Difference between revisions of "Team:BIT-China/E M"

Latest revision as of 11:16, 10 November 2016

What is the point?

Just show what things we use in the experiments.

Equipments

Name	Type	Company
Transferpettor	1000µl 100µl 10µl 2.5µl	Eppendorf
ELIASA	Elx808	America Bioteck company
Electrophresis Apparatus	DYY-6C	Beijing LIUYI bio-technology company
OD Machine	UV-2100	UNICO
Incubator Shaker	IS-RDS3	Beijing KECHUANGBAIFANG Technical Co., Ltd
Electro-heating Standing-temperature Water Bath	DK-8D	Shanghai YIHENG Technical Co., Ltd
Gel-imaging System	GeneGenius	SYNGENE
Blue Rubber Cutting Machine	BD-BGCI	Biofriend
Electro-heating Standing-temperature Cultivator	SKP-02.420	America HENGFENG company
High Speed Freezing Centrifuge	D-37520	Germany Thermo company
Low temperature enzyme even instrument	L1	HEMA
Electronic Balance	FA2104SW	MINQIAO
High-Pressure Steam Sterilization Pot	MLS-3750	SANYO
Laminar Flow Cabinet	SW-CJ-2FD	Taisite
Microwave（微波炉）		Galanz
Electro-heating Standing-temperature Drum Wind Drying Machine	DHG-9030A	Shanghai YIHENG Technical Co., Ltd
96 Well Thermal Cycler（PCR仪）		Gene Company Limited
High Speed Micro-Centrifuge	D3024	SCILOGEX
Refrigerator	BCD-610W	SIEMENS
Heto Ultra Freeze(-80℃ 冰箱)		Thermo
Ultrapure Water System（超纯水系统）
Ice Machine	SPR-120	SIMAG
Nano Drop	2000c	America Thermo Scientific company
Microscope（显微镜）		Ruich Allway
Noise Isolating Tamber	vcx750	Ningbo Scientz Biotechnology CO. , Ltd
Noise Cleaner	KQ-100DE	Kunshang Noise Equipment CO. Ltd.

Reagent & Material

1. antibiotic

Ampicillin: dissolved in distilled water and the final concentration is 100mg/ml

Chloromycetin : dissolved in absolute ethyl alcohol and final concentration is 25 mg/ml

Kanamycin: dissolved by distilled water and final concentration is 50 mg/ml

2. Maker

Trans 2K DNA Maker

Trans 2K plus DNA Maker

Trans 1KB DNA Maker

Trans 2K plusⅡDNA Maker

All from Trans Gen Biotech

3. Buffer

Buffer	From
6XDNA Loading buffer	Trans Gen Biotech
2×Primer star CG buffer	TaKaRa
2×Taq PCR Master Mix	Biomed
2×ES Taq Master Mix	CWBio
10×T4 DNA Ligase Buffer	TaKaRa
10×Fast Digest Green Buffer	Scientific
10×Fast Digest Buffer	Scientific
10×Pfu Buffer	Biomed
5×Trans Start FastPfu Buffer	Trans Gen Biotech
5×FastPfu Fly Buffer	Trans Gen Biotech

4.

10% glycerol: 12.6g glycerol dissolved in 90 ml of water.

50%glycerol: use distilled water to dilute 50ml glycerol twice as much

5. dd H2O

6. Enzyme

Enzyme	From
T4 DNA Ligase	Thermo Scientific
Primer star HS DNA polymerase	TaKaRa
Pfu DNA polymerase	Biomed
Trans Start FastPfu DNA polymerase	Trans Gen Biotech
Trans Start FastPfu Fly DNA polymerase	Trans Gen Biotech
Fast Digest DpnⅠ	Thermo Scientific
Fast digest EcoRⅠ	Thermo Scientific
Fast digest PstⅠ	Thermo Scientific
Fast digest XbaⅠ	Thermo Scientific
Fast digest SpeⅠ	Thermo Scientific

7. absolute ethyl alcohol

8. Kit

TIANgel Midi Purification Kit

TIANprep Mini plasmid Kit

TIANamp Bacteria DNA Kit

All from TIANGEN BIOTECH (BEIJING) CO.LTD

9. Agarose Gel Electrophoresis:

Golden View from Biomed

BIOWEST REGULAR AGAROSE G-10

1XTAE：Diluted with 50Xtae

50XTAE: Tris 242g，Na2EDTA·2H2O 37.2g , 57.1ml glacial acetic acid,1L water

10. Culture medium

LB: 10g Tryptone peptone,5g Yeast extract, 5gNaCl; dissolve in 1.0L water.

SOB:2.0g Tryptone peptone,0.5g Yeast extract,0.2ml 5M NaCl,0.25ml 1M KCL; dissolve in 90 ml water. Adjust PH to 7.0.Bring volume to 100 ml. Autoclave. Add 1.0 ml sterile 1 M MgCl2 and 1.0 ml sterile 1 M MgSO4.

11. Arabinose

1 M Arabinose: weight 150g then use distilled water to dilute it

10% Arabinose: use distilled water to dilute 1M Arabinose

12. IPTG: confect the final concentration of 1M IPTG with distilled water

13. High pure dNTPs from Trans Gen Biotech

14. Bacteria

TOP10 from Biomed

DH5α from Biomed

15. Golden View from Biomed

E & M

Equipments
Materials

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