Difference between revisions of "Team:CSU Fort Collins/NoteBook/July"

Line 30: Line 30:
 
<div class = 'Days'><h5>7/6/16:</h5></div>
 
<div class = 'Days'><h5>7/6/16:</h5></div>
 
<div class = 'Info'><ul><li>PCRed out Lrta from <i>Synechocystis PCC 6003</i></li>
 
<div class = 'Info'><ul><li>PCRed out Lrta from <i>Synechocystis PCC 6003</i></li>
 
<img src ="https://static.igem.org/mediawiki/2016/2/2a/CSU_Slr01685116.png">
 
 
</ul>
 
</ul>
 
</div>
 
</div>
Line 38: Line 36:
 
<div class = 'Info'><ul><li>Ran Lrta PCR products on a 1% gel</i></li>
 
<div class = 'Info'><ul><li>Ran Lrta PCR products on a 1% gel</i></li>
 
<img src ="https://static.igem.org/mediawiki/2016/1/1b/CSU_Lrta7716.png">
 
<img src ="https://static.igem.org/mediawiki/2016/1/1b/CSU_Lrta7716.png">
 +
</ul>
 +
</div>
 +
 +
<div class = 'Days'><h5>7/8/16:</h5></div>
 +
<div class = 'Info'><ul><li>Performed ligase cycling reaction on slr0168 and pSB1C3</li>
 +
<li>Ran all PCR products on 1%gel</li>
 +
<img src ="https://static.igem.org/mediawiki/2016/f/fe/CSU_SLR01687716.png">
 +
<li>Digested Lrta(SpeI EcorI)</li>
 +
<li>Ligated Lrta and EYFP-LVA</li>
 +
<li>Transformed Lrta+EYFP-LVA ligation mixture</li>
 
</ul>
 
</ul>
 
</div>
 
</div>

Revision as of 06:29, 17 October 2016

July

7/6/16:
  • PCRed out Lrta from Synechocystis PCC 6003
7/7/16:
  • Ran Lrta PCR products on a 1% gel
7/8/16:
  • Performed ligase cycling reaction on slr0168 and pSB1C3
  • Ran all PCR products on 1%gel
  • Digested Lrta(SpeI EcorI)
  • Ligated Lrta and EYFP-LVA
  • Transformed Lrta+EYFP-LVA ligation mixture