Team:MIT/accessible protocols

• BACKGROUND
• EXPERIMENTS
  Synthetic Promoters microRNA Recombinase Proof of Concept Collaborations
• FUTURE WORK
  Circuit Design Clinical Applications
• HUMAN PRACTICES
  Parts Toolbox Accessible Protocols Integrated Human Practices Public Outreach Interlab Study
• NOTEBOOK
  Lab Notebook Parts Mammalian Parts Collection Attributions Safety
• AWARDS
  HP Silver HP Gold Integrated HP Basic Part
• TEAM

Synthetic Biology Protocols for Novices

Doing synthetic biology is hard! Especially if you have never done it before! Therefore, we took it upon ourselves, as a part of our human practices work, to create a lively and explanatory platform for making novices' job a little easier in their first voyages into the magical land of Syn-Bio! Our blog - which can be reached via this link- features the mainstream synthetic biology lab procedures, as broken down in stepwise fashion, with photographs. We used fold.cm, through tumblr.com, in the creation of this blog due to Fold's unique interface that enables one to easily link up sentences with images, hence serve both literary and visual cognition simultaneously.

However iGEM wiki rules doesn't allow content to be hosted outside of 2016.igem.org. Therefore below we include several screenshots in order to give a feel for how the platform works. Each image is hyperlinked with the corresponding page, so feel free to visit the platform and try out our protocols!

↑ The image above is the screenshot of the blog's main page where the all the procedures are listed with an accompanying illustration that describes the essence of that procedure. In order to see a quick explanation of the procedure, one can hover over the little magnifier symbol on the upper right corner of the illustration as shown below:

Then one can click on any of the illustrative icons to get more information about that specific procedure:

↑ The image above is the screenshot of an exemplary inner page that firstly includes a brief description of the procedure, then presents a link (highlighted in yellow) to the Fold page where tutorial resides. The screenshot of the Fold page can be seen below:

↑ This is how the tutorial page looks at first. The instructions can be seen as broken down into various successive steps depending on the protocol content (ie. "materials" as seen in the image).

↑ As one scrolls down, some keywords start appear in green. These are the keywords linked up to an image. As one reads the instructions, s/he can click on these highlighted keywords to see a photograph of their execution.

Below are some images showing different instances of narration:

We also documented our mistakes and included these as possible errors

We hope that this platform will be useful to future iGEMers as well as to anyone who is new in the land of Synthetic Biology!