">June 30th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">1 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 31 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">July 1st</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">2 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 32 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">July 2nd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">3 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 33 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">July 3rd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">4 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 34 </p>
+
     <p> 4th of July! </p>
 
     </div>
 
     </div>
−
    <div class="text">July 4th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">5 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 35 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">July 5th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">6 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 36 </p>
+
<ul>
 +
<li>Sequencing results for ATP and <i>pck</i> were not good</li>
 +
<li>Reran seq PCR on ATP Synthase (bad results)</li>
 +
<li>Picked new ATP colonies</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 6th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">7 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 37 </p>
+
<ul>
 +
<li>Miniprepped overnight cultures of ATP 5-8 and forgot to make frozen stock</li>
 +
<li>Ran PCR on ATP plasmids 5-8</li>
 +
<li>PCR for <i>pgk</i> with new primers with gradient</li>
 +
<li>Ran <i>pgk</i> PCR product on gels</li>
 +
<li>Ran golden gate on <i>pgk</i> PCR gel extract</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 7th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">8 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 38 </p>
+
<ul>
 +
<li>Electroporation with golden gate <i>pgk</i> product</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 8th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">9 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 39 </p>
+
<ul>
 +
<li>Picked 4 <i>pgk</i> products and inoculated them overnight</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 9th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">10 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 40 </p>
+
<ul>
 +
<li>Made frozen stock of new 4 <i>pgk</i> cultures</li>
 +
<li>Miniprepped cultures</li>
 +
<li>Seq PCR on new cultures</li>
 +
<li>All 4 cultures showed correct bands</li>
 +
<li>Clean and Concentrate <i>pgk</i> PCR product</li>
 +
<li>Prepped <i>pgk</i> for sequencing</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 10th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">11 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 41 </p>
+
<ul>
 +
<li>Delivered <i>pgk</i> for sequencing</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 11th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">12 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 42 </p>
+
<ul>
−
    </div>
+
<li>New ATP synthase sequencing primers and <i>pck</i> regular primers came in</li>
−
    <div class="text">July 12th</div>
+
<li>Seq PCR of ATP synthase plasmids with new sequencing primers. No bands</li>
 +
<li>Picked 4 new ATP synthase colonies (again)</li>
 +
<li>Inoculated MG1655 from frozens stock to make more genomic DNA</li>
 +
</ul>   </div>
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">13 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 43 </p>
+
<ul>
 +
<li>Extracted genomic DNA from MG1655</li>
 +
<li>Miniprepped new ATP cultures</li>
 +
<li>Ran sequencing PCR on new ATP and got no bands at all. Will use newly transformed cells tomorrow</li>
 +
<li>Ran PCR with <i>pck</i> primers, got light bands</li>
 +
<li>Reran PCR with <i>pck</i> products</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 13th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">14 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 44 </p>
+
<ul>
 +
<li>Ran <i>pck</i> on gel</li>
 +
<li>Gel extracted <i>pck</i> and combined gel extracts to improve concentration</li>
 +
<li>Golden gate ligation on <i>pck</i></li>
 +
<li>Ran ATP sequencing PCR again with different backbone primers</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 14th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">15 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 45 </p>
+
<ul>
 +
<li>Ran gel from yesterday’s ATP PCR. No bands</li>
 +
<li>Ran PCR for <i>pfo</i></li>
 +
<li>Incubated <i>pck</i> and BioBrick cells</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 15th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">16 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 46 </p>
+
<ul>
 +
<li>Miniprepped <i>pck</i> and biobrock genes (HSP)</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 16th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">17 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 47 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">July 17th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">18 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 48 </p>
+
<ul>
−
    </div>
+
<li>Ran ATP seq PCR gradient again. No bands. Done with ATP</li>
−
    <div class="text">July 18th</div>
+
<li>BioBrick digestion, ligation, transformation with HSP and GFP</li>
 +
<li>Ran PCR seq for <i>pck</i> and gel extract</li>
 +
</ul>
 +
  </div>
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">19 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 49 </p>
+
<ul>
 +
<li>Delivered <i>pck</i> sequence to med school</li>
 +
<li>Ligated backbone for BioBrick</li>
 +
<li>Extracted more genomic DNA from MG1655</li>
 +
<li>Gradient PCR on <i>pfo</i></li>
 +
<li>Received 𝛥<i>aceE</i> cells from Yale</li>
 +
<li>Rehydrated and incubated 𝛥<i>aceE</i> cells</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 19th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">20 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 50 </p>
+
<ul>
 +
<li>Ran PCR and gel extract for <i>fldA</i> backbone and <i>petF</i> backbone</li>
 +
<li>DPn1 the two backbones</li>
 +
<li>golden gate ligation on <i>pfo</i> gene and two backbones</li>
 +
<li>Streaked colony from 𝛥<i>aceE</i> plate onto LB/kan plate</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 20th</div>
 
 
   </div>
 
   </div>
  
 
<div class="mySlides fade">
 
<div class="mySlides fade">
−
    <div class="numbertext">21 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 51 </p>
+
<ul>
 +
<li>Making media for competent cell protocol</li>
 +
<li>Made new LB with MgSO4 for chemical transformation</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 21st</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">22 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 52 </p>
+
<ul>
 +
<li>Digested BioBrick miniprep to check length</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 22nd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">23 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 53 </p>
+
<ul>
 +
<li>Chemically competent cell protocol with 𝛥<i>aceE</i></li>
 +
<li>Miniprepped <i>pfo</i>-<i>petF</i> and <i>fldA</i>-<i>petF</i></li>
 +
<li>Seq PCR miniprepped <i>pfo</i>-<i>petF</i> and <i>fldA</i>=<i>petF</i></li>
 +
<li>Remade LB with MgSO4 with correct concentration</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 23rd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">24 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 54 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">July 24th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">25 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 55 </p>
+
<ul>
 +
<li>Restarted comp cell protocol (3rd times the charm)</li>
 +
<li>Clean and Concentrate junction 2 for <i>pfo</i>-<i>fldA</i></li>
 +
<li>Conducted HSP test in LB</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 25th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">26 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 56 </p>
+
<ul>
 +
<li>Redid HSP test in minimal media M9</li>
 +
<li>Recultured BioBrick and DH10B control</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 26th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">27 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 57 </p>
+
<ul>
 +
<li>Reset up BioBrick experiment but grew diluted cells with LB instead of M9, so we have to do it again</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 27th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">28 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 58 </p>
+
<ul>
 +
<li>Redid HSP test in minimal media M9</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">July 28th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">29 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 59 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">July 29th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">30 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 60 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">July 30th</div>
 
 
   </div>
 
   </div>
 
    
 
    
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">31 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 61 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">July 31st</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">1 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 62 </p>
+
<ul>
 +
<li>Chemical transformation of <i>pfo</i>-<i>petF</i>, <i>pfo</i>-<i>fldA</i>, <i>petF</i>, <i>fldA</i> into knockout strain, plated 80 and 240 ul, also control</li>
 +
<li>Part 1 of interlab study (LUDOX)</li>
 +
<li>Transforming 5 interlab plasmids</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 1st</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">2 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 63 </p>
+
<ul>
 +
<li>Picked and incubated electron donor colonies plated yesterday</li>
 +
<li>Picked and incubated interlab colonies plated yesterday (2 per construct)</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 2nd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">3 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 64 </p>
+
<p><ul>
 +
<li>Made frozen stock of incubated colonies and interlab parts</li>
 +
<li>Redid BioBrick experiment at 37C</li>
 +
</ul></p>
 
     </div>
 
     </div>
−
    <div class="text">August 3rd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">4 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 65 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">August 4th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">5 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 66 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">August 5th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">6 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 67 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">August 6th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">7 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 68 </p>
+
<ul>
 +
<li>Started induction but had to stop bc no frozen stock of <i>fldA</i>-<i>pfo</i> or <i>petF</i>-<i>fldA</i> in DH10B</li>
 +
<li>Transformed <i>fldA</i>-<i>pfo</i> and <i>petF</i>-<i>pfo</i> into DH10B</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 7th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">8 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 69 </p>
+
<ul>
 +
<li>Made frozen stock of 𝛥<i>aceE</i></li>
 +
<li>Made 60% ethanol</li>
 +
<li>Incubated 8 constructs for biotin assay w/ methanol extraction (4 constructs and blank in DH10B and 𝛥<i>aceE</i>)</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 8th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">9 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 70 </p>
+
<ul>
 +
<li>Methanol extraction assay (grew cells and froze pellets)</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 9th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">10 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 71 </p>
+
<ul>
 +
<li>Assayed <i>petF</i>-<i>aceE</i> cells. Absorbance was too low to be meaningful</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 10th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">11 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 72 </p>
+
<ul>
 +
<li>Ran preliminary sonication test</li>
 +
<li>Incubated large volume of DH10B</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 11th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">12 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 73 </p>
+
<ul>
 +
<li>Ran large sonication test on DH10B to decide protocol</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 12th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">13 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 74 </p>
+
<ul>
 +
<li>Incubated large volume of DH10B</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 13th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">14 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 75 </p>
+
<ul>
 +
<li>More sonication tests</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 14th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">15 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 76 </p>
+
<ul>
 +
<li>Made ATP standards from assay instructions</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 15th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">16 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 77 </p>
+
<ul>
 +
<li>Made more LB</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 16th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">17 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 78 </p>
+
<ul>
 +
<li>Inoculated <i>pgk</i>, <i>pck</i>, DH10B for assay tomorrow</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 17th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">18 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 79 </p>
+
<ul>
−
    </div>
+
<li>ATP luminescence assay</li>
−
    <div class="text">August 18th</div>
+
<li>Inoculated 4 constructs and blank in 𝛥<i>aceE</i> cells for tomorrow</li>
 +
</ul>   </div>
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">19 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 80 </p>
+
<ul>
 +
<li>Nothing grew, couldn’t do induction</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 19th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">20 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 81 </p>
+
<ul>
 +
<li>Inoculated DH10B constructs and control from frozen stock</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 20th</div>
 
 
   </div>
 
   </div>
  
 
<div class="mySlides fade">
 
<div class="mySlides fade">
−
    <div class="numbertext">21 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 82 </p>
+
<ul>
 +
<li>DH10B electron donor induction, froze pellets, measured OD600</li>
 +
<li>Re-transformed constructs into 𝛥<i>aceE</i> cells</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 21st</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">22 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 83 </p>
+
<ul>
 +
<li>Sonicated and assayed pellets for biotin (1 minute, 40 amp, 1mL PBS)</li>
 +
<li>Reinoculated 𝛥<i>aceE</i> constructs in all antibiotic combinations to determine problem, in LB and plates</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 22nd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">23 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 84 </p>
+
<ul>
 +
<li>Visited Monsanto</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 23rd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">24 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 85 </p>
+
<ul>
 +
<li>Measured absorbance data from biotin in DH10B cells</li>
 +
<li>Did ATP Assay and got luminescence data</li>
 +
<li>Incubated all four 𝛥<i>aceE</i> electron donor constructs from frozen stock plates and original plates and incubated 𝛥<i>aceE</i> and DH10B</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 24th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">25 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 86 </p>
+
<ul>
 +
<li>Nothing grew from yesterday's inoculations</li>
 +
<li>Interlab study</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 25th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">26 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 87 </p>
+
<ul>
 +
<li>Remade competent cells</li>
 +
<li>Finished interlab study (FITC) and sent to iGEM HQ</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">August 26th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">27 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 88 </p>
+
     <p> No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">August 27th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">28 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 89 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">August 28th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">29 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 90 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">August 29th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">30 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 91 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">August 30th</div>
 
 
   </div>
 
   </div>
 
    
 
    
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">31 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 92 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">August 31st</div>
 
 
   </div>
 
   </div>
  
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">1 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 93 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 1st</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">2 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 94 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 2nd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">3 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 95 </p>
+
<ul>
 +
<li>Picked colonies from 4 new 𝛥<i>aceE</i> plates and incubated</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">September 3rd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">4 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 96 </p>
+
<ul>
 +
<li>𝛥<i>aceE</i> cell induction</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">September 4th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">5 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 97 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 5th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">6 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 98 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 6th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">7 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 99 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 7th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">8 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 100 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 8th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">9 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 101 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 9th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">10 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 102 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 10th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">11 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 103 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 11th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">12 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 104 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 12th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">13 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 105 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 13th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">14 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 106 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 14th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">15 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 107 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 15th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">16 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 108 </p>
+
<ul>
 +
<li>Incubated <i>pck</i>, <i>pgk</i>, DH10B from frozen stock</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">September 16th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">17 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 109 </p>
+
<ul>
 +
<li>Ran ATP assay</li>
 +
<li>BioBrick digestion and ligation</li>
 +
<li>Sonicated 𝛥<i>aceE</i> pellets</li>
 +
<li>Ran 𝛥<i>aceE</i> cell absorbance scan</li>
 +
<li>Ran biotin assay on 𝛥<i>aceE</i> cells</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">September 17th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">18 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 110 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 18th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">19 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 111 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 19th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">20 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 112 </p>
+
     <p> No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 20th</div>
 
 
   </div>
 
   </div>
  
 
<div class="mySlides fade">
 
<div class="mySlides fade">
−
    <div class="numbertext">21 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 113 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 21st</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">22 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 114 </p>
+
     <p> No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 22nd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">23 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 115 </p>
+
     <p> No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 23rd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">24 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 116 </p>
+
     <p> No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 24th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">25 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 117 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 25th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">26 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 118 </p>
+
<ul>
 +
<li>Transformed ligated <i>petF</i> and <i>pck</i> BioBrick plasmids into DH10B (wrong antibiotic)</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">September 26th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">27 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 119 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">September 27th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">28 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 120 </p>
+
<ul>
 +
<li>Transformed ligated <i>petF</i> and <i>pck</i> BioBrick plasmids into DH10B (kan = right)</li>
 +
<li>Wrapped <i>pck</i> and <i>pgk</i> plasmids to be sent to Cardiff University iGEM</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">September 28th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">29 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 121 </p>
+
<ul>
 +
<li>Small colonies grew on all plates</li>
 +
<li>Picked colonies and inoculated</li>
 +
<li>Sent plasmids to Cardiff University iGEM</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">September 29th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">30 / 30</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 122 </p>
+
<ul>
 +
<li>Miniprep on <i>pck</i> and <i>petF</i> BioBrick</li>
 +
<li>Reusupended TetR-pTet gBlocks</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">September 30th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">1 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 93 </p>
+
<ul>
 +
<li>Digestion/Ligation/Transformation of pSB1C3 and tetr-ptet BioBrick</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">October 1st</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">2 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 94 </p>
+
<ul>
 +
<li>TetR-pTet colonies grew, picked colonies</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">October 2nd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">3 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 95 </p>
+
<ul>
 +
<li>Miniprepped TetR-pTet BioBrick</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">October 3rd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">4 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 96 </p>
+
<ul>
 +
<li>digested BioBricks to check bands sizes</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">October 4th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">5 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 97 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">October 5th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">6 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 98 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">October 6th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">7 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 99 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">October 7th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">8 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 100 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">October 8th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">9 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 101 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">October 9th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">10 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 102 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">October 10th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">11 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 103 </p>
+
     <p>No lab work done</p>
 
     </div>
 
     </div>
−
    <div class="text">October 11th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">12 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 104 </p>
+
<ul>
 +
<li>Ran PCR on BioBrick plasmids</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">October 12th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">13 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 105 </p>
+
<ul>
 +
<li>Ran PCR products on gel</li>
 +
<li>Only <i>petF</i> showed up</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">October 13th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">14 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 106 </p>
+
<ul>
 +
<li>digestion of <i>pck</i>, TetR-pTet, and pSB1C3 with EcoRI</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">October 14th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">15 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 107 </p>
+
<ul>
 +
<li>Digestion of <i>pck</i>, TetR-pTet, and pSB1C3 with PstI</li>
 +
<li>Ligated <i>pck</i> and TetR-pTet to pSB1C3</li>
 +
<li>Transformed into DH10B</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">October 15th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">16 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 108 </p>
+
<ul>
 +
<li>Only TetR-pTet grew</li>
 +
<li>Ran colony PCR with 8 <i>pck</i> colonies and 8 TetR-pTet colonies</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">October 16th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">17 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 109 </p>
+
<ul>
 +
<li>Ran colony PCR products on a gel but got no bands</li>
 +
<li>Miniprepped pTet1-8</li>
 +
<li>Ran PCR on pTet 1-8 plasmids to check if insert was present</li>
 +
<li>Ran PCR product on gel, got the band!</li>
 +
<li>Prepped <i>petF</i>, <i>pck</i>, and TetR-pTet to be sent to iGEM HQ</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">October 17th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">18 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
    <p> Stuff that happened on day 110 </p>
+
<ul>
 +
<li>Last day in lab!</li>
 +
<li>Sent parts to iGEM!</li>
 +
</ul>
 
     </div>
 
     </div>
−
    <div class="text">October 18th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">19 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 111 </p>
+
     <p>WIKIFREEZE</p>
 
     </div>
 
     </div>
−
    <div class="text">October 19th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">20 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 112 </p>
+
     <p>Done in lab</p>
 
     </div>
 
     </div>
−
    <div class="text">October 20th</div>
 
 
   </div>
 
   </div>
  
 
<div class="mySlides fade">
 
<div class="mySlides fade">
−
    <div class="numbertext">21 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 113 </p>
+
     <p> Prepping for Jamboree </p>
 
     </div>
 
     </div>
−
    <div class="text">October 21st</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">22 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 114 </p>
+
     <p> Prepping for Jamboree </p>
 
     </div>
 
     </div>
−
    <div class="text">October 22nd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">23 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 115 </p>
+
     <p>Prepping for Jamboree</p>
 
     </div>
 
     </div>
−
    <div class="text">October 23rd</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">24 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 116 </p>
+
     <p> Prepping for Jamboree </p>
 
     </div>
 
     </div>
−
    <div class="text">October 24th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">25 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 117 </p>
+
     <p> Prepping for Jamboree </p>
 
     </div>
 
     </div>
−
    <div class="text">October 25th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">26 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 118 </p>
+
     <p> Prepping for Jamboree </p>
 
     </div>
 
     </div>
−
    <div class="text">October 26th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">27 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 119 </p>
+
     <p>Fly to Boston for Jamboree</p>
 
     </div>
 
     </div>
−
    <div class="text">October 27th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">28 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 120 </p>
+
     <p> GIANT JAMBOREE</p>
 
     </div>
 
     </div>
−
    <div class="text">October 28th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">29 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 121 </p>
+
     <p>GIANT JAMBOREE</p>
 
     </div>
 
     </div>
−
    <div class="text">October 29th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">30 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 122 </p>
+
     <p>PRESENTATION</p>
 
     </div>
 
     </div>
−
    <div class="text">October 30th</div>
 
 
   </div>
 
   </div>
  
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
−
    <div class="numbertext">31 / 31</div>
+
     <div style="height: 200px; width: 100%;">
−
     <div style="height: 200px; width: 400px;">
+
 
     </br></br>
 
     </br></br>
−
     <p> Stuff that happened on day 122 </p>
+
     <p> Fly back to St. Louis</p>
 
     </div>
 
     </div>
−
    <div class="text">October 31st</div>
 
 
   </div>
 
   </div>
  

Latest revision as of 03:10, 20 October 2016

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Notebook

Reading about labwork is almost as fun as doing labwork

June
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30


No lab work done



No lab work done



  • First day in lab!
  • Made 1L of LB Media


No lab work done



No lab work done



No lab work done



  • Performed gDNA Extraction on E. Coli MG1655 and Synechocystis 6803


  • Designed PCR primers for pgk, pck, fldA, petF, ATP Synthase 1, and ATP Synthase 2


No lab work done



  • Primers Arrived
  • Ran PCR on ATP Synthase 2 , pgk, fldA, petF, and pdCas9 plasmid
  • Ran all PCR products on gel and recovered ATP Synthase 2, fldA, and petF
  • Ran gradient PCR on pdCas9 plasmid


No lab work done



No lab work done



  • Made and ran gel for pdCas9
  • Ran PCR on ATP Synthase 1, pgk, and pck
  • Performed gel extraction on yesterday’s ATP Synthase 2
  • Ran pck, pgk, and yesterday’s gradient pdCas9 PCR products on gel and recovered all genes
  • Performed gel extraction on pdCas9, fldA, petF
  • Ran PCR on psc101 and psc102 plasmid
  • Ran psc101 and psc102 PCR products on gel - were recovered
  • Ran gradient PCR on ATP Synthase 1
  • Made agar plates (forgot the antibiotic)
  • Ran golden gate one pot assembly for fldA petF genes and the pdCas9 plasmid


  • Ran ATP Synthase 1 PCR products on gel - no bands seen
  • Gel Extraction for pgk, pck, psc101, and psc102 plasmid
  • Ran PCR on ATP Synthase 1 (3rd times the charm)
  • Ran PCR products on gel - WE SAW BANDS! ATP SYNTHASE 1 RECOVERED
  • Reran PCR on pgk, pck, psc101, and psc102 plasmid because we did not get high enough concentration during gel Extraction
  • Made new agar plates (with the chloramphenicol)
  • Transformed the petF, fldA plasmids into competent cells
  • Plated colonies on agar plates and set in incubator


  • Checked for colonies (both genes were successful)
  • Picked colonies and incubated overnight
  • Ran yesterday’s PCR products on gel - bands were light but all were recovered
  • Gel Extraction for pgk, pck, psc101, and psc102 plasmid
  • Reran PCR on pgk, pck, psc101, and psc102 plasmid because we did not get high enough concentration during gel extraction
  • Ran PCR products on gel - all were recovered
  • Gel Extraction for pgk, pck, psc101, and psc102 plasmid


  • Miniprepped plasmids for fldA, petF
  • Ran sequence PCR on both plasmids
  • Ran PCR product on gel to confirm
  • Clean and Concentrated PCR product
  • Reran PCR on pgk, pck, psc101, and psc102 plasmid because we did not get high enough concentration during gel extraction (for the fourth time)


  • Prepared DNA for sequencing
  • Delivered DNA to WashU Med School
  • Added DPnI to psc101 and psc102 gel extract products, incubated for an hour, did Clean and Concentrate, measured conc
  • Ran Golden Gate assembly on pck, pgk, and ATP Synthase


  • Electroporation with golden gate products
  • Redid golden gate for pck, pgk, ATP synthase (did not run properly first time)


  • Electroporation with better golden gate products
  • Picked colonies for worse golden gate for pck, pgk, ATP synthase


  • Miniprep worse ATP, pck, pgk golden gate
  • Seq PCR worse ATP, pck, pgk golden gate
  • Picked colonies for better golden gate for pck, pgk, ATP synthase


  • Made frozen stock of better golden gate pck, pgk, ATP synthase
  • Miniprepped better golden gate pck, pgk, ATP synthase (low concentration)


  • Ran sequencing PCR for pgk, pck (4 colonies each)
  • Gel Extraction for pgk, pck PCR product (no bands)


  • Ran sequencing PCR for pck and pgk on a gradient
  • Gel Extraction for pck PCR product was successful, not pgk


  • Ran sequencing PCR for pgk on a different gradient
  • Gel Extraction for pgk PCR product was not successful


No lab work done



  • Picked four new colonies of pgk
  • Started 4 new cultures from frozen stock


  • Made frozen stock of the 4 new colonies
  • Miniprepped all 8 pgk cultures and measured concentrations
  • Ran gradient PCR on all 8 pgk plasmids
  • Prepped and sent ATP synthase and pck in for sequencing


No lab work done



  • Mixed new pgk sequencing primers


  • Ran gradient PCR on all 8 pgk plasmids


No lab work done



No lab work done



No lab work done



4th of July!



No lab work done



  • Sequencing results for ATP and pck were not good
  • Reran seq PCR on ATP Synthase (bad results)
  • Picked new ATP colonies


  • Miniprepped overnight cultures of ATP 5-8 and forgot to make frozen stock
  • Ran PCR on ATP plasmids 5-8
  • PCR for pgk with new primers with gradient
  • Ran pgk PCR product on gels
  • Ran golden gate on pgk PCR gel extract


  • Electroporation with golden gate pgk product


  • Picked 4 pgk products and inoculated them overnight


  • Made frozen stock of new 4 pgk cultures
  • Miniprepped cultures
  • Seq PCR on new cultures
  • All 4 cultures showed correct bands
  • Clean and Concentrate pgk PCR product
  • Prepped pgk for sequencing


  • Delivered pgk for sequencing


  • New ATP synthase sequencing primers and pck regular primers came in
  • Seq PCR of ATP synthase plasmids with new sequencing primers. No bands
  • Picked 4 new ATP synthase colonies (again)
  • Inoculated MG1655 from frozens stock to make more genomic DNA


  • Extracted genomic DNA from MG1655
  • Miniprepped new ATP cultures
  • Ran sequencing PCR on new ATP and got no bands at all. Will use newly transformed cells tomorrow
  • Ran PCR with pck primers, got light bands
  • Reran PCR with pck products


  • Ran pck on gel
  • Gel extracted pck and combined gel extracts to improve concentration
  • Golden gate ligation on pck
  • Ran ATP sequencing PCR again with different backbone primers


  • Ran gel from yesterday’s ATP PCR. No bands
  • Ran PCR for pfo
  • Incubated pck and BioBrick cells


  • Miniprepped pck and biobrock genes (HSP)


No lab work done



  • Ran ATP seq PCR gradient again. No bands. Done with ATP
  • BioBrick digestion, ligation, transformation with HSP and GFP
  • Ran PCR seq for pck and gel extract


  • Delivered pck sequence to med school
  • Ligated backbone for BioBrick
  • Extracted more genomic DNA from MG1655
  • Gradient PCR on pfo
  • Received 𝛥aceE cells from Yale
  • Rehydrated and incubated 𝛥aceE cells


  • Ran PCR and gel extract for fldA backbone and petF backbone
  • DPn1 the two backbones
  • golden gate ligation on pfo gene and two backbones
  • Streaked colony from 𝛥aceE plate onto LB/kan plate


  • Making media for competent cell protocol
  • Made new LB with MgSO4 for chemical transformation


  • Digested BioBrick miniprep to check length


  • Chemically competent cell protocol with 𝛥aceE
  • Miniprepped pfo-petF and fldA-petF
  • Seq PCR miniprepped pfo-petF and fldA=petF
  • Remade LB with MgSO4 with correct concentration


No lab work done



  • Restarted comp cell protocol (3rd times the charm)
  • Clean and Concentrate junction 2 for pfo-fldA
  • Conducted HSP test in LB


  • Redid HSP test in minimal media M9
  • Recultured BioBrick and DH10B control


  • Reset up BioBrick experiment but grew diluted cells with LB instead of M9, so we have to do it again


  • Redid HSP test in minimal media M9


No lab work done



No lab work done



No lab work done



  • Chemical transformation of pfo-petF, pfo-fldA, petF, fldA into knockout strain, plated 80 and 240 ul, also control
  • Part 1 of interlab study (LUDOX)
  • Transforming 5 interlab plasmids


  • Picked and incubated electron donor colonies plated yesterday
  • Picked and incubated interlab colonies plated yesterday (2 per construct)


  • Made frozen stock of incubated colonies and interlab parts
  • Redid BioBrick experiment at 37C



No lab work done



No lab work done



No lab work done



  • Started induction but had to stop bc no frozen stock of fldA-pfo or petF-fldA in DH10B
  • Transformed fldA-pfo and petF-pfo into DH10B


  • Made frozen stock of 𝛥aceE
  • Made 60% ethanol
  • Incubated 8 constructs for biotin assay w/ methanol extraction (4 constructs and blank in DH10B and 𝛥aceE)


  • Methanol extraction assay (grew cells and froze pellets)


  • Assayed petF-aceE cells. Absorbance was too low to be meaningful


  • Ran preliminary sonication test
  • Incubated large volume of DH10B


  • Ran large sonication test on DH10B to decide protocol


  • Incubated large volume of DH10B


  • More sonication tests


  • Made ATP standards from assay instructions


  • Made more LB


  • Inoculated pgk, pck, DH10B for assay tomorrow


  • ATP luminescence assay
  • Inoculated 4 constructs and blank in 𝛥aceE cells for tomorrow


  • Nothing grew, couldn’t do induction


  • Inoculated DH10B constructs and control from frozen stock


  • DH10B electron donor induction, froze pellets, measured OD600
  • Re-transformed constructs into 𝛥aceE cells


  • Sonicated and assayed pellets for biotin (1 minute, 40 amp, 1mL PBS)
  • Reinoculated 𝛥aceE constructs in all antibiotic combinations to determine problem, in LB and plates


  • Visited Monsanto


  • Measured absorbance data from biotin in DH10B cells
  • Did ATP Assay and got luminescence data
  • Incubated all four 𝛥aceE electron donor constructs from frozen stock plates and original plates and incubated 𝛥aceE and DH10B


  • Nothing grew from yesterday's inoculations
  • Interlab study


  • Remade competent cells
  • Finished interlab study (FITC) and sent to iGEM HQ


No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



  • Picked colonies from 4 new 𝛥aceE plates and incubated


  • 𝛥aceE cell induction


No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



  • Incubated pck, pgk, DH10B from frozen stock


  • Ran ATP assay
  • BioBrick digestion and ligation
  • Sonicated 𝛥aceE pellets
  • Ran 𝛥aceE cell absorbance scan
  • Ran biotin assay on 𝛥aceE cells


No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



  • Transformed ligated petF and pck BioBrick plasmids into DH10B (wrong antibiotic)


No lab work done



  • Transformed ligated petF and pck BioBrick plasmids into DH10B (kan = right)
  • Wrapped pck and pgk plasmids to be sent to Cardiff University iGEM


  • Small colonies grew on all plates
  • Picked colonies and inoculated
  • Sent plasmids to Cardiff University iGEM


  • Miniprep on pck and petF BioBrick
  • Reusupended TetR-pTet gBlocks


  • Digestion/Ligation/Transformation of pSB1C3 and tetr-ptet BioBrick


  • TetR-pTet colonies grew, picked colonies


  • Miniprepped TetR-pTet BioBrick


  • digested BioBricks to check bands sizes


No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



No lab work done



  • Ran PCR on BioBrick plasmids


  • Ran PCR products on gel
  • Only petF showed up


  • digestion of pck, TetR-pTet, and pSB1C3 with EcoRI


  • Digestion of pck, TetR-pTet, and pSB1C3 with PstI
  • Ligated pck and TetR-pTet to pSB1C3
  • Transformed into DH10B


  • Only TetR-pTet grew
  • Ran colony PCR with 8 pck colonies and 8 TetR-pTet colonies


  • Ran colony PCR products on a gel but got no bands
  • Miniprepped pTet1-8
  • Ran PCR on pTet 1-8 plasmids to check if insert was present
  • Ran PCR product on gel, got the band!
  • Prepped petF, pck, and TetR-pTet to be sent to iGEM HQ


  • Last day in lab!
  • Sent parts to iGEM!


WIKIFREEZE



Done in lab



Prepping for Jamboree



Prepping for Jamboree



Prepping for Jamboree



Prepping for Jamboree



Prepping for Jamboree



Prepping for Jamboree



Fly to Boston for Jamboree



GIANT JAMBOREE



GIANT JAMBOREE



PRESENTATION



Fly back to St. Louis