Difference between revisions of "Team:CSU Fort Collins/NoteBook/JanThroughApr"
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<div class = 'Days'><h5>1/13/16:</h5></div> | <div class = 'Days'><h5>1/13/16:</h5></div> | ||
| − | <div class = 'Info'>< | + | <div class = 'Info'><ul><li>Colony PCR performed on slr0435+pSB1C3 transformed colonies</li> |
| − | < | + | <li>Ran PCR results on 1% gel</li> |
<img src = "https://static.igem.org/mediawiki/2016/c/c0/CSU_gJan16Slr0435.png"> | <img src = "https://static.igem.org/mediawiki/2016/c/c0/CSU_gJan16Slr0435.png"> | ||
| + | </ul> | ||
</div> | </div> | ||
<div class = 'Days'><h5>2/6/16:</h5></div> | <div class = 'Days'><h5>2/6/16:</h5></div> | ||
| − | <div class = 'Info'>< | + | <div class = 'Info'><ul><li>PCRed out BBA_J23106 out of glycerol stock 19 using Q5 master mix 19rxns</li> |
| + | </ul> | ||
</div> | </div> | ||
<div class = 'Days'><h5>2/9/16:</h5></div> | <div class = 'Days'><h5>2/9/16:</h5></div> | ||
| − | <div class = 'Info'>< | + | <div class = 'Info'><ul><li>Transformed Bba_K325909 from parts kit into DH5a cels</li> |
| + | </ul> | ||
</div> | </div> | ||
<div class = 'Days'><h5>2/11/16:</h5></div> | <div class = 'Days'><h5>2/11/16:</h5></div> | ||
| − | <div class = 'Info'>< | + | <div class = 'Info'><ul><li>To confirm that Bba_K325909 are functioning we tried to induce expression using L-arabinose</li> |
| − | < | + | <li>No luminescence was produced</li> |
| + | </ul> | ||
</div> | </div> | ||
<div class = 'Days'><h5>3/24/16:</h5></div> | <div class = 'Days'><h5>3/24/16:</h5></div> | ||
| − | <div class = 'Info'>< | + | <div class = 'Info'><ul><li>Set up O/N cultures of K1033929, K592009 and E1010 in 5 mL of LB with 5 uL of Cm34 antibiotic </li> |
| + | </ul> | ||
</div> | </div> | ||
<div class = 'Days'><h5>3/29/16:</h5></div> | <div class = 'Days'><h5>3/29/16:</h5></div> | ||
| − | <div class = 'Info'>< | + | <div class = 'Info'><ul><li>Ran promoters Sll027, and LrtA in a 1% gel</li> |
<h6>Sll0027</h6> | <h6>Sll0027</h6> | ||
<img src = "https://static.igem.org/mediawiki/2016/0/0f/CSU_gSll02632416.png"> | <img src = "https://static.igem.org/mediawiki/2016/0/0f/CSU_gSll02632416.png"> | ||
<h6>LrtA</h6> | <h6>LrtA</h6> | ||
<img src = "https://static.igem.org/mediawiki/2016/3/33/CSU_gLrta32416.png"> | <img src = "https://static.igem.org/mediawiki/2016/3/33/CSU_gLrta32416.png"> | ||
| + | </ul> | ||
</div> | </div> | ||
<div class = 'Days'><h5>4/6/16:</h5></div> | <div class = 'Days'><h5>4/6/16:</h5></div> | ||
| − | <div class = 'Info'>< | + | <div class = 'Info'><ul><li>Ran Cpcg2 on 1% gel</li> |
<img src = "https://static.igem.org/mediawiki/2016/f/f1/CSU_Cpcg224616.png"> | <img src = "https://static.igem.org/mediawiki/2016/f/f1/CSU_Cpcg224616.png"> | ||
| + | </ul> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 22:09, 16 October 2016
CSU iGEM
January Through April
1/4/16:
- Ligated b0034 and c0061
- Transformed Ligated mixture
- Ran PCR results on a 2% gel
1/5/16:
- Digested LuxR with EcoRI and SpeI
- Performed Colony PCR on transformation from the 4th
1/11/16:
- slr0435 Excision from Synechocystis sp. PCC6803 with PCR using Phusion(size 276 bp).
- Ran PCR results on a 2% gel
- Digested I0462 and E0030 with EcoRI and SpeI, R0062 and B0015 with EcoRI and XbaI, B0034 with SPeI and PstI, and C0061 with XbaI and PstI.
1/12/16:
- Digested slr0435 and resuspended pSB1C3 with EcoRI and PstI
- Ligated pSB1C3 and slr0435
- Transformed ligation mixture
1/13/16:
- Colony PCR performed on slr0435+pSB1C3 transformed colonies
- Ran PCR results on 1% gel
2/6/16:
- PCRed out BBA_J23106 out of glycerol stock 19 using Q5 master mix 19rxns
2/9/16:
- Transformed Bba_K325909 from parts kit into DH5a cels
2/11/16:
- To confirm that Bba_K325909 are functioning we tried to induce expression using L-arabinose
- No luminescence was produced
3/24/16:
- Set up O/N cultures of K1033929, K592009 and E1010 in 5 mL of LB with 5 uL of Cm34 antibiotic
3/29/16:
- Ran promoters Sll027, and LrtA in a 1% gel
Sll0027
LrtA
4/6/16:
- Ran Cpcg2 on 1% gel
