Difference between revisions of "Team:CSU Fort Collins/NoteBook/JanThroughApr"

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<div class = 'Days'><h5>1/4/16:</h5></div>
 
<div class = 'Days'><h5>1/4/16:</h5></div>
−
<div class = 'Info'><p>Ligated b0034 and c0061 </p> <p>Transformed Ligated mixture</p>
+
<div class = 'Info'><ul><li>Ligated b0034 and c0061 </p> <p>Transformed Ligated mixture</li>
−
<p>Ran PCR results on a 2% gel</p>
+
<li>Ran PCR results on a 2% gel</li>
 +
</ul>
 
</div>
 
</div>
  
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<div class = 'Days'><h5>1/11/16:</h5></div>
 
<div class = 'Days'><h5>1/11/16:</h5></div>
−
<div class = 'Info'><p>slr0435 Excision from <i>Synechocystis sp. PCC6803</i> with PCR using Phusion(size 276 bp).</p>
+
<div class = 'Info'><ul><li>slr0435 Excision from <i>Synechocystis sp. PCC6803</i> with PCR using Phusion(size 276 bp).</li>
−
<p>Ran PCR results on a 2% gel</p>
+
<li>Ran PCR results on a 2% gel</li>
 
<img src = "https://static.igem.org/mediawiki/2016/e/ec/CSU_gJan152016.png">
 
<img src = "https://static.igem.org/mediawiki/2016/e/ec/CSU_gJan152016.png">
−
<p>Digested I0462 and  E0030 with EcoRI and  SpeI, R0062 and B0015 with EcoRI and XbaI, B0034 with SPeI and PstI, and C0061 with XbaI and PstI.</p>
+
<li>Digested I0462 and  E0030 with EcoRI and  SpeI, R0062 and B0015 with EcoRI and XbaI, B0034 with SPeI and PstI, and C0061 with XbaI and PstI.</li>
 +
</ul>
 
</div>
 
</div>
  
 
<div class = 'Days'><h5>1/12/16:</h5></div>
 
<div class = 'Days'><h5>1/12/16:</h5></div>
−
<div class = 'Info'><p>Digested slr0435 and resuspended pSB1C3 with EcoRI and PstI</p>
+
<div class = 'Info'><ul><li>Digested slr0435 and resuspended pSB1C3 with EcoRI and PstI</li>
−
<p>Ligated pSB1C3 and slr0435</p>
+
<li>Ligated pSB1C3 and slr0435</li>
−
<p>Transformed ligation mixture</p>
+
<li>Transformed ligation mixture</li>
 +
</ul>
 
</div>
 
</div>
  

Revision as of 22:05, 16 October 2016

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CSU iGEM



January Through April

1/4/16:
  • Ligated b0034 and c0061

    Transformed Ligated mixture

  • Ran PCR results on a 2% gel
1/5/16:
  • Digested LuxR with EcoRI and SpeI
  • Performed Colony PCR on transformation from the 4th
1/11/16:
  • slr0435 Excision from Synechocystis sp. PCC6803 with PCR using Phusion(size 276 bp).
  • Ran PCR results on a 2% gel
  • Digested I0462 and E0030 with EcoRI and SpeI, R0062 and B0015 with EcoRI and XbaI, B0034 with SPeI and PstI, and C0061 with XbaI and PstI.
1/12/16:
  • Digested slr0435 and resuspended pSB1C3 with EcoRI and PstI
  • Ligated pSB1C3 and slr0435
  • Transformed ligation mixture
1/13/16:

Colony PCR performed on slr0435+pSB1C3 transformed colonies

Ran PCR results on 1% gel

2/6/16:

PCRed out BBA_J23106 out of glycerol stock 19 using Q5 master mix 19rxns

2/9/16:

Transformed Bba_K325909 from parts kit into DH5a cels

2/11/16:

To confirm that Bba_K325909 are functioning we tried to induce expression using L-arabinose

No luminescence was produced

3/24/16:

Set up O/N cultures of K1033929, K592009 and E1010 in 5 mL of LB with 5 uL of Cm34 antibiotic

3/29/16:

Ran promoters Sll027, and LrtA in a 1% gel

Sll0027
LrtA
4/6/16:

Ran Cpcg2 on 1% gel