Difference between revisions of "Team:NYU-AD/Results"
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| − | <p> | + | <p>Throughout our project, we have successfully transformed E. coli competent cells and validated the expression of the Gb3 synthase mRNA from the arabinose inducible part using RT-PCR and PCR amplification. Figure 1 shows colony growth of appropriate selective media[JK1] . |
| + | Bands corresponding to amplified Gb3 synthase products resulting from the three different sets of primers [JK2] used are shown in Figure 2[JK3] . </p> | ||
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| + | <p>Additionally, we have validated the production of Indole in E. coli cells transformed with Part:BBa_K1867024 using the Kovac’s reagent tube test. Figure3[JK4] represents the change in color as an indication of indole production from transformed cells.</p> | ||
| − | + | <p>We have successfully shown a band migration shift in a “gel-shift” experiment displaying a difference in migration patterns of Subunit B-Gb3 complex in comparison with a pure Subunit B sample and more details are shown in our proof of concept page.</p> | |
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| − | + | <p>Finally, we have successfully designed and 3D printed a prototype of our device that will eventually, once fully functional, will allow for the detection Shiga toxin contaminations of food samples in a period of time not exceeding 30 mins with no lab setup required and in a user friendly, mostly hands-off manner. Figures 4 and 5 display our design and show the prototyped device in 3D[JK5] .</p> | |
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| − | + | <p>All of our results are reproducible using the protocols we followed, which are available in our experiments page.</p> | |
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Revision as of 16:30, 19 October 2016
Throughout our project, we have successfully transformed E. coli competent cells and validated the expression of the Gb3 synthase mRNA from the arabinose inducible part using RT-PCR and PCR amplification. Figure 1 shows colony growth of appropriate selective media[JK1] . Bands corresponding to amplified Gb3 synthase products resulting from the three different sets of primers [JK2] used are shown in Figure 2[JK3] .
Additionally, we have validated the production of Indole in E. coli cells transformed with Part:BBa_K1867024 using the Kovac’s reagent tube test. Figure3[JK4] represents the change in color as an indication of indole production from transformed cells.
We have successfully shown a band migration shift in a “gel-shift” experiment displaying a difference in migration patterns of Subunit B-Gb3 complex in comparison with a pure Subunit B sample and more details are shown in our proof of concept page.
Finally, we have successfully designed and 3D printed a prototype of our device that will eventually, once fully functional, will allow for the detection Shiga toxin contaminations of food samples in a period of time not exceeding 30 mins with no lab setup required and in a user friendly, mostly hands-off manner. Figures 4 and 5 display our design and show the prototyped device in 3D[JK5] .
All of our results are reproducible using the protocols we followed, which are available in our experiments page.
