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Take from the glycerinates of Goldenbraid Collection:
Plasmid GB Code pD6B3 α1 GB0015 pD6B3 α2 GB0017 pD6B3 Ω1 GB0019 pD6B3 Ω2 GB0021 pUPD2 GB0307 -
Prepare liquid culture (3 mL LB + 3 μL antibiotic) 1:1000. Incubate at 37°C overnight.
- Experiment with snails:
-
Let both N. benthamianaleafs with snails overnight at room temperature in separated boxes. We will observe if snails eat the leafs and if appears fluorescence.
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Revision as of 14:20, 1 August 2016
May 18, 2016
Take glycerinated cultures of C58 Agrobacterium with dsRED from GoldenBraid Collection. Prepare broth culture (5 mL LB + 5 μL kanamycin + 5 μL Rifampin) 1:1000 and incubate overnight at 28°C.
May 19, 2016
Refresh previously made culture by inoculating 10 μL in a new culture medium.
