Difference between revisions of "Team:Valencia UPV/HP/Silver"

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  <h2>June 6, 2016</h2>
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       <h1>May</h1>
 
       <h1>May</h1>
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      <h2>May 18, 2016</h2>
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        <h2>May 18, 2016</h2>
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      <p class="post">Take glycerinated cultures of C58 <i>Agrobacterium</i> with dsRED from GoldenBraid Collection. Prepare broth culture (5 mL LB + 5 μL kanamycin + 5 μL Rifampin) 1:1000 and incubate overnight at 28°C.</p>
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        <p class="post">Take glycerinated cultures of C58 <i>Agrobacterium</i> with dsRED from GoldenBraid Collection. Prepare broth culture (5 mL LB + 5 μL kanamycin + 5 μL Rifampin) 1:1000 and incubate overnight at 28°C.</p>
 
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      <h2>May 19, 2016</h2>
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        <h2>May 19, 2016</h2>
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      <p class="post">Refresh previously made culture by inoculating 10 μL in a new culture medium.</p>
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        <p class="post">Refresh previously made culture by inoculating 10 μL in a new culture medium.</p>
 
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      <h2>May 20, 2016</h2>
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        <h2>May 20, 2016</h2>
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      <p class="post">Agroinfiltration in <i>Nicotiana benthamiana</i> of C58 with dsRED.
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        <p class="post">Agroinfiltration in <i>Nicotiana benthamiana</i> of C58 with dsRED.
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       <h1>June</h1>
 
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      <h2>June 6, 2016</h2>
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        <h2>June 6, 2016</h2>
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          <p>Take from the glycerinates of Goldenbraid Collection: </p>
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          <table class="table table-striped">
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            <thead>
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            <tr>
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              <th>Plasmid</th>
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              <th>GB Code</th>
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            </tr>
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            </thead>
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            <tbody>
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            <tr>
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              <td>pD6B3 α1</td>
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              <td>GB0015</td>
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            </tr>
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            <tr>
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              <td>pD6B3 α2</td>
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              <td>GB0017</td>
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            </tr>
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            <tr>
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              <td>pD6B3 Ω1</td>
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              <td>GB0019</td>
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            </tr>
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            <tr>
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              <td>pD6B3 Ω2</td>
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              <td>GB0021</td>
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            </tr>
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            <tr>
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              <td>pUPD2</td>
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              <td>GB0307</td>
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            </tbody>
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          </table>
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        </li>
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        <li>
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          <p>Prepare liquid culture (3 mL LB + 3 μL antibiotic) 1:1000. Incubate at 37°C overnight.</p>
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        </li>
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        <li>Experiment with snails:
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           <ul>
 
           <ul>
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          <li>
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            <li>
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             <p>Let both <i>N. benthamiana</i>leafs with snails overnight at room temperature in separated boxes. We will observe if snails eat the leafs and if appears fluorescence.</p>
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              <p>Take from the glycerinates of Goldenbraid Collection: </p>
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          </li>
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              <div class="table-responsive">
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                <table class="table table-striped">
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                  <thead>
 +
                    <tr>
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                      <th>Plasmid</th>
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                      <th>GB Code</th>
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                    </tr>
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                  </thead>
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                  <tbody>
 +
                    <tr>
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                      <td>pD6B3 α1</td>
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                      <td>GB0015</td>
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                    </tr>
 +
                    <tr>
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                      <td>pD6B3 α2</td>
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                      <td>GB0017</td>
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                    </tr>
 +
                    <tr>
 +
                      <td>pD6B3 Ω1</td>
 +
                      <td>GB0019</td>
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                    </tr>
 +
                    <tr>
 +
                      <td>pD6B3 Ω2</td>
 +
                      <td>GB0021</td>
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                    </tr>
 +
                    <tr>
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                      <td>pUPD2</td>
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                      <td>GB0307</td>
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                    </tr>
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                  </tbody>
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                </table>
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              </div>
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             </li>
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            <li>
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              <p>Prepare liquid culture (3 mL LB + 3 μL antibiotic) 1:1000. Incubate at 37°C overnight.</p>
 +
            </li>
 +
            <li>Experiment with snails:
 +
              <ul>
 +
                <li>
 +
                  <p>Let both <i>N. benthamiana</i>leafs with snails overnight at room temperature in separated boxes. We will observe if snails eat the leafs and if appears fluorescence.</p>
 +
                </li>
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Revision as of 14:39, 1 August 2016

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May

May 18, 2016

Take glycerinated cultures of C58 Agrobacterium with dsRED from GoldenBraid Collection. Prepare broth culture (5 mL LB + 5 μL kanamycin + 5 μL Rifampin) 1:1000 and incubate overnight at 28°C.

May 19, 2016

Refresh previously made culture by inoculating 10 μL in a new culture medium.

May 20, 2016

Agroinfiltration in Nicotiana benthamiana of C58 with dsRED.

June

June 6, 2016

  • Take from the glycerinates of Goldenbraid Collection:

    Plasmid GB Code
    pD6B3 α1 GB0015
    pD6B3 α2 GB0017
    pD6B3 Ω1 GB0019
    pD6B3 Ω2 GB0021
    pUPD2 GB0307

  • Prepare liquid culture (3 mL LB + 3 μL antibiotic) 1:1000. Incubate at 37°C overnight.

  • Experiment with snails:

    • Let both N. benthamianaleafs with snails overnight at room temperature in separated boxes. We will observe if snails eat the leafs and if appears fluorescence.