Difference between revisions of "Team:BIT-China/Safety"

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                <div class="content-title col-sm-12">
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                    <img src="https://static.igem.org/mediawiki/2016/a/ab/T--BIT-China--Notebook--Safety--title.png"
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                </div>
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                <div class="problem-txt col-sm-12 block-content block-header-margin1">
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                    Every member of our team has been trained about general lab safety and project design safety by our previous members. For example, the former leader and our advisors taught us how to run the experiment correctly and avoid mistakes.
 +
                </div>
 +
 +
                <!--Project design safety-->
 +
                <div id="design_safety" class="block-title">Project design safety</div>
 +
                <div class="block-content">
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                    <div class="block-content-item">
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                        <div class="block-content-item-block">
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                            <div>
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                                For the safety of our team members, we designed the project to be as safe as possible. Because the core of our project is  allowing bacteria to commit suicide. Considering biosafety, firstly we selected Escherichia coli as the host cell when implementing the project. To avoid the number of pathogenicity, we limited its number under a specific range. Second, we selected the toxic protein which does no harm to human body. Third, in the process of implementation of the project we chose the RFP as a reporter gene which can be detected directly without adding any harmful reagents. At last, we even used inducible promoters to control the expression of toxin protein genes in gene circuits.
 +
                            </div>
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
 +
                <!--Lab Safety-->
 +
                <div id="lab_safety" class="block-title">Lab Safety</div>
 +
                <div class="block-content">
 +
                    <div class="block-content-item">
 +
                        <div class="block-content-item-block">
 +
                            <div>
 +
                                Our team conducted research in a safety level 1/2 laboratory. The organisms we used, E. coli strain DH5α and E. coli Top 10 are suitable for the S1/2-safety laboratory we are working in. Besides, the plasmids we are using for transformation (pSB1C3, pKD46, pKD3, pSB3K3, pSB1K3, pSB4K5 for E. coli) are not harmful for humans.
 +
                            </div>
 +
                            <div class="block-paragraph">
 +
                                See more in
 +
                                <a href="https://2016.igem.org/Team:BIT-China/Notebook" style="color: blue;">Notebook</a>/<a href="https://2016.igem.org/Team:BIT-China/E_M" style="color: blue;">Equipments & Materials</a>
 +
                            </div>
 +
                            <div class="block-paragraph">
 +
                                Because of the nature of our project,  contacting with microorganisms is inevitable. In order to avoid any harmful effects to each member of our team, all laboratory work was in accordance with the safety requirements for the laboratory. This includes, but is not limited to, the following examples:
 +
                            </div>
 +
                            <div class="block-paragraph-padding">
 +
                                ① Eating, drinking and smoking are prohibited in the laboratory.
 +
                            </div>
 +
                            <div class="block-paragraph-padding">
 +
                                ② Don’t deal with the wastes at random.
 +
                            </div>
 +
                            <div class="block-paragraph-padding">
 +
                                ③ All experimenter must wear protective equipment such as gloves and Lab coat.
 +
                            </div>
 +
                            <div class="block-paragraph-padding">
 +
                                ④ All equipments that came into contact with biological material (such as tips and glass glassware) must be autoclaved.
 +
                            </div>
 +
                            <div class="block-paragraph-padding">
 +
                                ⑤ Store quick-burning, flammable materials in designated locations which are away from ignition sources for fire protection. And there is no accumulated clutter in the hallway, for our safety while accident happening.
 +
                            </div>
 +
                            <div class="block-paragraph-padding">
 +
                                ⑥ Laboratory for experiments, seminar rooms for research and discussion, and the office used tosearch for information and analyze data are rigorously divided. These provisions are intended to prevent the possibility of unnecessary cross-contamination in the laboratory.
 +
                            </div>
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
 +
                <!--Dangerous Safety-->
 +
                <div id="dangerous" class="block-title">Dangerous chemicals</div>
 +
                <div class="block-content">
 +
                    <div class="block-content-item">
 +
                        <div class="block-content-item-block">
 +
                            We tried to avoid the use of dangerous chemicals whenever possible. We have completely banned a number of dangerous chemicals, such as ethidium bromide which has been replaced by anthocyanin for our DNA gel electrophoresis. At the same time, we have restricted the use of dangerous chemicals (such as acrylamide) which must be clearly placed in marked areas in the laboratory. And we are also required to keep the chemistry reagents in classified storage.
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
 +
                <div class="reference block-content" style="margin-top: 20px">
 +
                    <div class="block-red">
 +
                        This is the standard of the Chinese laboratory website:
 +
                    </div>
 +
                    <div>
 +
                        <a href="http://www.gov.cn/zwgk/2005-05/23/content_256.htm" style="color: blue;"></a>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
        </div>
 +
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 +
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    <div class="slow-transition nav-left" id="nav_left_wrapper">
 +
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 +
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 +
            <span style="color: #FFFFFF;font-size: 20px;font-weight: bold;position: absolute;
 +
        right: 40px;bottom: 20px;">Safety</span>
 +
        </div>
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 +
            <li>
 +
                <img src="https://static.igem.org/mediawiki/2016/e/e2/T--BIT-China--img--nav_left_c1.png"
 +
                    alt="c1" style="width: 100%;height: 70px;">
 +
                <a href="#design_safety" class="nav_point">
 +
                    <span class="nav_left_txt" style="font-size: 0.8em">Project design safety</span></a>
 +
            </li>
 +
            <li>
 +
                <img src="https://static.igem.org/mediawiki/2016/a/a1/T--BIT-China--img--nav_left_c2.png"
 +
                    alt="c1" style="width: 100%;height: 70px;">
 +
                <a href="#lab_safety" class="nav_point">
 +
                    <span class="nav_left_txt">Lab Safety</span></a>
 +
            </li>
 +
            <li>
 +
                <img src="https://static.igem.org/mediawiki/2016/9/9d/T--BIT-China--img--nav_left_c3.png"
 +
                    alt="c1" style="width: 100%;height: 70px;">
 +
                <a href="#dangerous" class="nav_point">
 +
                    <span class="nav_left_txt" style="font-size: 0.8em" >Dangerous chemicals</span></a>
 +
            </li>
 +
        </ul>
 +
    </div>
 +
 +
    <!--相邻页面切换按钮-->
 +
    <div class="slow-transition" id="site-change-btn"
 +
        style="position: absolute;left: 60px;bottom: 20px;z-index:9;display: none;">
 +
        <a href="https://2016.igem.org/Team:BIT-China/E_M">
 +
        <span style="color: #654D6F;font-weight: bold;font-size: 12px">
 +
            <i class="fa fa-angle-double-left" aria-hidden="true"></i>E & M</span>
 +
        </a>
 +
        <img src="https://static.igem.org/mediawiki/2016/a/ab/T--BIT-China--img--page_change_left.png"
 +
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 +
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 +
</div>
 +
 +
 
</body>
 
</body>
 
</html>
 
</html>
 
{{BIT-China/footer}}
 
{{BIT-China/footer}}

Latest revision as of 11:17, 10 November 2016

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Every member of our team has been trained about general lab safety and project design safety by our previous members. For example, the former leader and our advisors taught us how to run the experiment correctly and avoid mistakes.
Project design safety
For the safety of our team members, we designed the project to be as safe as possible. Because the core of our project is allowing bacteria to commit suicide. Considering biosafety, firstly we selected Escherichia coli as the host cell when implementing the project. To avoid the number of pathogenicity, we limited its number under a specific range. Second, we selected the toxic protein which does no harm to human body. Third, in the process of implementation of the project we chose the RFP as a reporter gene which can be detected directly without adding any harmful reagents. At last, we even used inducible promoters to control the expression of toxin protein genes in gene circuits.
Lab Safety
Our team conducted research in a safety level 1/2 laboratory. The organisms we used, E. coli strain DH5α and E. coli Top 10 are suitable for the S1/2-safety laboratory we are working in. Besides, the plasmids we are using for transformation (pSB1C3, pKD46, pKD3, pSB3K3, pSB1K3, pSB4K5 for E. coli) are not harmful for humans.
See more in Notebook/Equipments & Materials
Because of the nature of our project, contacting with microorganisms is inevitable. In order to avoid any harmful effects to each member of our team, all laboratory work was in accordance with the safety requirements for the laboratory. This includes, but is not limited to, the following examples:
① Eating, drinking and smoking are prohibited in the laboratory.
② Don’t deal with the wastes at random.
③ All experimenter must wear protective equipment such as gloves and Lab coat.
④ All equipments that came into contact with biological material (such as tips and glass glassware) must be autoclaved.
⑤ Store quick-burning, flammable materials in designated locations which are away from ignition sources for fire protection. And there is no accumulated clutter in the hallway, for our safety while accident happening.
⑥ Laboratory for experiments, seminar rooms for research and discussion, and the office used tosearch for information and analyze data are rigorously divided. These provisions are intended to prevent the possibility of unnecessary cross-contamination in the laboratory.
Dangerous chemicals
We tried to avoid the use of dangerous chemicals whenever possible. We have completely banned a number of dangerous chemicals, such as ethidium bromide which has been replaced by anthocyanin for our DNA gel electrophoresis. At the same time, we have restricted the use of dangerous chemicals (such as acrylamide) which must be clearly placed in marked areas in the laboratory. And we are also required to keep the chemistry reagents in classified storage.
This is the standard of the Chinese laboratory website:

P-SLACKiller

· Work or Die
· Monitor Plasmid Numbers
· Kill the Slacker
· Maintain the Better Biosystem

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