Revision as of 05:27, 17 October 2016

• BACKGROUND
• EXPERIMENTS
  Promoters miRNA Recombinase Combining parts Collaborations
• FUTURE WORK & DESIGN
  Circuit Design Clinical Application
• HUMAN PRACTICES
  Accessibility Integrated Human Practices Public Outreach
• TEAM
  Team Acknowledgements
• NOTEBOOK
  Lab Notebook Basic Parts Parts collection Attributions Safety

L7Ae - Kink turn

RNA-Based Gene Regulation

L7Ae, an archaeal ribosomal protein, binds with high affinity to RNA motifs called kink-turns (K-turns), found in both archaeal and eukaryote RNAs [1][2][3]. L7Ae protein sequence is divided into three structural regions consisting of a highly conserved RNA-binding region (RBR) flanked by less conserved N-terminal and C-terminal regions [2]. Variation in the terminal regions could dictate RNA-binding specificity of different homologs of L7Ae protein [2]. When a K-turn motif is inserted into the target mRNA upstream of the open reading frame, L7Ae can be used as a translational regulator [1][2][3]. The binding activity of L7Ae will prevent the ribosome machinery from performing translation. The strength of the repression can be controlled by varying the distance between the K-turns and the 5’-end of the mRNA, or by changing the number of the k-turn motifs [1].

Figure. Binding of L7Ae to kink-turn motifs preveting translation.

Recombinase and L7Ae-Kturn

Purpose

Experimental Setup

Result

Testing the 2x k-turn L7Ae system with varied L7Ae expression level

Testing the effect of varying k-turn sequences