Difference between revisions of "Team:Kyoto/Safety"

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{{Kyoto}}
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{{Kyoto_Common}}
 
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<head>
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<script type="text/javascript">
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<div class="column full_size">
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  }
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<ul>
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<li>Choosing a non-pathogenic chassis</li>
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  }
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<li>Choosing parts that will not harm humans / animals / plants</li>
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</script>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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</head>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<body>
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  <div id="background">
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    <img id="background-image" src="https://static.igem.org/mediawiki/2016/6/6f/T--Kyoto--pagephoto-03.png">
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  </div>
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    <div id="header">
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      <div id="nav-wrapper">
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        <div class="nav">
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          <ul class="nv">
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            <li id ="nv0" style="width:56px"><a href="https://2016.igem.org/Team:Kyoto"></a></li>
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            <li id ="nv1">
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              <a></a>
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              <ul>
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                <li id="nv11"><a href="https://2016.igem.org/Team:Kyoto/Description"></a></li>
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                <li id="nv12"><a href="https://2016.igem.org/Team:Kyoto/Proof"></a></li>
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              </ul>
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            </li>
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            <li id ="nv2">
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              <a></a>
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              <ul>
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                <li id="nv21"><a href="https://2016.igem.org/Team:Kyoto/Software"></a></li>
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                <li id="nv22"><a href="https://2016.igem.org/Team:Kyoto/Collaborations"></a></li>
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              </ul>
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            </li>
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            <li id ="nv3"><a href="https://2016.igem.org/Team:Kyoto/Achievements"></a></li>
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            <li id ="nv4"><a href="https://2016.igem.org/Team:Kyoto/Experiments"></a></li>
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            <li id ="nv5">
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              <a></a>
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              <ul>
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                <li id="nv51"><a href="https://2016.igem.org/Team:Kyoto/Integrated_Practices"></a></li>
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                <li id="nv52"><a href="https://2016.igem.org/Team:Kyoto/HP/Silver"></a></li>
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                <li id="nv53"><a href="https://2016.igem.org/Team:Kyoto/HP/Gold"></a></li>
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              </ul>
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            </li>
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            <li id ="nv6"><a href="https://2016.igem.org/Team:Kyoto/Notebook"></a></li>
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            <li id ="nv7">
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              <a></a>
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              <ul>
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                <li id="nv71"><a href="https://2016.igem.org/Team:Kyoto/Team"></a></li>
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                <li id="nv72"><a href="https://2016.igem.org/Team:Kyoto/Attributions"></a></li>
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              </ul>
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            </li>
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            <li id ="nv8"><a href="https://2016.igem.org/Team:Kyoto/Safety"></a></li>
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          </ul>
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        </div>
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      </div>
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    </div>
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    <div id="topbanner-wrapper">
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      </div>
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    </div>
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    <div id="contents-wrapper">
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  <div id="contents">
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<h6>Contents</h6>
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<ul id="cont">
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  <li><a href="#safe project design">1 Safe project design</a></li>
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  <li><a href="#safe lab work">2 Safe lab work</a></li>
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  <li><a href="#safe shipment">3 Safe shipment</a></li>
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  <li><a href="#link">4 Link</a></li>
 
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<h1 id="safe project design">1 Safe project design</h1>
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<li>BclA, a protein we have used for surface display, derives from <i>Bacillus anthracis</i> spore protein. <i>Bacillus anthracis</i> creates toxins in the human body, and it may cause severe illness. However, the gene coding for BclA protein is unrelated to the toxic genes. In addition, our BclA gene is chemically synthesized using DNA sequence data, and is not refined from <i>Bacillus anthracis</i> genome. </li>
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<h5>Safe Lab Work</h5>
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<li>We will sterilize <i>E. coli</i> before prescription as a medicine because the BclA structure does not require <i>E. coli</i> to be viable. This prevents genetically modified <i>E. coli</i> from propagating in the natural environment.</li>
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</ol>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h1 id="safe lab work">2 Safe lab work</h1>
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<ol>
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</div>
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<li>We did not use NoV in experiments for the assessment of scFv. Instead, we used NoV-like particles (NoVLP). They contain only the capsid proteins of the virus and lack the viral genome, thus not infectious.</li>
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<li>Three of our experimentation members have undergone a safety training session in Kyoto University Institute for Virus Research. They have shared their experiences with all our members. </li>
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<li>We have made our own lab safety and management manual.</li>
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</ol>
  
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<h1 id="safe shipment">3 Safe shipment</h1>
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<h5>Safe Shipment</h5>
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<ol>
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<li>Before submission, we have read all related pages in the iGEM web page. </li>
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<li>We have used submission kits and submitted our parts accordingly.</li>
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</ol>
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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<h1 id="link">4 Link</h1>
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</div>
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<ol>
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<li>Final Safety form</li>
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<a href="https://2016.igem.org/Safety/Final_Safety_Form?team_id=1933">https://2016.igem.org/Safety/Final_Safety_Form?team_id=1933</a>
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<li>Safety management manual of Kyoto University Graduate School of Science (Japanese)</li>
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<a href="https://static.igem.org/mediawiki/2016/6/6a/T--Kyoto--Safety.pdf">https://static.igem.org/mediawiki/2016/6/6a/T--Kyoto--Safety.pdf</a>
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</ol>
  
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      </div>
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  </div>
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    <div id="footer">
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    <div id="footer_logos">
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      <img src="https://static.igem.org/mediawiki/2016/e/e6/T--Kyoto--Footer-24.png" width="874" height="95">
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    <div id="footer_contact">
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    <div id="footer_social">
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      <a href="mailto:igemkyoto@gmail.com"><img src="https://static.igem.org/mediawiki/2016/2/28/T--Kyoto--Footer-28.png"></a>
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      <a href="https://twitter.com/igemkyoto"><img src="https://static.igem.org/mediawiki/2016/8/82/T--Kyoto--Footer-26.png"></a>
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      <a href="https://www.facebook.com/IgemKyoto/"><img src="https://static.igem.org/mediawiki/2016/a/a3/T--Kyoto--Footer-27.png"></a>
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    </div>
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Latest revision as of 01:05, 3 November 2016

Contents

1 Safe project design

  1. BclA, a protein we have used for surface display, derives from Bacillus anthracis spore protein. Bacillus anthracis creates toxins in the human body, and it may cause severe illness. However, the gene coding for BclA protein is unrelated to the toxic genes. In addition, our BclA gene is chemically synthesized using DNA sequence data, and is not refined from Bacillus anthracis genome.
  2. We will sterilize E. coli before prescription as a medicine because the BclA structure does not require E. coli to be viable. This prevents genetically modified E. coli from propagating in the natural environment.

2 Safe lab work

  1. We did not use NoV in experiments for the assessment of scFv. Instead, we used NoV-like particles (NoVLP). They contain only the capsid proteins of the virus and lack the viral genome, thus not infectious.
  2. Three of our experimentation members have undergone a safety training session in Kyoto University Institute for Virus Research. They have shared their experiences with all our members.
  3. We have made our own lab safety and management manual.

3 Safe shipment

  1. Before submission, we have read all related pages in the iGEM web page.
  2. We have used submission kits and submitted our parts accordingly.

4 Link

  1. Final Safety form
    2. https://2016.igem.org/Safety/Final_Safety_Form?team_id=1933
  2. Safety management manual of Kyoto University Graduate School of Science (Japanese)
    3. https://static.igem.org/mediawiki/2016/6/6a/T--Kyoto--Safety.pdf