-
Take from the glycerinates of Goldenbraid Collection:
Plasmid GB Code pD6B3 α1 GB0015 pD6B3 α2 GB0017 pD6B3 Ω1 GB0019 pD6B3 Ω2 GB0021 pUPD2 GB0307 -
Prepare liquid culture (3 mL LB + 3 μL antibiotic) 1:1000. Incubate at 37°C overnight.
- Experiment with snails:
-
Let both N. benthamianaleafs with snails overnight at room temperature in separated boxes. We will observe if snails eat the leafs and if appears fluorescence.
-
Difference between revisions of "Team:Valencia UPV/HP/Silver"
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| + | <h1>May</h1> | ||
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<p class="post">Agroinfiltration in <i>Nicotiana benthamiana</i> of C58 with dsRED. <a href="" target="blank"></a></p> | <p class="post">Agroinfiltration in <i>Nicotiana benthamiana</i> of C58 with dsRED. <a href="" target="blank"></a></p> | ||
</div> | </div> | ||
| + | </div> | ||
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| + | |||
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| + | <h1>June</h1> | ||
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</ul> | </ul> | ||
<div class="post"> | <div class="post"> | ||
| − | + | <ul> | |
| − | + | <li> | |
| − | + | <p>Take from the glycerinates of Goldenbraid Collection: </p> | |
| − | + | <div class="table-responsive"> | |
| − | + | <table class="table table-striped"> | |
| − | + | <thead> | |
| − | + | <tr> | |
| − | + | <th>Plasmid</th> | |
| − | + | <th>GB Code</th> | |
| − | + | </tr> | |
| − | + | </thead> | |
| − | + | <tbody> | |
| − | + | <tr> | |
| − | + | <td>pD6B3 α1</td> | |
| − | + | <td>GB0015</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>pD6B3 α2</td> | |
| − | + | <td>GB0017</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>pD6B3 Ω1</td> | |
| − | + | <td>GB0019</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>pD6B3 Ω2</td> | |
| − | + | <td>GB0021</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>pUPD2</td> | |
| − | + | <td>GB0307</td> | |
| − | + | </tr> | |
| − | + | </tbody> | |
| − | + | </table> | |
| − | + | </div> | |
| − | + | </li> | |
| − | + | <li> | |
| − | + | <p>Prepare liquid culture (3 mL LB + 3 μL antibiotic) 1:1000. Incubate at 37°C overnight.</p> | |
| − | + | </li> | |
| − | + | <li>Experiment with snails: | |
| − | + | <ul> | |
| − | + | <li> | |
| − | + | <p>Let both <i>N. benthamiana</i>leafs with snails overnight at room temperature in separated boxes. We will observe if snails eat the leafs and if appears fluorescence.</p> | |
| − | + | </li> | |
| − | + | </ul> | |
| − | + | </li> | |
| − | + | </ul> | |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | + | </div> | |
</div> | </div> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 14:25, 1 August 2016
May
May 18, 2016
Take glycerinated cultures of C58 Agrobacterium with dsRED from GoldenBraid Collection. Prepare broth culture (5 mL LB + 5 μL kanamycin + 5 μL Rifampin) 1:1000 and incubate overnight at 28°C.
May 19, 2016
Refresh previously made culture by inoculating 10 μL in a new culture medium.
