Difference between revisions of "Team:Edinburgh UG/Demonstrate"
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<h1>Demonstrate</h1> | <h1>Demonstrate</h1> | ||
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<div class="featurette" id="about"> | <div class="featurette" id="about"> | ||
| − | + | <p class="lead"> Every iGEM project contributes towards interest and advancement in synthetic biology. However the transition from an interesting lab project to a commercial product is a big step. We believe that DNA will become a long-term storage medium, and so it is important to look to the future of this being implemented, and show how our system can be used in the real world. </p> | |
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<img class="featurette-image img-circle img-responsive pull-left" src="http://placehold.it/500x500"> | <img class="featurette-image img-circle img-responsive pull-left" src="http://placehold.it/500x500"> | ||
| − | <h2 class="featurette-heading"> | + | <h2 class="featurette-heading">Use of Biological Materials |
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</h2> | </h2> | ||
| − | <p class="lead"> | + | <p class="lead">Any team considering the release of a biological product must be fully aware of rules and regulations governing their country. In Scotland, EU regulation is implemented, along with further additions by both the UK and Scottish Government. </p> |
| + | <p class="lead">Our BabbleBlock generation process can be divided into three parts; the generation of BabbleBricks housed in acceptor plasmids, assembly of BabbleBlocks, and long-term storage of BabbleBlocks. The generation of BabbleBricks involves transformation of competent TOP10 E.coli cells. The assembly and storage of BabbleBricks and Blocks however is cell free. </p> | ||
| + | <p class="lead">The distinction between these two processes is important. The use of unnatural DNA sequences outside the lab is heavily governed. Most genetically modified organism laws however cover GMOs (genetically modified organisms) or (Genetically modified micro-organisms). </p> | ||
| + | <p class="lead">“Micro-organism: This term covers bacteria, fungi and viruses, as well as cell and tissue cultures from plants, animals or humans. Naked nucleic acid, oligonucleotides, synthetic DNA, plasmids or liposomes are not considered to be micro-organisms.” - The Genetically Modified Organisms (Contained Use) Regulations 2014. Scottish Government </p> | ||
| + | <p class="lead">In Scotland, the definition of a microorganism does not cover naked DNA. All relevant governing GM legislation in Scotland refers to GMOs and GMMs, and so exclusion of naked DNA from this allows storage of assembled BabbleBlocks outside the lab. </p> | ||
| + | <p class="lead"> Our functional proof of concept follows the steps our commercialised system would have:</p> | ||
| + | <p class="lead">1. The company will make BabbleBricks </p> | ||
| + | <p class="lead">2. The company will assemble Babble Blocks </p> | ||
| + | <p class="lead">3. The user will store BabbleBricks and BabbleBlocks for use whenever needed. </p> | ||
| + | <p class="lead">4. The user will read back the data stored when needed. </p> | ||
</div> | </div> | ||
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<div class="featurette" id="contact"> | <div class="featurette" id="contact"> | ||
<img class="featurette-image img-circle img-responsive pull-right" src="http://placehold.it/500x500"> | <img class="featurette-image img-circle img-responsive pull-right" src="http://placehold.it/500x500"> | ||
| − | <h2 class="featurette-heading"> | + | <h2 class="featurette-heading">Make BabbleBricks |
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</h2> | </h2> | ||
| − | <p class="lead"> | + | <p class="lead">As demonstrated in Proof-of-concept (link page) we have made phytobrick compatible BabbleBricks. These plasmids are the DNA component of the BabbleBrick Library. For proof-of-concept we have made X, however our 5bp coding regions allows for 1024 different BabbleBricks to exist in the library. This can be scaled up; for example, a coding region of 6 nucleotides would give 4096 different BabbleBricks. The BabbleBrick Library (and complete BabbleBlocks) would be stored in barcoded tubes for integration with an automated system for assembly, and catalogue system for storing the completed constructs.</p> |
</div> | </div> | ||
<hr class="featurette-divider"> | <hr class="featurette-divider"> | ||
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| + | <div class="featurette" id="contact"> | ||
| + | <img class="featurette-image img-circle img-responsive pull-right" src="http://placehold.it/500x500"> | ||
| + | <h2 class="featurette-heading">Demo 3 | ||
| + | <span class="text-muted">Example 3</span> | ||
| + | </h2> | ||
| + | <p class="lead">Text 3</p> | ||
| + | </div> | ||
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| + | <hr class="featurette-divider"> | ||
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<!-- Footer --> | <!-- Footer --> | ||
Revision as of 14:36, 15 October 2016
Demonstrate
Every iGEM project contributes towards interest and advancement in synthetic biology. However the transition from an interesting lab project to a commercial product is a big step. We believe that DNA will become a long-term storage medium, and so it is important to look to the future of this being implemented, and show how our system can be used in the real world.
Use of Biological Materials
Any team considering the release of a biological product must be fully aware of rules and regulations governing their country. In Scotland, EU regulation is implemented, along with further additions by both the UK and Scottish Government.
Our BabbleBlock generation process can be divided into three parts; the generation of BabbleBricks housed in acceptor plasmids, assembly of BabbleBlocks, and long-term storage of BabbleBlocks. The generation of BabbleBricks involves transformation of competent TOP10 E.coli cells. The assembly and storage of BabbleBricks and Blocks however is cell free.
The distinction between these two processes is important. The use of unnatural DNA sequences outside the lab is heavily governed. Most genetically modified organism laws however cover GMOs (genetically modified organisms) or (Genetically modified micro-organisms).
“Micro-organism: This term covers bacteria, fungi and viruses, as well as cell and tissue cultures from plants, animals or humans. Naked nucleic acid, oligonucleotides, synthetic DNA, plasmids or liposomes are not considered to be micro-organisms.” - The Genetically Modified Organisms (Contained Use) Regulations 2014. Scottish Government
In Scotland, the definition of a microorganism does not cover naked DNA. All relevant governing GM legislation in Scotland refers to GMOs and GMMs, and so exclusion of naked DNA from this allows storage of assembled BabbleBlocks outside the lab.
Our functional proof of concept follows the steps our commercialised system would have:
1. The company will make BabbleBricks
2. The company will assemble Babble Blocks
3. The user will store BabbleBricks and BabbleBlocks for use whenever needed.
4. The user will read back the data stored when needed.
Make BabbleBricks
As demonstrated in Proof-of-concept (link page) we have made phytobrick compatible BabbleBricks. These plasmids are the DNA component of the BabbleBrick Library. For proof-of-concept we have made X, however our 5bp coding regions allows for 1024 different BabbleBricks to exist in the library. This can be scaled up; for example, a coding region of 6 nucleotides would give 4096 different BabbleBricks. The BabbleBrick Library (and complete BabbleBlocks) would be stored in barcoded tubes for integration with an automated system for assembly, and catalogue system for storing the completed constructs.
Demo 3 Example 3
Text 3
