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<span class="header"><a id="Galaxy1_Hamburger1_Burgers_Link_0" href="https://2016.igem.org/Team:Tsinghua">Home</a></span> | <span class="header"><a id="Galaxy1_Hamburger1_Burgers_Link_0" href="https://2016.igem.org/Team:Tsinghua">Home</a></span> | ||
<ul class="subs"> | <ul class="subs"> | ||
− | <img src="https://static.igem.org/mediawiki/2016/d/d7/T--Tsinghua--IGEM_white_letters.png" style="width: | + | <img src="https://static.igem.org/mediawiki/2016/d/d7/T--Tsinghua--IGEM_white_letters.png" style="width:178px;height:150px;"> |
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<li><a id="Galaxy1_Hamburger1_Burgers_Children_1_Link_0" href="https://2016.igem.org/Team:Tsinghua/Team">Team members</a></li> | <li><a id="Galaxy1_Hamburger1_Burgers_Children_1_Link_0" href="https://2016.igem.org/Team:Tsinghua/Team">Team members</a></li> | ||
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_1_Link_1" href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_1_Link_1" href="https://2016.igem.org/Team:Tsinghua/Attributions">Attributions</a></li> |
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_1_Link_2" href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_1_Link_2" href="https://2016.igem.org/Team:Tsinghua/Acknowledgement">Acknowledgement</a></li> |
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_1_Link_3" href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_1_Link_3" href="https://2016.igem.org/Team:Tsinghua/Acknowledgement#tag_sponsorship">Sponsorship</a></li> |
+ | <br> | ||
<br> | <br> | ||
<br> | <br> | ||
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</li> | </li> | ||
<li class="lvl1"> | <li class="lvl1"> | ||
− | <span class="header"><a id="Galaxy1_Hamburger1_Burgers_Link_2" href="https://2016.igem.org/Team:Tsinghua/ | + | <span class="header"><a id="Galaxy1_Hamburger1_Burgers_Link_2" href="https://2016.igem.org/Team:Tsinghua/Description">Project</a></span> |
<ul class="subs"> | <ul class="subs"> | ||
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_2_Link_0" href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_2_Link_0" href="https://2016.igem.org/Team:Tsinghua/Description">Description</a></li> |
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_2_Link_1" href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_2_Link_1" href="https://2016.igem.org/Team:Tsinghua/Experiments">Design</a></li> |
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_2_Link_2" href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_2_Link_2" href="https://2016.igem.org/Team:Tsinghua/Results">Results</a></li> |
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_2_Link_3" href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_2_Link_3" href="https://2016.igem.org/Team:Tsinghua/Proof">Proof of Concepts</a></li> |
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_2_Link_4" href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_2_Link_4" href="https://2016.igem.org/Team:Tsinghua/Notebook">Notebook</a></li> |
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_2_Link_5" href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_2_Link_5" href="https://2016.igem.org/Team:Tsinghua/Model">Modeling</a></li> |
+ | <li><a id="Galaxy1_Hamburger1_Burgers_Children_2_Link_5" href="https://2016.igem.org/Team:Tsinghua/Safety">Safety</a></li> | ||
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<span class="header"><a id="Galaxy1_Hamburger1_Burgers_Link_3" href="https://2016.igem.org/Team:Tsinghua/Parts">Parts</a></span> | <span class="header"><a id="Galaxy1_Hamburger1_Burgers_Link_3" href="https://2016.igem.org/Team:Tsinghua/Parts">Parts</a></span> | ||
<ul class="subs"> | <ul class="subs"> | ||
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_3_Link_0" href="https://2016.igem.org/Team:Tsinghua/Parts | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_3_Link_0" href="https://2016.igem.org/Team:Tsinghua/Parts#tag_summary">Parts Summary</a></li> |
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_3_Link_1" href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_3_Link_1" href="https://2016.igem.org/Team:Tsinghua/Basic_Part#tag_basic">Basic Parts</a></li> |
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_3_Link_2" href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_3_Link_2" href="https://2016.igem.org/Team:Tsinghua/Composite_Part">Composite Parts</a></li> |
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</ul> | </ul> | ||
</li> | </li> | ||
<li class="lvl1"> | <li class="lvl1"> | ||
− | <span class="header"><a id="Galaxy1_Hamburger1_Burgers_Link_3" href="https://2016.igem.org/Team:Tsinghua/ | + | <span class="header"><a id="Galaxy1_Hamburger1_Burgers_Link_3" href="https://2016.igem.org/Team:Tsinghua/Human_Practices">Human Practices</a></span> |
<ul class="subs"> | <ul class="subs"> | ||
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_3_Link_0" href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_3_Link_0" href="https://2016.igem.org/Team:Tsinghua/Human_Practices">Overview</a></li> |
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_3_Link_1" href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_3_Link_1" href="https://2016.igem.org/Team:Tsinghua/Human_Practices#tag_engagement">Public Engagement</a></li> |
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_3_Link_2" href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_3_Link_0" href="https://2016.igem.org/Team:Tsinghua/Collaborations">Collaboration</a></li> |
+ | <li><a id="Galaxy1_Hamburger1_Burgers_Children_3_Link_1" href="https://2016.igem.org/Team:Tsinghua/Community_meetup">Community Meetup</a></li> | ||
+ | <li><a id="Galaxy1_Hamburger1_Burgers_Children_3_Link_2" href="https://2016.igem.org/Team:Tsinghua/Integrated_Practices">Integrated Practices</a></li> | ||
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<span class="header"><a id="Galaxy1_Hamburger1_Burgers_Link_4" href="https://2016.igem.org/Team:Tsinghua/MedalChecklist">Medal Checklist</a></span> | <span class="header"><a id="Galaxy1_Hamburger1_Burgers_Link_4" href="https://2016.igem.org/Team:Tsinghua/MedalChecklist">Medal Checklist</a></span> | ||
<ul class="subs"> | <ul class="subs"> | ||
− | <li><a id="Galaxy1_Hamburger1_Burgers_Children_4_Link_0" href="https://2016.igem.org/Team:Tsinghua/MedalChecklist#bronze | + | <li><a id="Galaxy1_Hamburger1_Burgers_Children_4_Link_0" href="https://2016.igem.org/Team:Tsinghua/MedalChecklist#bronze">Bronze</a></li> |
<li><a id="Galaxy1_Hamburger1_Burgers_Children_4_Link_1" href="https://2016.igem.org/Team:Tsinghua/MedalChecklist#silver">Silver</a></li> | <li><a id="Galaxy1_Hamburger1_Burgers_Children_4_Link_1" href="https://2016.igem.org/Team:Tsinghua/MedalChecklist#silver">Silver</a></li> | ||
<li><a id="Galaxy1_Hamburger1_Burgers_Children_4_Link_2" href="https://2016.igem.org/Team:Tsinghua/MedalChecklist#gold">Gold</a></li> | <li><a id="Galaxy1_Hamburger1_Burgers_Children_4_Link_2" href="https://2016.igem.org/Team:Tsinghua/MedalChecklist#gold">Gold</a></li> | ||
+ | <br> | ||
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<li><a href="https://2016.igem.org/Team:Tsinghua">Home</a></li> | <li><a href="https://2016.igem.org/Team:Tsinghua">Home</a></li> | ||
<li><a href="https://2016.igem.org/Team:Tsinghua/Team">Team</a></li> | <li><a href="https://2016.igem.org/Team:Tsinghua/Team">Team</a></li> | ||
− | <li><a href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a href="https://2016.igem.org/Team:Tsinghua/Description">Project</a></li> |
<li><a href="https://2016.igem.org/Team:Tsinghua/Parts">Parts</a></li> | <li><a href="https://2016.igem.org/Team:Tsinghua/Parts">Parts</a></li> | ||
− | <li><a href="https://2016.igem.org/Team:Tsinghua/ | + | <li><a href="https://2016.igem.org/Team:Tsinghua/Human_Practices">Human practice</a></li> |
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</nav> | </nav> | ||
</div> | </div> | ||
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− | + | <a href="https://2016.igem.org/Team:Tsinghua" style="margin-right:-30px"> | |
− | + | <img alt="" src="https://static.igem.org/mediawiki/2016/5/50/T--Tsinghua--navigatorImg.png" style="width:40%;height:40%;margin-left:280px;margin-top:-12px"/> | |
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<br> limited to excessive cell death, abnormal proliferation, untimely cell senescence, etc. Therefore, a gene | <br> limited to excessive cell death, abnormal proliferation, untimely cell senescence, etc. Therefore, a gene | ||
<br> mutation surveillance system that can sensitively and efficiently detect and remove such aberrations | <br> mutation surveillance system that can sensitively and efficiently detect and remove such aberrations | ||
− | <br> <i>in vivo</i> is required. | + | <br> <i>in vivo</i>is required. |
+ | <br> | ||
<br> | <br> | ||
<br><i>Saccharomyces cerevisiae</i> is chosen as the model organism for designing such a gene surveillance system. | <br><i>Saccharomyces cerevisiae</i> is chosen as the model organism for designing such a gene surveillance system. | ||
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<div class="contentPage" style="margin-left:200px;margin-top:20px;width:1130px;"> | <div class="contentPage" style="margin-left:200px;margin-top:20px;width:1130px;"> | ||
<h2 class="contentPage"><br>Reference<img id="referenceButton" onclick="hideRef()" src="https://static.igem.org/mediawiki/2016/0/06/T--Tsinghua--details_open.png" style="align:center;"></h2> | <h2 class="contentPage"><br>Reference<img id="referenceButton" onclick="hideRef()" src="https://static.igem.org/mediawiki/2016/0/06/T--Tsinghua--details_open.png" style="align:center;"></h2> | ||
− | + | <br> | |
<div id="reference" style="display:none;"> | <div id="reference" style="display:none;"> | ||
[1] Hanahan, D., & Weinberg, R. A. (2011). Hallmarks of cancer: the next generation. Cell, 144(5), 646-674. | [1] Hanahan, D., & Weinberg, R. A. (2011). Hallmarks of cancer: the next generation. Cell, 144(5), 646-674. | ||
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<br> in living cells. Proc. Natl. Acad. Sci. USA 95, 11538–11543. | <br> in living cells. Proc. Natl. Acad. Sci. USA 95, 11538–11543. | ||
<br>[6] Fouts, D.E., True, H.L., and Celander, D.W. (1997). Functional recognition of fragmented operator sites | <br>[6] Fouts, D.E., True, H.L., and Celander, D.W. (1997). Functional recognition of fragmented operator sites | ||
− | <br> by R17/MS2 coat protein, a translational repressor. Nucleic Acids Res. 25, 4464–4473. | + | <br> by R17/MS2 coat protein, a translational repressor. Nucleic Acids Res. 25, 4464–4473.<br> |
<br>[7] Nelles, D. A., Fang, M. Y., O’Connell, M. R., Xu, J. L., Markmiller, S. J., Doudna, J. A., & Yeo, | <br>[7] Nelles, D. A., Fang, M. Y., O’Connell, M. R., Xu, J. L., Markmiller, S. J., Doudna, J. A., & Yeo, | ||
− | <br> G. W. (2016). Programmable RNA Tracking in Live Cells with CRISPR/Cas9. Cell, 165(2), 488-496. | + | <br> G. W. (2016). Programmable RNA Tracking in Live Cells with CRISPR/Cas9. Cell, 165(2), 488-496.<br> |
<br>[8] Farzadfard, F., & Lu, T. K. (2014). Genomically encoded analog memory with precise in vivo DNA writing | <br>[8] Farzadfard, F., & Lu, T. K. (2014). Genomically encoded analog memory with precise in vivo DNA writing | ||
<br> in living cell populations. Science, 346(6211), 1256272. | <br> in living cell populations. Science, 346(6211), 1256272. | ||
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<br>[10] Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg, J. S., Kriz, A. J., ... & Koonin, E. V. | <br>[10] Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg, J. S., Kriz, A. J., ... & Koonin, E. V. | ||
<br> (2015). In vivo genome editing using Staphylococcus aureus Cas9. Nature, 520(7546), 186-191. | <br> (2015). In vivo genome editing using Staphylococcus aureus Cas9. Nature, 520(7546), 186-191. | ||
− | <br>[11] Sherman, F. (1991). [1] Getting started with yeast. Methods in enzymology, 194, 3-21. | + | <br>[11] Sherman, F. (1991). [1] Getting started with yeast. Methods in enzymology, 194, 3-21.<br> |
<br>[12] O’Connell, M.R., Oakes, B.L., Sternberg, S.H., East-Seletsky, A., Kaplan, M., and Doudna, J.A. (2014). | <br>[12] O’Connell, M.R., Oakes, B.L., Sternberg, S.H., East-Seletsky, A., Kaplan, M., and Doudna, J.A. (2014). | ||
− | <br> Programmable RNA recognition and cleavage by CRISPR/Cas9. Nature 516, 263–266. | + | <br> Programmable RNA recognition and cleavage by CRISPR/Cas9. Nature 516, 263–266. <br> |
<br>[13] Vidal, M., Brachmann, R. K., Fattaey, A., Harlow, E., & Boeke, J. D. (1996). Reverse two-hybrid and | <br>[13] Vidal, M., Brachmann, R. K., Fattaey, A., Harlow, E., & Boeke, J. D. (1996). Reverse two-hybrid and | ||
<br> one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions. Proceedings | <br> one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions. Proceedings | ||
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Revision as of 21:37, 19 October 2016