Difference between revisions of "Team:UNSW Australia/Demonstrate"

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                <h2 style="font-family: Slim-Joe;">Demonstration of Hypervesiculation</h2>
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                <p>Our results showed that the knock-in mutation of g3p successfully induced hypervesiculation of E. coli with an OMV count of 2.8x108 per mL per OD600, significantly higher than the count obtained from the negative control sample (0.29x108 per mL per OD600) (Figure 1). Knock-out of TolA also showed an impressive increase in OMV production, over 36 times the amount in wildtype E. coli (Figure 1). This affect was all the more pronounced when TolR overexpression was added to this same knockout strain (Figure 1). Knockout of DegP also resulted in increased OMV production compared to the wildtype, nevertheless production fell very short of the production rates seen in the other mutants. Most importantly, however, knockout of DegP was associated with a dramatic increase in vesicle size, if not vesicle number (Figure 2).</p>
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                <p>Figure 1</p>
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                <img class="img-responsive" src="https://static.igem.org/mediawiki/parts/3/34/T--UNSW_Australia--ParticleCount.png">
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                <p>Figure 2</p>
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                <img class="img-responsive" src="https://static.igem.org/mediawiki/2016/1/14/T--UNSW_Australia--degp_results.png">
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Revision as of 03:23, 20 October 2016

Demonstration of Hypervesiculation

Our results showed that the knock-in mutation of g3p successfully induced hypervesiculation of E. coli with an OMV count of 2.8x108 per mL per OD600, significantly higher than the count obtained from the negative control sample (0.29x108 per mL per OD600) (Figure 1). Knock-out of TolA also showed an impressive increase in OMV production, over 36 times the amount in wildtype E. coli (Figure 1). This affect was all the more pronounced when TolR overexpression was added to this same knockout strain (Figure 1). Knockout of DegP also resulted in increased OMV production compared to the wildtype, nevertheless production fell very short of the production rates seen in the other mutants. Most importantly, however, knockout of DegP was associated with a dramatic increase in vesicle size, if not vesicle number (Figure 2).

Figure 1

Figure 2