Difference between revisions of "Team:Cambridge-JIC/Description"

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<h1>Background</h1>
 
<h1>Background</h1>
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blah blah blah blah
  
 
<h3>Why use <i>Chlamydomonas Reinhardtii</i>?</h3>
 
<h3>Why use <i>Chlamydomonas Reinhardtii</i>?</h3>
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<li>Fast growing</li>
 
<li>Fast growing</li>
 
<li>Very efficient homologous recombination in chloroplast</li>
 
<li>Very efficient homologous recombination in chloroplast</li>
 +
<li>Conveniently only has one (quite large) chloroplast </li>
 
</ul>
 
</ul>
 +
 +
<h3>Why Chloroplasts?</h3>
 +
<p>We chose chloroplasts because they're fun and quite nice to us. They're also the home of photosynthesis -- the most interesting part of the cell, and are able to churn out huge amounts of proteins. </p>
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 +
<h1>A Toolbox for Chloroplast Transformation</h1>
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 +
<p>Our toolbox aims to:</p>
 +
<ul>
 +
<li>Produce a standard method for the chloroplast transformation</li>
 +
<li>Attempt to use self-expressing cas9 in the chloroplast, in order to achieve ploidy.</li>
 +
<li>Provide a facility in which to grow Chalamydomonas </li>
 +
<li>Provide an affordable biolistics design for smaller labs </li>
 +
</ul>
 +
 +
  
  

Revision as of 15:45, 9 September 2016

Cambridge-JIC

Background

blah blah blah blah

Why use Chlamydomonas Reinhardtii?

We intend to use Chlamydomonas Reinhardtii as a simple model to represent higher order systems.

There are many reasons why one would want to chose Chlamydomonas Reinhardtii:

  • Chlamydomonas reinhardtii is a unicellular alga which is a relatively well established model organism and many techniques can be readily transferred to higher plants.
  • It is registered as safe to humans so there is a lot of interest in developing edible vaccines and other foodstuffs.
  • Fast growing
  • Very efficient homologous recombination in chloroplast
  • Conveniently only has one (quite large) chloroplast

Why Chloroplasts?

We chose chloroplasts because they're fun and quite nice to us. They're also the home of photosynthesis -- the most interesting part of the cell, and are able to churn out huge amounts of proteins.

A Toolbox for Chloroplast Transformation

Our toolbox aims to:

  • Produce a standard method for the chloroplast transformation
  • Attempt to use self-expressing cas9 in the chloroplast, in order to achieve ploidy.
  • Provide a facility in which to grow Chalamydomonas
  • Provide an affordable biolistics design for smaller labs