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Revision as of 15:48, 17 October 2016
CSU iGEM
August
8/6/16:
- Performed Colony PCR on Slr0118+Luc transformed colonies
- Ran PCR results on a 1% gel
- Gel had no bands
8/8/16:
- Digested I712013 and Luc
- Ligated I712013 and Luc
- Transformed ligation mixture
8/11/16:
- Colony PCRed transformed 21 I7+Luc colonies
8/12/16:
- Phosphorylated Q1 and Q2 for ligase cycling
8/15/16:
- Performed ligase cycling reaction on phosphorylated Q block mixture
8/19/16:
- Performed colony PCR on Slr0168+Luc
