Difference between revisions of "Team:NYU-AD"

 
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<li><a href="https://2016.igem.org/Team:NYU-AD/Design">Design</a></li>
 
<li><a href="https://2016.igem.org/Team:NYU-AD/Design">Design</a></li>
 
<li><a href="https://2016.igem.org/Team:NYU-AD/Experiments">Experiments</a></li>
 
<li><a href="https://2016.igem.org/Team:NYU-AD/Experiments">Experiments</a></li>
<li><a href="https://2016.igem.org/Team:NYU-AD/Proof">Proof of Concept</a></li>
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<li><a href="https://2016.igem.org/Team:NYU-AD/Demonstrate">Demonstrate</a></li>
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<li><a href="https://2016.igem.org/Team:NYU-AD/Results">Results</a></li>
 
<li><a href="https://2016.igem.org/Team:NYU-AD/Results">Results</a></li>
 
<li><a href="https://2016.igem.org/Team:NYU-AD/Notebook">Notebook</a></li>
 
<li><a href="https://2016.igem.org/Team:NYU-AD/Notebook">Notebook</a></li>
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</li>
 
</li>
 
<li class="dropdown">
 
<li class="dropdown">
<a class="dropdown-toggle" data-toggle="dropdown" href="#">PARTS<span class="caret"></span></a>
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<a class="dropdown-toggle" data-toggle="dropdown" href="#">HUMAN PRACTICES<span class="caret"></span></a>
 
<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
<li><a href="https://2016.igem.org/Team:NYU-AD/Parts">Parts</a></li>
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<li><a href="https://2016.igem.org/Team:NYU-AD/Human_Practices">Human Practices</a></li>
<li><a href="https://2016.igem.org/Team:NYU-AD/Basic_Part">Basic Parts</a></li>
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<li><a href="https://2016.igem.org/Team:NYU-AD/HP/Silver">Silver Criterion</a></li>
<li><a href="https://2016.igem.org/Team:NYU-AD/Composite_Part">Composite Parts</a></li>
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<li><a href="https://2016.igem.org/Team:NYU-AD/HP/Gold">Gold Criterion</a></li>
<li><a href="https://2016.igem.org/Team:NYU-AD/Part_Collection">Part Collection</a></li>
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<li><a href="https://2016.igem.org/Team:NYU-AD/Integrated_Practices">Integrated Practices</a></li>
 
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<a class="dropdown-toggle" data-toggle="dropdown" href="#">HUMAN PRACTICES<span class="caret"></span></a>
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<li><a href="https://2016.igem.org/Team:NYU-AD/Human_Practices">Human Practices</a></li>
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<li><a href="https://2016.igem.org/Team:NYU-AD/HP/Silver">Silver</a></li>
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<li><a href="https://2016.igem.org/Team:NYU-AD/HP/Gold">Gold</a></li>
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<li><a href="https://2016.igem.org/Team:NYU-AD/Integrated_Practices">Integrated Practices</a></li>
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           <h1 id="project-abstract-heading">Project Abstract</h1>
 
           <h1 id="project-abstract-heading">Project Abstract</h1>
           <p id="abstract-para">In many developing countries people depend on reasonably priced and conveniently available street food. However, lack of action taken by governments to regulate street food vendors has lead to the prevalence of severe street food-related illnesses. One of the primary microbial contaminants in street food is E. coli O157:H7, which acts by secreting Shiga-like toxin (SLT). Currently, there is no detection method for SLT outside of a lab setting, thus putting the consumers of foods at risk. Our project aims to develop a device that would remain in the stall or restaurant and be used by the vendors themselves to verify the safety of their food. Our device focuses on detecting the interaction between the Gb3 receptor, produced by E. coli Gb3 synthase, and the non-toxic component of SLT, Subunit B. The performance of SDS-PAGE will allow consumers to compare the migration patterns to positive and negative controls and identify the presence of SLT.</p>
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           <p id="abstract-para">In many developing countries, people depend on reasonably priced and conveniently available street food. However, lack of action taken by governments to regulate street food vendors has led to the prevalence of severe street food-related illnesses. One of the primary microbial contaminants in street food is <i>E. coli</i> O157:H7, which acts by secreting the Shiga-like toxin (SLT). Currently, there is no detection method for SLT outside of a lab setting, thus putting the consumers of foods at risk. Our project aims to develop a device that would be used by street vendors and restaurant owners to verify the safety of their products. Through our device, we exploit the binding of Gb3 to subunit B of the Shiga toxin, and compare the migration pattern of the bound Gb3-subunit B complex to a non bound subunit B. A shift in the migration pattern on a PAGE gel will occur when Gb3 is bound, indicating the presence of the toxin in the food sample. If no shift occurs in the SLT migration pattern, this implies the absence of the toxin within the sample, and reflects the safety status of the food.</p>
  
 
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             <h2 class="section-title"><span>Interested ? We can tell you more about</span></h2>
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             <h2 class="section-title"><span>Interested? Find out more about our...</span></h2>
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                 <h4>Project Description</h4>
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                 <h4>Project Description</h4></a>
 
              
 
              
 
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                 <h4>Human Practices</h4></a>
 
                
 
                
 
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                 <h4>Team</h4></a>
 
                
 
                
 
               </div>
 
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Latest revision as of 03:36, 3 December 2016

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Project Abstract

In many developing countries, people depend on reasonably priced and conveniently available street food. However, lack of action taken by governments to regulate street food vendors has led to the prevalence of severe street food-related illnesses. One of the primary microbial contaminants in street food is E. coli O157:H7, which acts by secreting the Shiga-like toxin (SLT). Currently, there is no detection method for SLT outside of a lab setting, thus putting the consumers of foods at risk. Our project aims to develop a device that would be used by street vendors and restaurant owners to verify the safety of their products. Through our device, we exploit the binding of Gb3 to subunit B of the Shiga toxin, and compare the migration pattern of the bound Gb3-subunit B complex to a non bound subunit B. A shift in the migration pattern on a PAGE gel will occur when Gb3 is bound, indicating the presence of the toxin in the food sample. If no shift occurs in the SLT migration pattern, this implies the absence of the toxin within the sample, and reflects the safety status of the food.



Interested? Find out more about our...