Difference between revisions of "Team:CSU Fort Collins/NoteBook/July"

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<div class = 'Days'><h5>7/12/16:</h5></div>
 
<div class = 'Days'><h5>7/12/16:</h5></div>
−
<div class = 'Info'><ul>Dpn1 Digested slr0168  and pSB1C3</li>
+
<div class = 'Info'><ul><li>Dpn1 Digested slr0168  and pSB1C3</li>
 
<li>Phosphorylated slr0168 and pSB1C3</li>
 
<li>Phosphorylated slr0168 and pSB1C3</li>
 
<li>Performed ligase cycling reaction on phosphorylated mixture</li>
 
<li>Performed ligase cycling reaction on phosphorylated mixture</li>

Revision as of 06:39, 17 October 2016

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CSU iGEM



July

7/6/16:
  • PCRed out Lrta from Synechocystis PCC 6003
7/7/16:
  • Ran Lrta PCR products on a 1% gel
7/8/16:
  • Performed ligase cycling reaction on slr0168 and pSB1C3
  • Ran all PCR products on 1%gel
  • Digested Lrta(SpeI EcorI)
  • Ligated Lrta and EYFP-LVA
  • Transformed Lrta+EYFP-LVA ligation mixture
7/12/16:
  • Dpn1 Digested slr0168 and pSB1C3
  • Phosphorylated slr0168 and pSB1C3
  • Performed ligase cycling reaction on phosphorylated mixture
  • Transformed ligase cycling reaction products
  • Digested EYFP-LVA(PstI)
  • Ligated EYFP-LVA + LrtA
  • Transformed EYFP-LVA+LrtA ligation mixture