Difference between revisions of "Team:Valencia UPV/Notebook"
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| − | <section><div class="container"><div class="timeline"><div class="timeline-hline"></div><div class="blog-post-item"><div class="timeline-entry rounded">18<span>May</span><div class="timeline-vline"></div></div><ul class="blog-post-info list-inline"></ul><p>Take glycerinated cultures of C58 <i>Agrobacterium</i> with dsRED from GoldenBraid Collection. Prepare broth culture (5 mL LB + 5 μL kanamycin + 5 μL Rifampin) 1:1000 and incubate overnight at 28^C. <br></p></div><div class="blog-post-item"><div class="timeline-entry rounded">19<span>May</span><div class="timeline-vline"></div></div><ul class="blog-post-info list-inline"></ul><p>Refresh previously made culture by inoculating 10 μL in a new culture medium.<br></p></div><div class="blog-post-item"><div class="timeline-entry rounded">20<span>May</span><div class="timeline-vline"></div></div><ul class="blog-post-info list-inline"></ul><p>Agroinfiltration in <i>Nicotiana benthamiana</i> of C58 with dsRED. href<br></p></div><div class="blog-post-item"><div class="timeline-entry rounded">06<span>Jun</span><div class="timeline-vline"></div></div><ul class="blog-post-info list-inline"></ul><p></p><ul><li>Take from the glycerinates of Goldenbraid Collection:</li></ul><p></p><div class="table-responsive" style="width:55%;overflow:inherit"><table class="table table-bordered table-striped"><tr><th>Plasmid</th><th>GB Code</th></tr><tr><td>pD6B3 α1</td><td>GB0015</td></tr><tr><td>pD6B3 α2</td><td>GB0017</td></tr><tr><td>pD6B3 Ω1</td><td>GB0019</td></tr><tr><td>pD6B3 Ω2</td><td>GB0021</td></tr><tr><td>pUPD2</td><td>GB0307</td></tr></table></div><p>Prepare liquid culture (3 mL LB + 3 μL antibiotic) 1:1000. Incubate at 37^C overnight. <br></p><ul><li>Experiment with snails:</li></p><ul><li>Two experiments: one with infiltrated <i>Nicotiana benthamiana</i> and another with not infiltrated N.benthamiana (negative control). Lettuce leafs haven’t been correctly infiltrated and it seems that the expression level is low.</li><li> Let both <i>N. benthamiana</i> leafs with snails overnight at room temperature in separated boxes. We will observe if snails eat the leafs and if appears fluorescence. </li></ul></ul><p><br></p></div></div></div></section> | + | <section><div class="container"><div class="timeline"><div class="timeline-hline"></div><div class="blog-post-item"><div class="timeline-entry rounded">18<span>May</span><div class="timeline-vline"></div></div><ul class="blog-post-info list-inline"></ul><p>Take glycerinated cultures of C58 <i>Agrobacterium</i> with dsRED from GoldenBraid Collection. Prepare broth culture (5 mL LB + 5 μL kanamycin + 5 μL Rifampin) 1:1000 and incubate overnight at 28^C. <br><br></p></div><div class="blog-post-item"><div class="timeline-entry rounded">19<span>May</span><div class="timeline-vline"></div></div><ul class="blog-post-info list-inline"></ul><p>Refresh previously made culture by inoculating 10 μL in a new culture medium.<br><br></p></div><div class="blog-post-item"><div class="timeline-entry rounded">20<span>May</span><div class="timeline-vline"></div></div><ul class="blog-post-info list-inline"></ul><p>Agroinfiltration in <i>Nicotiana benthamiana</i> of C58 with dsRED. href<br><br></p></div><div class="blog-post-item"><div class="timeline-entry rounded">06<span>Jun</span><div class="timeline-vline"></div></div><ul class="blog-post-info list-inline"></ul><p></p><ul><li>Take from the glycerinates of Goldenbraid Collection:</li></ul><p></p><div class="table-responsive" style="width:55%;overflow:inherit"><table class="table table-bordered table-striped"><tr><th>Plasmid</th><th>GB Code</th></tr><tr><td>pD6B3 α1</td><td>GB0015</td></tr><tr><td>pD6B3 α2</td><td>GB0017</td></tr><tr><td>pD6B3 Ω1</td><td>GB0019</td></tr><tr><td>pD6B3 Ω2</td><td>GB0021</td></tr><tr><td>pUPD2</td><td>GB0307</td></tr></table></div><p>Prepare liquid culture (3 mL LB + 3 μL antibiotic) 1:1000. Incubate at 37^C overnight. <br></p><ul><li>Experiment with snails:</li></p><ul><li>Two experiments: one with infiltrated <i>Nicotiana benthamiana</i> and another with not infiltrated N.benthamiana (negative control). Lettuce leafs haven’t been correctly infiltrated and it seems that the expression level is low.</li><li> Let both <i>N. benthamiana</i> leafs with snails overnight at room temperature in separated boxes. We will observe if snails eat the leafs and if appears fluorescence. </li></ul></ul><p><br></p></div></div></div></section> |
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Revision as of 18:30, 1 October 2016
18May
Take glycerinated cultures of C58 Agrobacterium with dsRED from GoldenBraid Collection. Prepare broth culture (5 mL LB + 5 μL kanamycin + 5 μL Rifampin) 1:1000 and incubate overnight at 28^C.
19May
Refresh previously made culture by inoculating 10 μL in a new culture medium.
20May
Agroinfiltration in Nicotiana benthamiana of C58 with dsRED. href
06Jun
- Take from the glycerinates of Goldenbraid Collection:
| Plasmid | GB Code |
|---|---|
| pD6B3 α1 | GB0015 |
| pD6B3 α2 | GB0017 |
| pD6B3 Ω1 | GB0019 |
| pD6B3 Ω2 | GB0021 |
| pUPD2 | GB0307 |
Prepare liquid culture (3 mL LB + 3 μL antibiotic) 1:1000. Incubate at 37^C overnight.
- Experiment with snails:
- Two experiments: one with infiltrated Nicotiana benthamiana and another with not infiltrated N.benthamiana (negative control). Lettuce leafs haven’t been correctly infiltrated and it seems that the expression level is low.
- Let both N. benthamiana leafs with snails overnight at room temperature in separated boxes. We will observe if snails eat the leafs and if appears fluorescence.
