Difference between revisions of "Team:UCLA/Notebook"

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<li>ATCC 13048</li>
 
<li>ATCC 13048</li>
 
</ul>
 
</ul>
 +
 
<p> Conditions: </p>  
 
<p> Conditions: </p>  
 
<ul>
 
<ul>
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     <div class="row day-entry">
 
     <div class="row day-entry">
 
       <div class="columns small-2 small-offset-1 day-date">
 
       <div class="columns small-2 small-offset-1 day-date">
−
         <span>Aug</span>
+
         <span>Jun</span>
−
         <p>5</p>
+
         <p>28</p>
 
       </div>
 
       </div>
 
       <div class="columns small-9">
 
       <div class="columns small-9">
−
         <h5>Cloning Experiments</h5>
+
         <h5>Isolation of template DNA</h5>
−
         <p>here's the day entry
+
         <p> Miniprep of EC93 and EC869 </p>
−
          <img src="http://www.google.com">
+
      </div>
−
          </p>
+
        <ul>
 +
          <li>EC93 #1: 145.42 ng/μL</li>
 +
          <li>EC93 #2: 198.85 ng/μL</li>
 
       </div>
 
       </div>
 
     </div>
 
     </div>

Revision as of 22:01, 9 August 2016

JOURNAL

Projects

CDI

Protein Cages

Week

1

Construction of ATCC 13048 Loci 2 in pSB1C3

Isolation of template DNA from EC93, EC869, and ATCC 13048; PCR of Gibson fragments for EBL2 in pSB1C3 construct

Jun

27

Inoculation of strains obtained from Dr. Christopher Hayes

Inoculated overnight cultures of:

  • EPI100 w/ EC93 cosmid
  • X90 w/ EC869 cosmid
  • ATCC 13048

Conditions:

  • 10mL LB
  • 10uL ampicillin
  • 37 C for 16h shaking
Jun

28

Isolation of template DNA

Miniprep of EC93 and EC869

  • EC93 #1: 145.42 ng/μL
  • EC93 #2: 198.85 ng/μL
Week

1

Title For The Week

Description blah blah blah

Jun

24

Title for the day (e.g. "Cloning Mutants")

Today we did cloning. fuck cloning. blah blah blah.