Difference between revisions of "Team:UCLA/Notebook"

Line 46: Line 46:
 
<li>X90 w/ EC869 cosmid</li>
 
<li>X90 w/ EC869 cosmid</li>
 
<li>ATCC 13048</li>
 
<li>ATCC 13048</li>
 +
</ul>
 +
<p> Conditions: </p>
 +
<ul>
 +
<li>10mL LB</li>
 +
<li>10uL ampicillin</li>
 +
<li>37 C for 16h shaking</li>
 
</ul>
 
</ul>
 
       </div>
 
       </div>

Revision as of 21:57, 9 August 2016

JOURNAL

Projects

CDI

Protein Cages

Week

1

Construction of ATCC 13048 Loci 2 in pSB1C3

Isolation of template DNA from EC93, EC869, and ATCC 13048; PCR of Gibson fragments for EBL2 in pSB1C3 construct

Jun

27

Inoculation of strains obtained from Dr. Christopher Hayes

Inoculated overnight cultures of:

  • EPI100 w/ EC93 cosmid
  • X90 w/ EC869 cosmid
  • ATCC 13048

Conditions:

  • 10mL LB
  • 10uL ampicillin
  • 37 C for 16h shaking
Aug

5

Cloning Experiments

here's the day entry

Week

1

Title For The Week

Description blah blah blah

Jun

24

Title for the day (e.g. "Cloning Mutants")

Today we did cloning. fuck cloning. blah blah blah.