Difference between revisions of "Team:CSU Fort Collins/NoteBook/July"
| Line 65: | Line 65: | ||
<div class = 'Days'><h5>7/13/16:</h5></div> | <div class = 'Days'><h5>7/13/16:</h5></div> | ||
<div class = 'Info'><ul><li>Colony PCR performed on 10 EYFP-LVA+LrtA transformed colonies</li> | <div class = 'Info'><ul><li>Colony PCR performed on 10 EYFP-LVA+LrtA transformed colonies</li> | ||
| + | <li>PCR amplified Q1 and Q2 for infusion cloning</li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | <div class = 'Days'><h5>7/14/16:</h5></div> | ||
| + | <div class = 'Info'><ul><li>Ran Q1 and Q2 blocks in a 1.5% gel</li> | ||
| + | <li>Gel had no bands</li> | ||
| + | <li>PCR amplified for infusion cloning</li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| + | <div class = 'Days'><h5>7/15/16:</h5></div> | ||
| + | <div class = 'Info'><ul><li>Ran Q1 and Q2 blocks in a 1.5% gel</li> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/d/d1/CSU_Q1Q271516.png"> | ||
| + | <li>Infusion cloning on Quorum and PSB1C3</li> | ||
| + | <li>Transformed infusion mixture</li> | ||
| + | </ul> | ||
| + | </div> | ||
</html> | </html> | ||
Revision as of 14:53, 17 October 2016
CSU iGEM
July
7/6/16:
- PCRed out Lrta from Synechocystis PCC 6003
7/7/16:
- Ran Lrta PCR products on a 1% gel
7/8/16:
- Performed ligase cycling reaction on slr0168 and pSB1C3
- Ran all PCR products on 1%gel
- Digested Lrta(SpeI EcorI)
- Ligated Lrta and EYFP-LVA
- Transformed Lrta+EYFP-LVA ligation mixture
7/12/16:
- Dpn1 Digested slr0168 and pSB1C3
- Phosphorylated slr0168 and pSB1C3
- Performed ligase cycling reaction on phosphorylated mixture
- Transformed ligase cycling reaction products
- Digested EYFP-LVA(PstI)
- Ligated EYFP-LVA + LrtA
- Transformed EYFP-LVA+LrtA ligation mixture
- Performed colony PCR on 13 colonies with the Q1 block
- Ran PCR results on a 1.5% gel
7/13/16:
- Colony PCR performed on 10 EYFP-LVA+LrtA transformed colonies
- PCR amplified Q1 and Q2 for infusion cloning
7/14/16:
- Ran Q1 and Q2 blocks in a 1.5% gel
- Gel had no bands
- PCR amplified for infusion cloning
7/15/16:
- Ran Q1 and Q2 blocks in a 1.5% gel
- Infusion cloning on Quorum and PSB1C3
- Transformed infusion mixture
