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<div class = 'Days'><h5>7/6/16:</h5></div> | <div class = 'Days'><h5>7/6/16:</h5></div> | ||
<div class = 'Info'><ul><li>PCRed out Lrta from <i>Synechocystis PCC 6003</i></li> | <div class = 'Info'><ul><li>PCRed out Lrta from <i>Synechocystis PCC 6003</i></li> | ||
− | |||
− | |||
</ul> | </ul> | ||
</div> | </div> | ||
Line 38: | Line 36: | ||
<div class = 'Info'><ul><li>Ran Lrta PCR products on a 1% gel</i></li> | <div class = 'Info'><ul><li>Ran Lrta PCR products on a 1% gel</i></li> | ||
<img src ="https://static.igem.org/mediawiki/2016/1/1b/CSU_Lrta7716.png"> | <img src ="https://static.igem.org/mediawiki/2016/1/1b/CSU_Lrta7716.png"> | ||
+ | </ul> | ||
+ | </div> | ||
+ | |||
+ | <div class = 'Days'><h5>7/8/16:</h5></div> | ||
+ | <div class = 'Info'><ul><li>Performed ligase cycling reaction on slr0168 and pSB1C3</li> | ||
+ | <li>Ran all PCR products on 1%gel</li> | ||
+ | <img src ="https://static.igem.org/mediawiki/2016/f/fe/CSU_SLR01687716.png"> | ||
+ | <li>Digested Lrta(SpeI EcorI)</li> | ||
+ | <li>Ligated Lrta and EYFP-LVA</li> | ||
+ | <li>Transformed Lrta+EYFP-LVA ligation mixture</li> | ||
</ul> | </ul> | ||
</div> | </div> |
Revision as of 06:29, 17 October 2016
July
7/6/16:
- PCRed out Lrta from Synechocystis PCC 6003
7/7/16:
- Ran Lrta PCR products on a 1% gel
7/8/16:
- Performed ligase cycling reaction on slr0168 and pSB1C3
- Ran all PCR products on 1%gel
- Digested Lrta(SpeI EcorI)
- Ligated Lrta and EYFP-LVA
- Transformed Lrta+EYFP-LVA ligation mixture