Difference between revisions of "Team:CSU Fort Collins/NoteBook/July"
| Line 57: | Line 57: | ||
<li>Ligated EYFP-LVA + LrtA</li> | <li>Ligated EYFP-LVA + LrtA</li> | ||
<li>Transformed EYFP-LVA+LrtA ligation mixture</li> | <li>Transformed EYFP-LVA+LrtA ligation mixture</li> | ||
| + | <li>Performed colony PCR on 13 colonies with the Q1 block</li> | ||
| + | <li>Ran PCR results on a 1.5% gel</li> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/e/e8/CSU_Q171216.png"> | ||
</ul> | </ul> | ||
</div> | </div> | ||
Revision as of 14:38, 17 October 2016
CSU iGEM
July
7/6/16:
- PCRed out Lrta from Synechocystis PCC 6003
7/7/16:
- Ran Lrta PCR products on a 1% gel
7/8/16:
- Performed ligase cycling reaction on slr0168 and pSB1C3
- Ran all PCR products on 1%gel
- Digested Lrta(SpeI EcorI)
- Ligated Lrta and EYFP-LVA
- Transformed Lrta+EYFP-LVA ligation mixture
7/12/16:
- Dpn1 Digested slr0168 and pSB1C3
- Phosphorylated slr0168 and pSB1C3
- Performed ligase cycling reaction on phosphorylated mixture
- Transformed ligase cycling reaction products
- Digested EYFP-LVA(PstI)
- Ligated EYFP-LVA + LrtA
- Transformed EYFP-LVA+LrtA ligation mixture
- Performed colony PCR on 13 colonies with the Q1 block
- Ran PCR results on a 1.5% gel
7/13/16:
- Colony PCR performed on 10 EYFP-LVA+LrtA transformed colonies
