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Revision as of 21:34, 16 September 2016

Cambridge-JIC

Background

blah blah blah blah

Why use Chlamydomonas Reinhardtii?

We intend to use Chlamydomonas Reinhardtii as a simple model to represent higher order systems.

There are many reasons why one would want to chose Chlamydomonas Reinhardtii:

  • Chlamydomonas reinhardtii is a unicellular alga which is a relatively well established model organism and many techniques can be readily transferred to higher plants.
  • It is registered as safe to humans so there is a lot of interest in developing edible vaccines and other foodstuffs.
  • Fast growing
  • Very efficient homologous recombination in chloroplast
  • Conveniently only has one (quite large) chloroplast

Why Chloroplasts?

We chose chloroplasts because they're fun and quite nice to us. They're also the home of photosynthesis -- the most interesting part of the cell, and are able to churn out huge amounts of proteins.

A Toolbox for Chloroplast Transformation

Our toolbox aims to:

  • Produce a standard method for the chloroplast transformation
  • Attempt to use self-expressing cas9 in the chloroplast, in order to achieve ploidy.
  • Provide a facility in which to grow Chalamydomonas
  • Provide an affordable biolistics design for smaller labs