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Revision as of 21:26, 16 September 2016
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Background
blah blah blah blahWhy use Chlamydomonas Reinhardtii?
We intend to use Chlamydomonas Reinhardtii as a simple model to represent higher order systems.
There are many reasons why one would want to chose Chlamydomonas Reinhardtii:
- Chlamydomonas reinhardtii is a unicellular alga which is a relatively well established model organism and many techniques can be readily transferred to higher plants.
- It is registered as safe to humans so there is a lot of interest in developing edible vaccines and other foodstuffs.
- Fast growing
- Very efficient homologous recombination in chloroplast
- Conveniently only has one (quite large) chloroplast
Why Chloroplasts?
We chose chloroplasts because they're fun and quite nice to us. They're also the home of photosynthesis -- the most interesting part of the cell, and are able to churn out huge amounts of proteins.
A Toolbox for Chloroplast Transformation
Our toolbox aims to:
- Produce a standard method for the chloroplast transformation
- Attempt to use self-expressing cas9 in the chloroplast, in order to achieve ploidy.
- Provide a facility in which to grow Chalamydomonas
- Provide an affordable biolistics design for smaller labs